中药金芎原植物的研究

 A comparison of morphology, karyotype and the chemical compounds among “Jinxiong”, Ligasticum chuanxiong Hort. and L. chuanxiong cv. Fuxiong shows that “Jin- xiong”, like L. chuanxiong cv. Fuxiong, is a triploid and its karyotype is similar to that of L. chuanxiong with the formula K(2n)=33=24m+6sm+3st(SAT).   Thin-layer chromato- graphy of essential oils extracted from stem tubers confirms that the three taxa are extremely alike in chemical compounds. Using the features of leaves and the forms of stem tubers, they can be easily distinguished.  A new triploid cultivar of L. chuanxiong Hort. i.e.L. chuan-xiong cv. Jinxiong   is described for “Jinxiong” in the present paper.     

蜘蛛抱蛋属(百合科)的细胞分类学研究(1)——四川七个种的核型

In the work mitotic chromosomes in root-tips of 7 species native to Sichuan Pro- vince were examined and their karyotypes were analysed based on 7-8 cells at mitotic meta- phase, using Levan et al.'s (1964) nomenclature. The list of species and origin of the materials used in this work are provided in the appendix.  The micrographs of mitotic metaphase of all the materials are shown in Plates 1 and 2; the idiograms in Fig. 1, 1-9, and the parameters of chromosomes are provided in Tables 1-9.  All the chromosome countings and karyotypes in this paper are reported for the first time.      Characteristics of the karyotypes may be summarized as follows:      1.  2n=38 are found in all the materials except A. sichuanensis, which has 3 cytotypes, i.e, 2n=38+5B, 2n=38+lB and 2n=38+OB (Plate 2, 1-2, Fig. 1, 5-6).      2.  The karyotypes are of two major types: the karyotype of A. flaviflora falls into 3C in Stebbins's (1958, 197l) classification of karyotypes and those of all the other species into 2C. The two types are also different from each other in the number of large and medium-sized chromosomes pairs and morphology of the first pair of chromosomes (compare Plate 2, 5, Fig. 1, 9 with the other micrographs and idiograms).A. flaviflora with the karyotype 3C also differs from the other species in a series of gross morphological characters: the species is of a yellow and campanulate corolla.      3.  The species with caespitose leaves (A. caespitosa and A. omeiensis) have essentially the same karyotype, which is rather different from those of the species with scattered leaves. There are two pairs of small sm chromosomes (arm ratio ≥1.90) in the former karyotype (Tables 1 and 3), but all the small chromosomes are m or sm with arm ratio <1.80 in the latter karyotypes.     

中国特有的露蕊乌头亚属及铁破锣的染色体研究

The paper reports chromosomal number and chromosomal morphologies of annual Aconitum gymnandrum endemic to China and Beesia calthifolia for the first time.  Of the two spcies, chromosome number is same (X=8, 2n=16) and chromosome average lengths are 6.17μ , 10.73μ respectively.  The longest chromosome 1, the short chromosomes 3-5, 7 and the shortest chromosome 8 are metacentrical (m), the chro- mosomes 2, 6 are submetacentrical (sm), and the pairs 4, 5, 8 have satellites in the karyotype of A. gymnandrum.  In B. calthifolia, all of the chromosome 1-5 are the long m, the chromosomes 6, 8 are the short sm and the 7 is telocentrical (t). The pairs 3, 4, 6 have satellites.      According to the comparison of karyotypes of three subgenera—subgen. Para- conitum, subgen. Aconitum and subgen. Gymnaconitum in Aconitum, the evolution trend of chromosomes is further discussed.      Finally, the relationship between Aconitum and Beesia is also discussed in thispaper.     

白芷的核型研究及其分类意义

The present paper deals mainly with the karyotype analysis of five materials in Angelica dahurica collected in Yanbian of Jilin, Anguo of Hebei, Yuxian of Henan, Hangzhou of Zhejiang and Suining of Sichuan.  They are under the names “Dongbeidahuo”, “Qibaizhi”, “Yubaizhi”, ”Hangbaizhi” and “Chuanbaizhi” respe- ctively.  Among then “Dongbeidahuo” is a wild plant, which occurs in  northeastern China, and the others are cultivated as important crude drugs in some provinces. “Qi- Baizhi” and “Yubaizhi” have been identified as conspecific with the wild Baizhi-“Do- Ngbeidahuo” (A. dahurica) according to the external morphological features, whereas the other cultivated ones, “Hangbaizhi” and “Chuanbaizhi”, treated as a variety (A. dahu- rica var. formosana).      The results of karyotype analysis are shown in Plate 1, 2, with the formula 2n=22 =12 m+2 mSAT+4sm+4st. The karyotypes described here are constantly characterized by satellites attached to the fourth pair of metacentric chromosomes and differ from the pu- blished reports on the other species of the genus.  It is reasonable to say that the five materials collectively named “Baizhi” are taxonomically closely related to each other and could be regarded as conspecific.  Since the second chromosome pair is submetacen- tric in “Dongbeidahuo”, it may be justifiable to separate the wild plant from the cul- tivated ones and treat them as two separate varieties.     

