No effect of glutamine supplementation and hyperoxia on oxidative metabolism and performance during high-intensity exercise

Abstract

Glutamine enhances the exercise-induced expansion of the tricarboxylic acid intermediate pool. The aim of the present study was to determine whether oral glutamine, alone or in combination with hyperoxia, influenced oxidative metabolism and cycle time-trial performance. Eight participants consumed either placebo or 0.125 g · kg body mass^?1 of glutamine in 5 ml · kg body mass^?1 placebo 1 h before exercise in normoxic (control and glutamine respectively) or hyperoxic (FiO₂ = 50%; hyperoxia and hyperoxia + glutamine respectively) conditions. Participants then cycled for 6 min at 70% maximal oxygen uptake ([Vdot]O_2max) immediately before completing a brief high-intensity time-trial (～4 min) during which a pre-determined volume of work was completed as fast as possible. The increment in pulmonary oxygen uptake during the performance test (Δ[Vdot]O_2max, P = 0.02) and exercise performance (control: 243 s, s _x  = 7; glutamine: 242 s, s _x  = 3; hyperoxia: 231 s, s _x  = 3; hyperoxia + glutamine: 228 s, s _x  = 5; P < 0.01) were significantly improved in hyperoxic conditions. There was some evidence that glutamine ingestion increased Δ[Vdot]O_2max in normoxia, but not hyperoxia (interaction drink/FiO₂, P = 0.04), but there was no main effect or impact on performance. Overall, the data show no effect of glutamine ingestion either alone or in combination with hyperoxia, and thus no limiting effect of the tricarboxylic acid intermediate pool size, on oxidative metabolism and performance during maximal exercise.     

Effect of a carbohydrate mouthwash on running time-trial performance

The aim of the present study was to determine the effect of a carbohydrate mouthwash on running time-trial performance. On two separate occasions, seven recreationally active males (VO2max 57.8 ml x kg(-1) x min(-1), s = 3.7) completed a preloaded (15 min at 65%VO2max) time-trial of 45 min in duration on a motorized treadmill. At 6-min intervals during the preload and time-trial, participants were given either a 6% maltodextrin, 3% lemon juice solution (carbohydrate trial) or a 3% lemon juice placebo mouthwash (placebo trial) in a double-blind, randomized crossover design. Heart rate, oxygen consumption (VO2), respiratory exchange ratio (RER), and ratings of perceived exertion (RPE) were measured during the preload, and blood glucose and lactate were measured before and after the preload and time-trial. There were no significant differences in distance covered between trials (carbohydrate: 9333 m, s = 988; placebo: 9309 m, s = 993). Furthermore, there were no significant between-trial differences in heart rate and running speed during the time-trial, or VO2, RER or RPE during the preload. Blood lactate and glucose increased as a result of the exercise protocol, with no between-trial differences. In conclusion, there was no positive effect of a carbohydrate mouthwash on running performance of approximately 1 h duration.     

The effects of caffeine ingestion on performance time, speed and power during a laboratory-based 1 km cycling time-trial

There is little published data in relation to the effects of caffeine upon cycling performance, speed and power in trained cyclists, especially during cycling of approximately 60 s duration. To address this, eight trained cyclists performed a 1 km time-trial on an electronically braked cycle ergometer under three conditions: after ingestion of 5 mg x kg-1 caffeine, after ingestion of a placebo, or a control condition. The three time-trials were performed in a randomized order and performance time, mean speed, mean power and peak power were determined. Caffeine ingestion resulted in improved performance time (caffeine vs. placebo vs. control: 71.1 +/- 2.0 vs. 73.4 +/- 2.3 vs. 73.3 +/- 2.7 s; P = 0.02; mean +/- s). This change represented a 3.1% (95% confidence interval: 0.7-5.6) improvement compared with the placebo condition. Mean speed was also higher in the caffeine than placebo and control conditions (caffeine vs. placebo vs. control: 50.7 +/- 1.4 vs. 49.1 +/- 1.5 vs. 49.2 +/- 1.7 km x h-1; P = 0.0005). Mean power increased after caffeine ingestion (caffeine vs. placebo vs. control: 523 +/- 43 vs. 505 +/- 46 vs. 504 +/- 38 W; P = 0.007). Peak power also increased from 864 +/- 107 W (placebo) and 830 +/- 87 W (control) to 940 +/- 83 W after caffeine ingestion (P = 0.027). These results provide support for previous research that found improved performance after caffeine ingestion during short-duration high-intensity exercise. The magnitude of the improvements observed in our study could be due to our use of sport-specific ergometry, a tablet form and trained participants.     

