血清CEA，CA153联合检测对肺癌诊断价值的探讨

目的：探讨血清CEA,CA153联合检测对肺癌的诊断价值。方法：用ACS：180SE全自动化学发光仪对60例原发性肺癌患者，53例肺良性疾病患者和70例正常对照分别测定CEA和CA153。结果：肺癌组CEA和CA153水平分别是15．75±18．81M，L和47．18＋39．52U／L均明显高于肺良性疾患组及正常对照组（P〈0.01）。肺癌组CEA，CA153联检阳性检出率为75．00％准确性为84．07％，均明显高于单项检测。结论：CEA和CA153联合检测可提高检测肺癌的敏感性和检测准确性，对肺癌的鉴别诊断、疗效监测具有较高的临床应用价值。     

重组人脑利钠肽治疗老年缺血性失代偿性心力衰竭

目的:探讨重组人脑利钠肽(rh BNP)治疗老年缺血性失代偿性心力衰竭的疗效及安全性。方法:选取心功能为NYHA IV级,经规范洋地黄强心、利尿剂利尿和硝酸甘油扩血管效果欠佳的老年缺血性失代偿性心力衰竭患者62例,随机分为2组:rh BNP治疗组(rh BNP组)31例,米力农治疗组(米力农组)31例。观察2组患者呼吸及心跳频率、NYHA心功能分级,左室射血分数(LVEF)、中心静脉压(CVP)、N末端B型钠尿肽(NT-pro BNP)水平并观察肝肾功能。结果:rh BNP组较米力农组患者的心率,肌酐及NT-pro BNP下降幅度明显增大,分别是(25.6±8.7)次/分,(18.5±7.3)次/分(p0.05);(68.2±11.8)μmol/L,(46.4±10.9)μmol/L(p0.01);(687.7±221.5)ng/L,(430.4±170.9)ng/L(p0.01)。rh BNP组较米力农组患者的LVEF的增加水平有明显差异,分别是(8.1±4.7)%,(5.5±3.3)%(p0.05)。而丙氨酸氨基转移酶、CVP、NYHA心功能分级及呼吸频率的改变则无明显差异,分别是(56.4±10.3)u/l,(47.8±2.6)u/l(p0.05);(5.4±3.3)cm H2o,(4.0±2.8)cm H2o(p0.05);(1.3±0.3),(1.0±0.4)(p0.05);(15.1±8.7)/分,(12.5±9.3)/分(p0.05)。结论:rh BNP治疗缺血性失代偿性心力衰竭疗效显著且安全性良好。     

血清CEA,CA153联合检测对肺癌诊断价值的探讨

目的:探讨血清CEA,CA153联合检测对肺癌的诊断价值.方法:用ACS:180SE全自动化学发光仪对60例原发性肺癌患者,53例肺良性疾病患者和70例正常对照分别测定CEA和CA153.结果:肺癌组CEA和CA153水平分别是15.75±18.81 μg/L和47.18±39.52 U/L均明显高于肺良性疾患组及正常对照组(P＜0.01).肺癌组CEA,CA153联检阳性检出率为75.00%准确性为84.07%,均明显高于单项检测.结论:CEA和CA153联合检测可提高检测肺癌的敏感性和检测准确性,对肺癌的鉴别诊断、疗效监测具有较高的临床应用价值.     

灿烂弧菌M-1菌株培养条件优化研究

采用梯度法比较了培养时间、温度、盐度、初始pH值、菌液起始浓度等对灿烂弧菌（Vibrio splendidus）M-1菌株生长的影响;采用正交试验法对Zobell 2216 E培养基组分进行了优化。结果表明：M-1菌株产最大活菌数的培养条件为：培养时间48 h、温度32℃、盐度40、初始pH值8.0、菌液起始浓度为1×105 CFU/mL;最佳培养基成分为：蛋白胨7.5 g/L、酵母浸出膏1.5 g/L、CuSO4 0.075 mg/L、CaCl2 0.04 g/L。     