11个四倍体赖草属物种的核型研究

对11个四倍体赖草属Leymus Hochst.物种的核型进行了研究，核型公式如下：沙生赖草L. arenarius (L.) Hochst., 2n=4x=28=18m+4sm+6st (4sat); 密穗赖草L. condensatus (J. Presl) A. Lve, 2n=4x=28=22m+4sm (2sat)+2st (2sat); 新生赖草L. innovatus (Beal) Pilg., 2n=4x=28=24m (4sat) +4sm (2sat);多枝赖草L. multicau     

中国海南岛13种菊科植物的细胞学研究

对海南岛13种菊科植物进行了染色体研究，对其中9种植物进行了核型分析，结果为: 树菊Tithoni-a diversifolia A.Gray，2n=34=26m+8sm（2sat）;鳢肠Eclipta prostrata（L.）L.，2n=22=18m+4sm;金腰箭Synedrella nodiflora（L.）Gaertn.，2n=40=6m+30sm（2sat）+4st;三叶鬼针草黄花类型Bidens pilosa L.（yel-low flower），2n=72=46m+26sm（2sat）;羽     

益母草的染色体数目

Leonurus japonicus Houtt. [L. heterophyllus Sweet, L. artemisia  (Lour.) S.  Y. Hu]  is one of the most important traditional Chinese medicines used as a remedy for gynaeco- logical disease since ancient times.  A cytological investigation on the species was carried out and the materials for chromosomal examination were collected from 26 localities in 20 provi- noes and autonomous regions of this country.  The number of chromosomes in root tip cell of the species was found to be 20 on the whole (Tab. 1:1), agreeing with those reported by Ma and al.[2] and probably by Chuang and al.[3] as well.      The genus Leonurus L. is variable in its  chromosomes with an aneuploidy of x=9, 10 and 12.  The present authors would propose that the primitive basic number of chromosome in the genus is 9, and thus both 10 and 12 are derived, for: (1) among the 9 species (including 1 sub- species) heretofore cytologically examined, x=9 occurring in 66.7%, x=10 occurring in 22.2%, while x=12 occurring only in 11.1%; (2) in generaclosely related to the genus under considera- tion, such as Panzeria, Galeobdolon and Lamium x=9 being the sole basic number.      But L. japonicus exhibits a mixoploidy of 2n=20 (occurring at the rate of 53.30% of the total amount of cells examined), 2n=18 (30.70%), and 2n=16 (15.99%) in our work. (Table 1).  Since the original basic number of  chromosome of the genus is 9 as proposed above, 2n= 20 would be considered as a derived one and the occurrence of 2n=18 probably suggests an early evolutionary trend of 2n=18→20 of the pecies in question.     