Effects of inspiratory muscle training on time-trial performance in trained cyclists

We evaluated the effects of specific inspiratory muscle training on simulated time-trial performance in trained cyclists. Using a double-blind, placebo-controlled design, 16 male cyclists (VO2max = 64 +/- 2 ml x kg(-1) x min(-1); mean +/- s(x)) were assigned at random to either an experimental (pressure-threshold inspiratory muscle training) or sham-training control (placebo) group. Pulmonary function, maximum dynamic inspiratory muscle function and the physiological and perceptual responses to maximal incremental cycling were assessed. Simulated time-trial performance (20 and 40 km) was quantified as the time to complete pre-set amounts of work. Pulmonary function was unchanged after the intervention, but dynamic inspiratory muscle function improved in the inspiratory muscle training group (P < or = 0.05). After the intervention, the inspiratory muscle training group experienced a reduction in the perception of respiratory and peripheral effort (Borg CR10: 16 +/- 4% and 18 +/- 4% respectively; compared with placebo, P < or = 0.01) and completed the simulated 20 and 40 km time-trials faster than the placebo group [66 +/- 30 and 115 +/- 38 s (3.8 +/- 1.7% and 4.6 +/- 1.9%) faster respectively; P = 0.025 and 0.009]. These results support evidence that specific inspiratory muscle training attenuates the perceptual response to maximal incremental exercise. Furthermore, they provide evidence of performance enhancements in competitive cyclists after inspiratory muscle training.     

The effect of caffeine ingestion on human neutrophil oxidative burst responses following time-trial cycling

Following fixed-duration exercise of submaximal intensity, caffeine ingestion is associated with an attenuation of the exercise-induced decline in N-formyl-methionyl-phenyl-alanine (f-MLP) stimulated neutrophil oxidative burst. However, the response following high-intensity exhaustive exercise is unknown. Nine endurance-trained male cyclists ingested 6 mg caffeine or placebo per kilogram of body mass 60 min before cycling for 90 min at 70% of maximal oxygen consumption (VO2max) and then performing a time-trial requiring an energy expenditure equivalent to 30 min cycling at 70% maximum power output. Time-trial performance was 4% faster in the caffeine than in the placebo trial (P = 0.043). Caffeine was associated with an increased plasma adrenaline concentration after 90 min of exercise (P = 0.046) and immediately after the time-trial (P = 0.02). Caffeine was also associated with an increased serum caffeine concentration (P < 0.01) after 90 min of exercise and immediately after the time-trial, as well as 1 h after the time-trial. However, the f-MLP-stimulated neutrophil oxidative burst response fell after exercise in both trials (P = 0.002). There was no effect of caffeine on circulating leukocyte or neutrophil counts, but the lymphocyte count was significantly lower on caffeine (20%) after the time-trial (P = 0.003). Our results suggest that high-intensity exhaustive exercise negates the attenuation of the exercise-induced decrease in neutrophil oxidative burst responses previously observed when caffeine is ingested before exercise of fixed duration and intensity. This may be associated with the greater increase in adrenaline concentration observed in the present study.     

Carbohydrate and carbohydrate + protein for cycling time-trial performance

Carbohydrate intake during endurance exercise delays the onset of fatigue and improves performance. Two recent cycling studies have reported increased time to exhaustion when protein is ingested together with carbohydrate. The purpose of the present study was to test the hypothesis that ingestion of a carbohydrate + protein beverage will lead to significant improvements in cycling time-trial performance relative to placebo and carbohydrate alone. Thirteen cyclists completed 120 min of constant-load ergometer cycling. Thereafter, participants performed a time-trial in which they completed a set amount of work (7 kJ kg(-1)) as quickly as possible. Participants completed four experimental trials, the first for familiarization and then three randomized, double-blind treatments consisting of a placebo, carbohydrate, and carbohydrate + protein. Participants received 250 ml of beverage every 15 min during the constant-load ride. Time-trial performance for carbohydrate (37.1 min, s = 3.8) was significantly (P < 0.05) faster than placebo (39.7 min, s = 4.6). Time-trial performance for carbohydrate + protein (38.8 min, s = 5.5) was not significantly different from either placebo or carbohydrate. Ingestion of a carbohydrate beverage during two hours of constant-load cycling significantly enhanced subsequent time-trial performance compared with placebo. The carbohydrate + protein beverage provided no additional performance benefit.     