血浆脑钠肽前体N端片段水平与心功能不全的相关性研究

目的研究患者血浆脑钠肽前体N端片段(NT-proBNP)在心血管病人心功能评价方面的作用。方法收录95个病人。根据病史、体格检查及超声心动图对病人进行评估。分为3组:A组(心功能正常,n=25)、B组(左室舒张功能不全,n=52)和C组(收缩功能不全,有或无舒张功能不全,n=18)。血浆NT-proBNP浓度在RochElecsys2010全自动测定仪上使用电化学发光法测定。结果在未分组的样本分析中,血浆NT-proBNP浓度与左室舒张末期内径(r=0.54,P=0.0016)、左房舒张末期内径(r=0.49,P=0.0016)、左室射血分数(r=0.59,P=0.0015)之间有显著相关性,与年龄没有明显的相关性。男性病人平均血浆NT-proBNP水平(945.5±3238.2)pg/mL和女性病人(722.0±1414.2)pg/mL之间差异没有显著性(P=0.66)。分组后分析显示:B组和C组病人的平均血浆NT-proBNP浓度分别是(128.6±194.8)pg/mL,(2496.2±4054.0)pg/mL与A组(39.6±46.1)pg/mL相比明显升高。结论在中国人群中,心血管病病人血浆NT-proBNP浓度的升高与心脏左室收缩、舒张功能受损及心腔扩大明显相关。     

吉林色洛河晚二叠世高镁安山岩SHRIMP锆石年代学及其地球化学特征

吉林色洛河地区发育高镁安山岩类,SHRIMP锆石U-Pb定年结果表明它们形成于晚二叠世（252±5Ma）.色洛河高镁安山岩类（SiO259.08%～65.67%）具有富MgO（3.68%～5.30%）,高Mg#值（0.62～0.68,平均0.66）,高Cr（203.17×10^-6～379.70×10^-6,平均258.79×10^-6）、Ni（98.13×10^-6～249.35×10^-6,平均137.00×10^-6）含量;富集大离子亲石元素（如Cs、K、Pb、Rb和Ba）,亏损高场强元素（如Ta、Ti、Nb、P）;富集LREE（Ce39.14×10^-6～48.74×10^-6）,强烈亏损HREE（Yb 0.95×10^-6～1.27×10^-6,Y 10.80×10^-6～13.13×10^-6,（La/Yb）N13.27～16.66）;但它们含有较低的Sr（158.62×10^-6～369.77×10^-6,平均258.52×10^-6）.它们属于中钾、钙碱性系列,具有明显的结晶分异和同化混染特征.上述地球化学特征表明它可能与消减沉积物流体交代形成富集地幔的部分熔融有关,其后又经历了分异和混染作用.这揭示它们形成于活动陆缘的构造背景,表明晚古生代末期古亚洲洋板块仍在消减,华北地块和佳木斯-兴凯地块（中亚造山带）最终的拼合时间可能在二叠纪之后.     

女性主义观照下的译者主体性——《简·爱》汉译案例分析

《简·爱》是女性作者夏洛蒂·勃朗特的具有女性主义特色的作品,本文尝试从女性主义翻译观的角度,试图对《简·爱》的两个中译本从语言表达和审美标准两个方面进行对比研究,所选的中译本译者分别为祝庆英（女）和吴钧燮（男）。译文的对比揭示出男女译者在操控语言和翻译策略选择上所体现出的译者主体性不同程度的发挥,旨在强调女性译者的女性主义特色,从而倡导女性主义翻译理论在中国的引入和实际运用。     

激素疗法治愈孤独症

从鼻腔吸入激素就能让一个人的生活态度更加积极、更乐于与他人交往？这正是纽约西奈山医学院研究人员的想法。该医学院的神经病学家埃里克·霍兰德和心理学家詹尼弗·巴特兹发现,让孤独症患者吸入后叶催产素（脑下垂体后叶分泌的一种激素）,能有效缓解他们令人困惑的症状：不能识别他人的情绪、无法与社会进行沟通、强迫性重复某些动作。     

