稀释分离法鉴定嗜鳍黄杆菌

水稻稻瘟病是我国水稻的重要病害．目前,水稻抗瘟性品种的利用和化学农药的使用仍然是防治稻瘟病行之有效的措施,但因抗瘟品种的单一化、稻瘟病菌生理小种遗传的复杂性和致病性的多样性,以及化学农药的毒性和病原茼的抗药性,使得水稻抗瘟性品种和化学农药的使用在生产上都受到一定的限制．为此,水稻稻瘟病的生物防治特别是生物农药的研究与开发是水稻生产可持续发展的需要．本试验用稀释分离法进行拮抗菌株的分离,选择了拮抗效果最好的菌株做形态学及生理生化特征鉴定．通过对菌株SY42的培养性状及生理生化特征的测定,初步鉴定其为嗜鳍黄杆菌．     

浅谈水稻稻瘟病发生的外在影响因素

认为气象条件和耕作栽培管理技术是水稻稻瘟病引发和流行的外在影响因素。     

浅谈水稻稻瘟病发生的外在影响因素

认为气象条件和耕作栽培管理技术是水稻稻瘟病引发和流行的外在影响因素     

长白山野生藜芦提取液对水稻稻瘟病抑菌效应的研究

为明确押菌植物对水稻稻瘟病菌的抑制作用,文章以长白山的藜芦为材料,对藜芦根、茎、叶采用70％乙醇、60％丙酮、沸水进行了提取,并用提取液对水稻稻瘟病菌的抑菌效应进行了测定.结果表明,藜芦的不同药用部分抑菌作用存在明显差异,叶抑菌作用最强,茎次之,根部的抑菌作用最小;提取液的抑菌效果也存在差异,70％乙醇提取液的抑菌作用最强.     

中熟中粳糯新品种盐糯12的选育与高产栽培技术

盐糯12系江苏省盐城市盐都区农科所育成的中熟中粳糯稻新品种，高产、优质、多抗，适应性广。株高95cm，千粒重26g，分蘖性强，穗大粒多，对稻瘟病田间抗性较强，尤其中高抗水稻条纹叶枯病。2006年1月通过江苏省农作物品种审定委员会审定，并已审请品种权保护，公告号为CNA001692E。     

春雷霉素WP防治水稻稻瘟病田间药效试验

进行了春雷霉素防治水稻稻瘟病试验,试验结果表明,2％春雷霉素WP对水稻稻穗瘟有较好的防效,防效达91．0％-94．2％,试验剂量下对水稻安全无害,可以大面积推广应用,生产上推荐用量为112．45—181．25g／hm^2。     

水稻稻瘟病与气候条件分析

针对稻瘟病对水稻的严重危害,通过查找宜春市植保部门稻瘟病重发年与气象部门1971～2000年(共计30年)的1～2月平均气温资料对比分析,认为暖冬及水稻生长关键期的雨日雨量是诱发稻瘟病呈现两个发病高峰的重要因素,并由此提出具有针对性的防治对策及建议.     

PglnA启动子控制的ω-3去饱和酶基因的转基因集胞藻的构建

为了能在常温下提高多不饱和脂肪酸产量,以及用单交换的方式在集胞藻PCC6803的染色体上整合入基因片段,本研究首先构建出链霉素抗性基因、常温启动子、desB基因片段依次相连的质粒,然后以单交换的方式将这些片段整合在集胞藻Pcc6803的染色体上,最后成功筛选出了转基因藻.该研究为常温下大量表达多不饱和脂肪酸奠定了基础.     

CHEF Ⅲ型脉冲电泳仪获得清晰电泳效果的实验探索

CHEF Ⅲ型脉冲电泳仪是微生物种类鉴定、DNA大分子分离等基础科学研究中一个不可或缺的重要工具。该仪器可以快速、高效、准确地分离200 kb到6 Mb的DNA分子。因此,在染色体数目及染色体大小的确定、核型分析、遗传图谱分析、同源性检测分析等方面有关键性的作用。然而在实验过程中要使DNA分子完全、有效地分离,得到清晰、高效的条带却并非易事。本实验基于这些问题,经过一系列的探索研究,找到了稻瘟病菌及分子量标准酵母菌染色体分离所需要的最适条件,该条件能为分离其他真菌染色体提供借鉴与参考。     