Effects of inspiratory muscle training on time-trial performance in trained cyclists

We evaluated the effects of specific inspiratory muscle training on simulated time-trial performance in trained cyclists. Using a double-blind, placebo-controlled design, 16 male cyclists (VO 2max = 64 - 2 ml·kg -1 ·min -1 ; mean - sx ¥ ) were assigned at random to either an experimental (pressure-threshold inspiratory muscle training) or sham-training control (placebo) group. Pulmonary function, maximum dynamic inspiratory muscle function and the physiological and perceptual responses to maximal incremental cycling were assessed. Simulated time-trial performance (20 and 40 km) was quantified as the time to complete pre-set amounts of work. Pulmonary function was unchanged after the intervention, but dynamic inspiratory muscle function improved in the inspiratory muscle training group ( P h 0.05). After the intervention, the inspiratory muscle training group experienced a reduction in the perception of respiratory and peripheral effort (Borg CR10: 16 - 4% and 18 - 4% respectively; compared with placebo, P h 0.01) and completed the simulated 20 and 40 km time-trials faster than the placebo group [66 - 30 and 115 - 38 s (3.8 - 1.7% and 4.6 - 1.9%) faster respectively; P = 0.025 and 0.009]. These results support evidence that specific inspiratory muscle training attenuates the perceptual response to maximal incremental exercise. Furthermore, they provide evidence of performance enhancements in competitive cyclists after inspiratory muscle training.     

Post-exercise ingestion of a unique, high molecular weight glucose polymer solution improves performance during a subsequent bout of cycling exercise

The aim of the present study was to determine the effect of post-exercise ingestion of a unique, high molecular weight glucose polymer solution, known to augment gastric emptying and post-exercise muscle glycogen re-synthesis, on performance during a subsequent bout of intense exercise. On three randomized visits, eight healthy men cycled to exhaustion at 73.0% (s = 1.3) maximal oxygen uptake (90 min, s = 15). Immediately after this, participants consumed a one-litre solution containing sugar-free flavoured water (control), 100 g of a low molecular weight glucose polymer or 100 g of a very high molecular weight glucose polymer, and rested on a bed for 2 h. After recovery, a 15-min time-trial was performed on a cycle ergometer, during which work output was determined. Post-exercise ingestion of the very high molecular weight glucose polymer solution resulted in faster and greater increases in blood glucose (P < 0.001) and serum insulin (P < 0.01) concentrations than the low molecular weight glucose polymer solution, and greater work output during the 15-min time-trial (164.1 kJ, s = 21.1) than both the sugar-free flavoured water (137.5 kJ, s = 24.2; P < 0.05) and the low molecular weight glucose polymer (149.4 kJ, s = 21.8; P < 0.05) solutions. These findings could be of practical importance for athletes wishing to optimize performance by facilitating rapid re-synthesis of the muscle glycogen store during recovery following prolonged sub-maximal exercise.     

A comparison of the cycling performance of cyclists and triathletes

The aim of this study was to compare the cycling performance of cyclists and triathletes. Each week for 3 weeks, and on different days, 25 highly trained male cyclists and 18 highly trained male triathletes performed: (1) an incremental exercise test on a cycle ergometer for the determination of peak oxygen consumption (VO2peak), peak power output and the first and second ventilatory thresholds, followed 15 min later by a sprint to volitional fatigue at 150% of peak power output; (2) a cycle to exhaustion test at the VO2peak power output; and (3) a 40-km cycle time-trial. There were no differences in VO2peak, peak power output, time to volitional fatigue at 150% of peak power output or time to exhaustion at VO2peak power output between the two groups. However, the cyclists had a significantly faster time to complete the 40-km time-trial (56:18 +/- 2:31 min:s; mean +/- s) than the triathletes (58:57 +/- 3:06 min:s; P < 0.01), which could be partially explained (r = 0.34-0.51; P < 0.05) by a significantly higher first (3.32 +/- 0.36 vs 3.08 +/- 0.36 l x min(-1)) and second ventilatory threshold (4.05 +/- 0.36 vs 3.81 +/- 0.29 l x min(-1); both P < 0.05) in the cyclists compared with the triathletes. In conclusion, cyclists may be able to perform better than triathletes in cycling time-trial events because they have higher first and second ventilatory thresholds.     