皖东滁州、上腰铺埃达克质侵入岩年代学及地球化学特征：岩石成因与成矿意义

皖东的滁州、上腰铺岩体位于扬子地块东缘、毗邻郯庐断裂带东侧,与之伴生有一些铜（金或铁）矿化.它们的黑云母的40Ar-39Ar定年结果分别为127.17±0.40Ma,129.90±0.23 Ma.滁州、上腰铺侵入岩在地球化学特征上与埃达克岩基本一致：如SiO2＞56%,高Al2O3（14.84%～16.38%）、Sr（369×10^-6～1335×10^-6）、Sr/Y（43～185）与La/Yb（22～44）,但低Y（5.51×10^-6～11.0×10^-6）,Yb（0.51×10^-6～1.09×10^-6）,无明显Eu异常-正的Eu异常（δEu=-0.75～1.28）.另外,岩体中部分样品具有较高的MgO（2.23%～5.25%）、Mg#（53～68）和Cr（89.6×10^-6～206×10^-6）、Ni（43.0×10^-6～72.0×10^-6）,类似于高镁安山岩.这两个岩体的Nd-Sr同位素特征为：（^87 Sr/^86 Sr）I=0.7060～0.7067,εNd（t）=-11.53～-14.07,说明其由俯冲洋壳熔融形成的可能性较小.我们认为,滁州、上腰铺埃达克质侵入岩可能由拆沉下地壳熔融形成,熔融产生的岩浆在穿过上覆地幔的过程中,与地幔橄榄岩发生强烈相互作用,一方面,由于地幔橄榄岩的混染而使得埃达克质岩浆的MgO、Cr和Ni含量显著增高;另一方面,岩浆中的Fe2O3可能加入到地幔中,导致地幔的氧逸度（fo2）,）增高,地幔中金属硫化物被氧化,地幔中亲铜元素则以硫酸盐的形式进入熔体中.富含Cu、Au等成矿物质的熔体在快速上升到地壳浅处时,可能由于温度、压力或氧逸度（fo2）的降低,释放出Cu、Au等成矿物质,导致矿化.     

利用室温等离子体预处理和紫外光引发接枝聚合构造聚丙烯超疏水表面研究

采用紫外光引发自由基聚合液相接枝的方法,将甲基丙烯酸十二氟庚酯（G04）接枝聚合到使用室温等离子体预处理的聚丙烯（PP）片材表面,成功构建水接触角大于150°的超疏水表面。通过FTIR表征显示含氟聚合物以化学键的方式接枝在PP片材表面上。考察实验条件发现增大引发剂浓度、增加单体浓度以及提高反应温度均有利于提高表面接枝率,同时发现使用等离子体刻蚀的方法可以进一步提高表面接枝率。对接枝PP表面进行金相显微镜观察和水接触角（WCA）测量等表征,发现接枝PP表面形成了包括含氟聚合物的粗糙表面结构,水接触角随接枝率的增加而逐渐增大至150°以上。     

Correlation study of Carcino Embryonic Antigen &; Cancer Antigen 15.3 in pretreated female breast cancer patients

Carcino Embryonic Antigen (CEA) and Cancer Antigen 15.3 (CA15.3) are the most common tumor markers in breast cancer patients. Measurement of circulating tumor markers is a non-invasive quantitative method. Serum levels of CEA and CA 15.3 were studied in female breast cancer patients prior to treatment. To evaluate the utility of these markers, 207 Breast carcinoma patients belonging to all the stages were considered. Healthy age matched 75 female individuals formed the control group. The serum levels of CEA and CA 15.3 were analyzed by Enzyme Linked Immunosorbent Assay (ELISA). Results were taken and compared with stages, tumor size, node and grade. The serum CA 15.3 levels were significant in all the study parameters whereas serum CEA levels showed no significant changes with any of the parameters. Measurement of serum CA 15.3 levels showed significant correlation (24.8%) with advanced stages and larger tumor sizes, whereas serum CEA levels did not show any significant correlation in breast cancer patients prior to treatment.     