芍药属的研究(1)——国产几个野生种核型的报道

In the present paper 8 species with 15 populations of the genus Paeonia L. (if P. papaveracea and P. japonica are recognised as species) were collected from Sichuan, Shaanxi and Hebei provinces (see the Appendix for detail of the materials).  The micrographs of their somatic metaphase (also Mii in the case of P.veitchii) are shown in Plates 1-4, the karyo- type formulae, ranges of chromosome length and classification of karyotypes according to Steb- bins (1971) are shown in Table 5: the idiograms in Figs. 1-2, and the parameters of chromo- somes in Table 1-4.  The essential points are mentioned as follows:        (1)   Chromosomes of the various species in the section Modan have so far been examined and they are all diploid, the two species in the section Onaepia are also diploid, and thus tetraploids exist only in the section Paeonia.        (2)  Chromosomes in the genus Paeonia are relatively stable except for the differentiation of ploidy.  The karyotypes (Table 1-4) show no differences among different taxa in Sect. Modan and the same can also be said about the taxa in Sect. Paeonia (Table 1).  Not only are the karyotypes very similar, but also among the members within  either section have the same parameters of chromosomes, and, differences, if occur, are not statistically significant. Between the two sections, however, the situation is different. The arm ratios of the first pairs of chromosomes in Sect. Modan are 1.53, 1.52 and 1.48 (Table 1), but those in Sect. Paeonia are 1.12-1.28 (Table 2-4), 95% confidence limits are 1.46-1.60 for the section Modan and 1.07-1.28 (1.21-1.35 only for PB85078) for the section Paeonia, not overlapping, which indi- cates that the two sections have differentiated in respect of the first pairs of chromosomes.        (3)The population PB85024, which belongs to the P. obovata complex, has a karyotype of 2B (stebbins, 1971), which is a new one in the genus Paeonia. This karyotype is a stable one, for several individuals in the population are uniform in this respect, which shows that Steb- bins’ (1971) generalization that all the species in Paeonia have 2A does not hold true.        (4)  Three populations of P. obovata complex studied in this work from Sichuan and Shaanxi are all tetraploids, and one from Hebei is a diploid.  From the present work and the previcus reports, the materials from Japan and Korea, no matter whether flowers are pink or white, are diploids, those from Heilongjiang Province (with both pink and white flowers) (Liu Ming-yuan, personal communication) and from Heibei Province (with pink flowers) in China are also diploids, the one from Sakhalin (pink flowers) is tetraploid, those from Priamur of the Soviet Union are a tetraploid (with white flowers) and a diploid (with pink flowers), and those from Shaanxi (the Qinling Range) and western Sichuan (with both pink and white flowers) are all tetraploids.  As far as we have now known, ploidy in this parti- cular complex is correlated with the geographical distribution: diploids are found in the cen- tral part, tetraploids occur in the northern limits, and in the south  letraploids are the only cytotype.      (5)  The materials of P. mairei from western Sichuan and Shaanxi (the Qinling Range) are found all to be tetraploids, which shows that two cytotypes, diploid and tetraploid, exist in this  species,  but the geographical distribution pattern of these two cytotypes is to be revealed in the future investigation.     

仲彬草属和鹅观草属几个种的核型研究

 本文报道了仲彬草属2个种和鹅观草属3个种的核型，其核型均为首次报道。结果如下： Roegneria nutans，2n=4x=28=26m+2sm；R．abolinii，2n=4x=28=24m+4sm．R．aristiglumis， 2n=6x=42=32m+1Osm(2sat)；Kengyilia tahelacana，2n=6x=42=36m(2sat)+6sm(2sat)；K． zhoasuensis，2n=6x=42=34m(4sat)+8sm．根据核型特征，K．tahelacana和K．zhoasuensis两个六位体种归入K.engyilia属是正确的。     

蜘蛛抱蛋属的细胞分类学研究Ⅱ

文章报道了13种蜘蛛抱蛋属植物的染色体核型，并对属内核型进化规律作了总结。作者认为随体染色体和第1对染色体可以作为本属核型的特征染色体。染色体数目变异与花部式样密切相关。本属植物原始的染色体基数为x=19。此外，对非整倍性变异的主要机制也进行了讨论。     

葱属粗根组5种材料的核型研究

 本文分析了葱属Allium粗根组Sect．Bromatorrhiza Ekberg五群材料的核型。多星韭Allium   wallichii Kunth有两个类型：第一类型是二倍体，染色体组公式为AA，核型公式为K(2n)=2X=   14=2m(SAT)+2m+10sm，属2A型；第二类型是同源四倍体，染色体组公式为AAAA,  核型公   式为K(2n)＝4X＝28＝2m(SAT)+6m十20sm,属2A型。宽叶韭Allium hookeri Thwaites有   三个类型：  第一类型是双基数同源异源三倍体，染色体组公式为AAB1,核型公式为  K(2n)＝2X+   x'＝22＝(12sm+2t)十(1m十45m+1st+2t)，  属3A型；  第二类型也是双基数同源异源三倍   体，能配对的两个染色体组染色体大小和形态与第一类型大体相似，不能配对的一个染色体组染色体   大小和形态与第一类型有明显区别，其中至少有两条染色体发生了罗伯逊易位，出现一条很大的染色体    和一条很小的染色体，染色体组公式为AAB2，核型公式为K(2n)=2x+x'＝22=(12sm+2t)+   (3m+1sm十2st+2t)，属3A型；第三类型相当于第一类型染色体的自然加倍，是双基数同源异源   六倍体，染色体组公式为AAAAB1B1，核型公式为K(2n)=4X十2x'＝44＝(24sm+4t)十(2m+   8sm十2st+4t)，属3A型。     