The effects of work:rest duration on physiological and perceptual responses during intermittent exercise and performance

In this study, we examined the effects of different work:rest durations during 20 min intermittent treadmill running and subsequent performance. Nine males (mean age 25.8 years, s = 6.8; body mass 73.9 kg, s = 8.8; stature 1.75 m, s = 0.05; VO(2max) 55.5 ml x kg(-1) x min(-1), s = 5.8) undertook repeated sprints at 120% of the speed at which VO(2max) was attained interspersed with passive recovery. The work:rest ratio was constant (1:1.5) with trials involving either short (6:9 s) or long (24:36 s) work:rest exercise protocols (total exercise time 8 min). Each trial was followed by a performance run to volitional exhaustion at the same running speed. Testing order was randomized and counterbalanced. Heart rate, oxygen consumption, respiratory exchange ratio, and blood glucose were similar between trials (P > 0.05). Blood lactate concentration was greater during the long than the short exercise protocol (P < 0.05), whereas blood pH was lower during the long than the short exercise protocol (7.28, s = 0.11 and 7.30, s = 0.03 at 20 min, respectively; P < 0.05). Perceptions of effort were greater throughout exercise for the long than the short exercise protocol (16.6, s = 1.4 and 15.1, s = 1.6 at 20 min, respectively; P < 0.05) and correlated with blood lactate (r = 0.43) and bicarbonate concentrations (r = 0.59; P < 0.05). Although blood lactate concentration at 20 min was related to performance time (r = - 0.56; P < 0.05), no differences were observed between trials for time to exhaustion (short exercise protocol: 95.8 s, s = 30.0; long exercise protocol: 92.0 s, s = 37.1) or physiological responses at exhaustion (P > 0.05). Our results demonstrate that 20 min of intermittent exercise involving a long work:rest duration elicits greater metabolic and perceptual strain than intermittent exercise undertaken with a short work:rest duration but does not affect subsequent run time to exhaustion.     

Effects of periodic carbohydrate ingestion on endurance and cognitive performances during a 40-km cycling time-trial under normobaric hypoxia in well-trained triathletes

The purpose of this study was to examine CHO ingestion on a cognitive task using a field-simulated time-trial (TT) under hypoxia in well-trained triathletes. Ten male triathletes (age: 22.1 ± 1.1 years; VO₂max: 59.4 ± 1.4 ml/kg/min) participated in this double-blind/crossover/counter-balanced design study. Participants completed 3 TT trials: 1) normoxic placebo (NPLA; FiO₂ = 20.9%), 2) hypoxic placebo (HPLA; FiO₂ = 16.3%), and 3) hypoxic CHO (HCHO; 6% CHO provided as 2 ml/kg/15 min; FiO₂ = 16.3%). During the TT, physiological responses (SpO₂, HR, RPE, and blood glucose/lactate), cognitive performance, and cerebral haemodynamics were measured. Hypoxia reduced TT performance by ~3.5–4% (p < 0.05), but CHO did not affect TT performance under hypoxia. For the cognitive task, CHO slightly preserved exercise-induced cognitive reaction speed but did not affect response accuracy during hypoxic exercise. However, CHO did not preserve the decreased Hb-Diff (cerebral blood flow, CBF) and increased HHb in the prefrontal lobe (p < 0.05) during hypoxic exercise, and CHO failed to preserve hypoxia-suppressed prefrontal CBF and tissue oxygen saturation. In conclusion, the present study demonstrates that CHO is effective in sustaining reaction speed for a cognitive task but not promoting TT performance during hypoxic exercise, which would be important for strategy-/decision-making when athletes compete at moderate high-altitude.     