Prostate Specific Antigen in Cord Blood

Recent studies have demonstrated the presence of prostate specific antigen (PSA) in cord blood of male as well as female babies. The placental progesterone and estradiol up-regulate the synthesis and secretion of PSA in Placenta. This PSA is presumed to play a role in intrauterine growth of fetus by virtue of its proteolytic action on several substrates including insulin-like-growth-factor-binding-protein-3, insulin chains and Interleukin-2. This study was planned with the objective of correlating the levels of PSA in cord blood to gestation at delivery, the type of delivery and gender of the fetus. Fifty-seven cord blood samples were collected from the umbilical cord during delivery or mid-trimester abortion and analyzed for PSA using ‘Active PSA DSL-9700 ultra sensitive’ kit employing two-site immuno-radiometric assay principle and having a detection limit of 0.001 ng/ml. Mean PSA levels in cord blood were found to be 0.112 ± 0.027 ng/ml. The concentration of PSA in cord blood was found to be higher in case of higher gestational age, male baby and operative delivery. 50 % of cord bloods for female babies had PSA below detection limit (range <0.001–0.460 ng/ml), while all the male samples had detectable PSA (range 0.11–0.973 ng/ml). Higher Progesterone levels found in prenatal maternal blood in case of male babies may be responsible for the higher cord blood PSA. Mean cord blood PSA was 0.150 ± 0.150 ng/ml in forceps delivery and 0.078 ± 0.012 ng/ml in normal vaginal delivery. Forceps delivery causes much more stress and strain as compared to a normal vaginal delivery, resulting in increased levels of adrenal glucocorticoids, and therefore, higher cord blood PSA.     

重金属汞人工抗原的合成与鉴定

选用还原谷胱甘肽作为双功能螯合剂,与牛血清白蛋白偶联,成功螯合了Hg2+,制备汞完全抗原.通过等离子电感耦合法检测抗原与包被原中Hg2+的含量,采用考马斯亮蓝法及SDS-PAGE对抗原进行定性鉴定,并且用TNBS法测定氨基替换率.鉴定完成后用该抗原免疫动物,采用Hg-青霉素-O-VA、青霉素-OVA作包被原检测血清效价,效价大于1:160000.为制备重金属汞螫合物抗原特异性单克隆抗体提供新途径,为重金属免疫检测技术提供技术依托.     

Reconnoitring the Status of Prostate Specific Antigen and its Role in Women

Prostate specific antigen is considered to be a tumour marker having maximum utility and specificity for prostate cancer since decades. After the discovery of methods to quantify different molecular fractions of prostate specific antigen (PSA), its usefulness in diagnosing early prostate cancer cases has increased tremendously. The “specificity” of PSA, is now challenged by many studies which proved that PSA, once believed to be secreted exclusively by prostatic epithelium, is also present in females. The exact biological role of extraprostatic PSA is still debatable though many theories substantiated by in vitro evidence has been put forward. With the advent of ultrasensitive analytical techniques, PSA is now quantifiable in female serum in its various molecular forms and this has led to many assumptions of it being useful as a marker in female breast cancers. In a similar scenario to prostate cancer, the ratio of free to total PSA is shown to be useful in detecting early breast cancer cases. It is also shown to be a good prognostic indicator and a predictor of response to therapy and recurrence. Apart from its role in breast cancer, it has been advocated to be a marker of hyper androgenic states in women like hirsutism and polycystic ovarian syndrome. Conflicting reports regarding the role of extra prostatic PSA is accumulating but it has been proven beyond doubt that PSA is no longer specific and confined to prostate gland. Various studies have registered that PSA is an ubiquitous molecule, secreted by hormone responsive organs and its synthesis is stimulated by androgens and progesterone but not oestrogens. In this article, a review of various literatures is done about the presence of extra prostatic PSA, its probable role in those sites as well as its utility as a tumour marker in breast cancer.     