当归属及近缘小属的核型演化及地理分布研究

 本文报道了当归属Angelica及3个近缘小属12种植物的核型，其中10种为首次报道。带岭当归A．dailingensis 2n＝22＝20m+2sm(SAT);丽江当属A．likiangensis 2n＝22＝18m+4sm; 青海当归A．nitida 2n＝22＝14m+4sm+4sm(SAT);林当归A．silvestris 2n＝22＝16m+4sm(SAT)+2st(SAT);紫花前胡A．decursiva 2n=22＝16m+4sm+2sm(SAT);秦岭当归A．tsinlingensis 2n＝22＝18m+4sm; 阿坝当归A．apaensis 2n＝22＝14m+6sm+2st(SAT);隆萼当归A．oncosepala2n＝4x＝44＝28m+12sm+4st，三小叶当归A．ternata 2n=22＝10m+8sm(SAT)+4st(SAT);柳叶芹Czernaevialaevigata 2n=22＝14m+6sm+2sm(SAT);短茎古当归Archangelica brevicaulis 2n＝22＝8m+2m(SAT)+4sm+4sm(SAT)+4st；高山芹Coelopleurum saxatile 2n＝28＝12m+6sm+10st。除带岭当归核型为1A型和高山芹为2B型外，其余种类均为2A型。根据染色体长度比和平均臂比绘制了本次和我们过去已报道的当归属及近缘属23种植物的核型散点图。据核型类型和近端着丝点的有无，把当归属20个种的核型分3组。并结合外部形态、花粉类型和地理分布，探讨了各近缘属的系统演化关系。     

黄枝油杉和矩鳞油杉的核型研究

本文研究了黄枝油杉Keteleeria calcarea Cheng et L．K．Fu．和矩鳞油杉Keteleeria   oblonga Cheng et L. K. Fu. 的核型。  并与江南油杉 K．cyclolepis Flous．和台湾油杉  K.   formosana Mast. 的核型比较，讨论四种油杉核型进化趋势。  黄枝油杉核型公式K(2n)=   24＝16m+8sm;矩鳞油杉核型公式K(2n)=24＝18m+6sm。  黄枝油杉在第1、3、6号   染色体短臂上具次缢痕;而矩鳞油杉在第2、4、6号染色体短臂上具次缢痕。二者均属较对称  的“2A“核型。  四种油杉核型进化趋势是江南油杉→矩鳞油杉→黄枝油杉→台湾油杉。     

四川当归属八种植物的核型及地理分布研究

本文首次报道四川八种当归属植物的染色体数目和核型。染色体基数x=11，除青海当归为四倍 体植物外，其余种类均为二倍体植物。金山当归  2n=22=20m+2sm；城口当归 2n=22=18m+2smsat+2sm；疏叶当归在不同地区有2种核型：2n=22=18m+4sm和 2n=22=16m+6sm；四川当归2n=22=16m+2smsat+4sm；茂汶当归2n=22=16m+6sm；青海当归 2n=4x=44=36m+8sm；当归2n= 22=14m+8sm；峨眉当归2n=22=10m+2sm+10st。除金山当归和 疏叶当归的部分居群核型为1A型外，其余种类均为2A型。根据核型的不对称性程度和外部形态分析 了各种类的演化水平，结合四川当归属植物的种类及地理分布，提出四川可能是当归属植物的原始中心和演化中心之一。     

贵州大树茶的核型变异与进化

本文对贵州大树茶7种1变种11类型的核型进行了分析。结果表明，这些种类均为二倍体2n＝30。五室茶Camellia quinquelocularis 2n＝30＝24m+6sm；四球茶C．tetracocca 2n＝30=22m+8sm；大理茶C．taliensis 2n=30＝22m+8sm；秃房茶C．gymnogyna 2n＝30＝22m+6sm+2st与2n=30＝20m+8sm+2st；假秃房茶C. gymnogynoides 2n＝30=22m+6sm+2st与2n＝30=20m+8sm+2st；榕江茶C. jungkiangensis 2n＝30+20m+8sm+2st；茶C．sinensis 2n=30＝20m+8sm+2st以及变种淡红花茶C．sinensis var．ruolla 2n＝30=20m+8sm+2st；均属2A核型。染色体结构变异在茶组植物演化中起了重要作用。所划分的两大类核型，即m和sm类与m，sm，和st类是与其子房室数，即5室和3室相一致的。根据核型的不对称性程度、外部形态及生化分析，探讨了各种类的亲缘关系与系统演化途径，论证了茶组植物的原产地是位于滇、桂、黔毗邻交汇处的云贵高原，探讨了茶组植物的分类学问题。     

几种萱草核型的比较研究

Some species of Hemerocallis were cultivated in the campus of Futan Un- iversity, including the evergreen H. aurantiaca Baker.  The original plant of H. auran- tiaca was introduced from Zhangzhou, southeastern Fukien Province.  The karyotype formula of H. aurantiaca is 2n=33=12m+9sm+3st+3T+6m(sat).   The  karyotype differs from those of summer-green or evergreen H. fulva (k(2n)=33=3M+21m+6sm +3T) and H. fulva var. kwanso (K(2n)=33=3M+l8m+6sm+3st+3T).  The vouchers are kept in FUS.