The relationship between selected physiological variables of rowers and rowing performance as determined by a 2000 m ergometer test

The aim of this study was to establish the relationship between selected physiological variables of rowers and rowing performance as determined by a 2000 m time-trial on a Concept II Model B rowing ergometer. The participants were 13 male club standard oarsmen. Their mean (+/- s) age, body mass and height were 19.9+/-0.6 years, 73.1+/-6.6 kg and 180.5+/-4.6 cm respectively. The participants were tested on the rowing ergometer to determine their maximal oxygen uptake (VO2max), rowing economy, predicted velocity at VO2max, velocity and VO2 at the lactate threshold, and their velocity and VO2 at a blood lactate concentration of 4 mmol x l(-1). Percent body fat was estimated using the skinfold method. The velocity for the 2000 m performance test and the predicted velocities at the lactate threshold, at a blood lactate concentration of 4 mmol x l(-1) and at VO2max were 4.7+/-0.2, 3.9+/-0.2, 4.2+/-0.2 and 4.6+/-0.2 m x s(-1) respectively. A repeated-measures analysis of variance showed that the three predicted velocities were all significantly different from each other (P<0.05). The VO2max and lean body mass showed the highest correlation with the velocity for the 2000 m time-trial (r = 0.85). A stepwise multiple regression showed that VO2max was the best single predictor of the velocity for the 2000 m time-trial; a model incorporating VO2max explained 72% of the variability in 2000 m rowing performance. Our results suggest that rowers should devote time to the improvement of VO2max and lean body mass.     

Effect of glucose polymer ingestion on energy and fluid balance during exercise

Nine male triathletes were studied during 160 min of exercise at 65% VO2 max on two occasions to examine the effect of glucose polymer ingestion on energy and fluid balance. During one trial they received 200 ml of a 10% glucose polymer solution at 20 min intervals during exercise (CHO), while in the other they received an equal volume of a sweet placebo (CON). On average, blood glucose levels (CON = 4.2 +/- 0.2 mmol l-1, CHO = 4.8 +/- 0.1, mean +/- S.E.) and respiratory exchange ratios (CON = 0.84 +/- 0.01, CHO = 0.87 +/- 0.01) during exercise were higher (P less than 0.05) as a result of the glucose polymer ingestion. There were no differences between trials, however, in the estimated plasma volume changes during exercise. Exercise time to exhaustion at an intensity corresponding to 110% VO2 max, performed 5 min after the submaximal exercise, was not influenced by glucose polymer ingestion. Relative to a control exercise bout conducted without prior exercise, however, sprint performance and postexercise blood lactate accumulation were impaired in both trials. It is concluded that glucose polymer ingestion maintains blood glucose levels and a high rate of carbohydrate oxidation during prolonged exercise, without compromising fluid balance.     

Sodium bicarbonate ingestion does not alter the slow component of oxygen uptake kinetics in professional cyclists

We examined the effects of pre-exercise sodium bicarbonate (NaHCO3) ingestion on the slow component of oxygen uptake (VO2) kinetics in seven professional road cyclists during intense exercise. One hour after ingesting either a placebo or NaHCO3 (0.3 g x kg body mass(-1)), each cyclist (age, 25 +/- 2 years; VO2max, 74.7 +/- 5.9 ml x kg(-1) x min(-1); mean +/- s) performed two bouts of 6 min duration at an intensity of 90% VO2max interspersed by 8 min of active recovery. Gas exchange and blood data (pH, blood lactate concentration and [HCO3-]) were collected during the tests. In both bouts, the slow component of VO2 was defined as the difference between end-exercise VO2 and the VO2 at the end of the third minute. No significant difference was found in the slow component of VO2 between conditions in the first (NaHCO3, 210 +/- 69 ml; placebo, 239 +/- 105 ml) or second trial (NaHCO3, 123 +/- 88 ml; placebo, 197 +/- 101 ml). In conclusion, pre-exercise NaHCO3 ingestion did not significantly attenuate the VO2 slow component of professional road cyclists during high-intensity exercise.     