Carbohydrate Deficient Transferrin and Interleukin-6 as Predictors of Fibrosis in Alcohol Cirrhosis

The severity of alcoholic cirrhosis depends on the presence of liver inflammation and fibrogenesis. Previous studies have hypothesized that carbohydrate deficient transferrin can be used as marker of liver impairment in alcoholic liver disease patients. The present study was designed to assess whether carbohydrate deficient transferrin is associated with procollagen III peptide and predict fibrosis in alcohol cirrhosis patients. We enrolled 48 patients with alcoholic cirrhosis and 38 healthy controls. Serum carbohydrate deficient transferrin, procollagen III peptide and interleukin-6 levels were estimated in both groups. Serum carbohydrate deficient transferrin, procollagen III peptide and interleukin-6 were significantly increased in alcoholic cirrhosis patients compared to controls. Stepwise regression analysis showed that carbohydrate deficient transferrin (adjusted R² = 0.313, β = 0.362, p = 0.003) and interleukin-6 (adjusted R² = 0.194, β = 0.459, p = 0.001) were positively associated with procollagen III peptide when age, duration and amount of alcohol consumption were considered as covariates. We conclude that elevated carbohydrate deficient transferrin and interleukin-6 act as predictors of fibrosis in alcoholic cirrhosis.     

大肠粘膜增殖细胞核抗原的免疫组织化学研究

研究了增殖细胞核抗原(PCNA)免疫组织化学检测方法.发现PCNA可用于常规石腊包埋组织和冰冻组织,测量细胞的增殖活性.PCNA的检测受固定剂的影响.在石腊包埋组织4％付甲醛为最好,冰冻组织以甲醇为最佳.PCNA的阳性率与Ki67阳性率呈正相关.所以PCNA成为第一个能应用于常规病理组织,反映细胞增殖活性的指标.     

Systematic Differences Between Total and Free Prostate-Specific Antigen Immunoassays: Comparison Using Passing and Bablok Regression

Recent studies have shown that there are systematic differences among total and free prostate-specificantigen (PSA) immunoassays. In this study we analyzedintermethod differences in total PSA (tPSA) and free PSA(fPSA) measurement using ARCHITECT i2000SR (Abbott Diagnostics) and COBAS E601 (Roche Diagnostics). A number of 160 blood samples were tested for tPSA and 50 samples for fPSA (selecting only sampleswith tPSA: 4.1–10.0 μg/L). Passing–Bablok regression analysis was used to compare the two analytical methods fortPSA, fPSA and percentage of fPSA (%fPSA). A strong correlation was noticed between ARCHITECT i2000SR and COBAS E601 for tPSA, fPSA and %fPSA (r between 0.94 and 0.99). Concentrations of tPSA and fPSA measured by COBAS E601 were higher thanthose measured by ARCHITECT i2000SR with a bias of 0.8 μg/L for tPSA and 0.14 μg/L for fPSA. Analyzing therelative difference between methods for fPSA and %fPSA, COBAS E601 exceed a 10% relative difference limit. Our study confirms that there are differences in measured concentrations of tPSA and fPSA byvarious commercial methods. Because clinical judgment on subsequent diagnostic procedures, such as prostatebiopsy, is based on tPSA and fPSA results, tests harmonization should be a priority.     

论如何提高青少年足球运动员的意识发展

本文采用文献资料法、访谈法对影响青少年足球运动员意识水平发展的因素进行了分析,发现影响青少年足球运动员意识水平发展的因素主要分为客观因素和主观因素,其中客观因素包括：训练和比赛时周围的环境、教练员的训练水平和临场指挥、同队球员的配合、比赛时现场的气氛以及对手的情况等;主观因素包括：运动员的观察判断能力、技战术水平、智力水平、意志品质、性格特点等。并针对这些影响因素提出了改进措施,希望可以为我国青少年足球运动员足球意识水平的提高提供参考性的依据。