Effect of age on 16.1-km time-trial performance

In this study, we assessed the performance of trained senior (n = 6) and veteran (n = 6) cyclists (mean age 28 years, s = 3 and 57 years, s = 4 respectively). Each competitor completed two cycling tests, a ramped peak aerobic test and an indoor 16.1-km time-trial. The tests were performed using a Kingcycle ergometer with the cyclists riding their own bicycle fitted with an SRM powermeter. Power output, heart rate, and gas exchange variables were recorded continuously and blood lactate concentration [HLa] was assessed 3 min after the peak ramped test and at 2.5-min intervals during the time-trial. Peak values for power output (RMP(max)), heart rate (HR(peak)), oxygen uptake (VO2(peak)), and ventilation (V(Epeak)) attained during the ramped test were higher in the senior group (P < 0.05), whereas [HLa](peak), RER(peak), V(E): VO2(peak), and economy(peak) were similar between groups (P > 0.05). Time-trial values (mean for duration of race) for power output (W(TT)), heart rate (HR(TT)), VO2 (VO(2TT)), and V(E) (V(ETT)) were higher in the seniors (P < 0.05), but [HLa](TT), RER(TT), V(ETT): VO2(TT), and economy(TT) were similar between the groups (P > 0.05). Time-trial exercise intensity, expressed as %RMP(max), %HR(peak), % VO2(peak), and % V(Epeak), was similar (P > 0.05) for seniors and veterans (W(TT): 81%, s = 2 vs. 78%, s = 8; HR(TT): 96%, s = 4 vs. 94%, s = 4; VO2(TT): 92%, s = 4 vs. 95%, s = 10; V(ETT): 89%, s = 8 vs. 85%, s = 8, respectively). Overall, seniors attained higher absolute values for power output, heart rate, VO2, and V(E) but not blood lactate concentration, respiratory exchange ratio (RER), V(E): VO2, and economy. Veterans did not accommodate age-related declines in time trial performance by maintaining higher relative exercise intensity.     

The effect of pre-exercise carbohydrate feedings on the intensity that elicits maximal fat oxidation

The aim of the present study was to examine the effect of ingesting 75 g of glucose 45 min before the start of a graded exercise test to exhaustion on the determination of the intensity that elicits maximal fat oxidation (Fatmax). Eleven moderately trained individuals (VO2max: 58.9 +/- 1.0 ml x kg(-1) x min(-1); mean +/- sx), who had fasted overnight, performed two graded exercise tests to exhaustion, one 45 min after ingesting a placebo drink and one 45 min after ingesting 75 g of carbohydrate in the form of glucose. The tests started at 95 W and the workload was increased by 35 W every 3 min. Gas exchange measures and heart rate were recorded throughout exercise. Fat oxidation rates were calculated using stoichiometric equations. Blood samples were collected at rest and at the end of each stage of the test. Maximal fat oxidation rates decreased from 0.46 +/- 0.06 to 0.33 +/- 0.06 g min(-1) when carbohydrate was ingested before the start of exercise (P < 0.01). There was also a decrease in the intensity which elicited maximal fat oxidation (60.1 +/- 1.9% vs 52.0+3.4% VO2max) after carbohydrate ingestion (P < 0.05). Maximal power output was higher in the carbohydrate than in the placebo trial (346 +/- 12 vs 332 +/- 12 W) (P < 0.05). In conclusion, the ingestion of 75 g of carbohydrate 45 min before the onset of exercise decreased Fatmax by 14%, while the maximal rate of fat oxidation decreased by 28%.     

Effects of hyperoxia during recovery from 5×30-s bouts of maximal-intensity exercise

We test the hypothesis that breathing oxygen-enriched air (F(I)O(2) = 100%) maintains exercise performance and reduces fatigue during intervals of maximal-intensity cycling. Ten well-trained male cyclists (age 25 ± 3 years; peak oxygen uptake 64.8 ± 6.2 ml · kg(-1) · min(-1); mean ± s) were exposed to either hyperoxic or normoxic air during the 6-min intervals between five 30-s sessions of cycling at maximal intensity. The concentrations of lactate and hydrogen ions [H(+)], pH, base excess, oxygen partial pressure, and oxygen saturation in the blood were assessed before and after these sprints. The peak (P = 0.62) and mean power outputs (P = 0.83) with hyperoxic and normoxic air did not differ. The partial pressure of oxygen was 4.2-fold higher after inhaling hyperoxic air, whereas lactate concentration, pH, [H(+)], and base excess (P ≥ 0.17) were not influenced. Perceived exertion towards the end of the 6-min periods after the fourth and fifth sprints (P < 0.05) was lower with hyperoxia than normoxia (P < 0.05). These findings demonstrate that the peak and mean power outputs of athletes performing intervals of maximal-intensity cycling are not improved by inhalation of oxygen-enriched air during recovery.     

The effect of caffeine ingestion on 8 km run performance in a field setting

The aim of this study was to assess the effect of caffeine ingestion on 8 km run performance using an ecologically valid test protocol. A randomized double-blind crossover study was conducted involving eight male distance runners. The participants ran an 8 km race 1 h after ingesting a placebo capsule, a caffeine capsule (3 mg x kg(-1) body mass) or no supplement. Heart rate was recorded at 5 s intervals throughout the race. Blood lactate concentration and ratings of perceived exertion were recorded after exercise. A repeated-measures analysis of variance (ANOVA) identified a significant treatment effect for 8 km performance time (P < 0.05); caffeine resulted in a mean improvement of 23.8 s (95% confidence interval [CI] = 13.1 to 34.5 s) in 8 km performance time (1.2% improvement, 95% CI = 0.7 to 1.8%). In addition, a two-way (time x condition) repeated-measures ANOVA identified a significantly higher blood lactate concentration 3 min after exercise during the caffeine trial (P < 0.05). We conclude that ingestion of 3 mg . kg(-1) body mass of caffeine can improve absolute 8 km run performance in an ecologically valid race setting.     

The effects of different doses of caffeine on endurance cycling time trial performance

This study investigated the effects of two different doses of caffeine on endurance cycle time trial performance in male athletes. Using a randomised, placebo-controlled, double-blind crossover study design, sixteen well-trained and familiarised male cyclists (Mean ± s: Age = 32.6 ± 8.3 years; Body mass = 78.5 ± 6.0 kg; Height = 180.9 ± 5.5 cm VO2(peak) = 60.4 ± 4.1 ml x kg(-1) x min(-1)) completed three experimental trials, following training and dietary standardisation. Participants ingested either a placebo, or 3 or 6 mg x kg(-1) body mass of caffeine 90 min prior to completing a set amount of work equivalent to 75% of peak sustainable power output for 60 min. Exercise performance was significantly (P < 0.05) improved with both caffeine treatments as compared to placebo (4.2% with 3 mg x kg(-1) body mass and 2.9% with 6 mg x kg(-1) body mass). The difference between the two caffeine doses was not statistically significant (P = 0.24). Caffeine ingestion at either dose resulted in significantly higher heart rate values than the placebo conditions (P < 0.05), but no statistically significant treatment effects in ratings of perceived exertion (RPE) were observed (P = 0.39). A caffeine dose of 3 mg x kg(-1) body mass appears to improve cycling performance in well-trained and familiarised athletes. Doubling the dose to 6 mg x kg(-1) body mass does not confer any additional improvements in performance.     

The effects of work-rest duration on intermittent exercise and subsequent performance

This study examined the effects of different work - rest durations during 40 min intermittent treadmill exercise and subsequent running performance. Eight males (mean +/- s: age 24.3 +/- 2.0 years, body mass 79.4 +/- 7.0 kg, height 1.77 +/- 0.05 m) undertook intermittent exercise involving repeated sprints at 120% of the speed at which maximal oxygen uptake (nu-VO2max) was attained with passive recovery between each one. The work - rest ratio was constant at 1:1.5 with trials involving short (6:9 s), medium (12:18 s) or long (24:36 s) work - rest durations. Each trial was followed by a performance run to volitional exhaustion at 150% nu-VO2max. After 40 min, mean exercise intensity was greater during the long (68.4 +/- 9.3%) than the short work - rest trial (54.9 +/- 8.1% VO2max; P < 0.05). Blood lactate concentration at 10 min was higher in the long and medium than in the short work - rest trial (6.1 +/- 0.8, 5.2 +/- 0.9, 4.5 +/- 1.3 mmol x l(-1), respectively; P < 0.05). The respiratory exchange ratio was consistently higher during the long than during the medium and short work - rest trials (P < 0.05). Plasma glucose concentration was higher in the long and medium than in the short work - rest trial after 40 min of exercise (5.6 +/- 0.1, 6.6 +/- 0.2 and 5.3 +/- 0.5 mmol x l(-1), respectively; P < 0.05). No differences were observed between trials for performance time (72.7 +/- 14.9, 63.2 +/- 13.2, 57.6 +/- 13.5 s for the short, medium and long work - rest trial, respectively; P = 0.17), although a relationship between performance time and 40 min plasma glucose was observed (P < 0.05). The results show that 40 min of intermittent exercise involving long and medium work - rest durations elicits greater physiological strain and carbohydrate utilization than the same amount of intermittent exercise undertaken with a short work-rest duration.