CHEⅢ型脉冲电泳仪获得清晰电泳效果的实验探索

CHEF Ⅲ型脉冲电泳仪是微生物种类鉴定、DNA大分子分离等基础科学研究中一个不可或缺的重要工具。该仪器可以快速、高效、准确地分离200 kb到6 Mb的DNA分子。因此,在染色体数目及染色体大小的确定、核型分析、遗传图谱分析、同源性检测分析等方面有关键性的作用。然而在实验过程中要使DNA分子完全、有效地分离,得到清晰、高效的条带却并非易事。本实验基于这些问题,经过一系列的探索研究,找到了稻瘟病菌及分子量标准酵母菌染色体分离所需要的最适条件,该条件能为分离其他真菌染色体提供借鉴与参考。     

Numerical Simulation of Concrete Plate Damaged Under Explosive Loading

The grisliness after-effects can be induced by explosion accident with the collapsing of the structures, the demolishing of the equipments and the casualty of the human beings. Isolation belt constructed between the blast point and the construction is one of the useful design schemes for blast resistance. The nonlinear procedure ANSYS/LSDYNA970 is used to simulate the contact detonation and the isolation belt of blast resistance filled with the air or water respectively. The results indicate that the maximal damage can be caused by the contact detonation, and the isolation belt of blast resistent filled with water can reduce the damage greatly.     

Numerical Simulation of Concrete Plate Damaged Under Explosive Loading

The grisliness after-effects can be induced by explosion accident with the collapsing of the structures, the demolishing of the equipments and the casualty of the human beings. Isolation belt constructed between the blast point and the construction is one of the useful design schemes for blast resistance. The nonlinear procedure ANSYS/LSDYNA970 is used to simulate the contact detonation and the isolation belt of blast resistance filled with the air or water respectively. The results indicate that the maximal damage can be caused by the contact detonation, and the isolation belt of blast resistent filled with water can reduce the damage greatly.     

北营新1号高炉高风温生产实践

北营新1号高炉通过优化高炉煤气处理流程,配置旋切顶燃式热风炉,采用前置预热炉、热管换热器分别预热助燃空气和煤气,使用带横梁的炉箅子技术、管道保温技术以及优化烧炉技术等,为送风温度达到1250℃创造了条件。高炉在精料基础上,通过控制合理炉腹煤气量指数和理论燃烧温度,优化煤气流分布,规范高炉操作等措施,2013年全年平均风温1248℃,达到国内同型高炉领先水平。     

武钢4#高炉专家系统参数的检测及对策

本文介绍了高炉专家系统的构成及其在武钢4^#高炉上的应用情况，叙述了专家系统所需的主要工艺参数及其对指导高炉生产的重要性，另外，本文重点叙述了专家系统设计的工艺参数采集的过程，详叙了在高炉实际检测中，对取样难度较大的工艺参数的检测手段及方法，并陈述了在专家系统的实际应用中遇到的问题及看法。     

Rate-sensitive numerical analysis of dynamic responses of arched blast doors subjected to blast loading

Current practice in analysis and design of blast doors subjected to blast loading considers only simple boundary conditions and material properties. The boundary conditions and material properties, in fact, have considerable influence on the response of blast doors subjected to blast loading. In this paper, the dynamic responses of a reinforced concrete arched blast door under blast loading were analyzed by the finite element program ABAQUS, combined with a previously developed elasto-viscoplastic rate-sensitive material model. And the effect of the surrounding rock mass and contact effect of the doorframe were also taken into account in the simulation. It is demonstrated that the strain-rate effect has considerable influence on the response of reinforced concrete blast door subjected to blast loading and must be taken into account in the analysis.     

浓缩塔控爆拆除

武钢二炼钢厂浊循环水二次浓缩塔的爆破环境十分恶劣，通过优化爆破设计、精心施工，达到了完全准爆的预想效果。     

高炉TRT控制系统模型仿真研究

根据高炉TRT工艺和影响高炉顶压稳定的因素,以及对高炉TRT系统的管路分析,建立高炉顶压TRT系统数学模型。应用Matlab对静叶单独调节高炉顶压时系统的数学模型进行仿真验证,为进一步改善高炉顶压的稳定提供理论依据。     

Analysis of blast wave propagation inside tunnel

The explosion inside tunnel would generate blast wave which transmits through the longitudinal tunnel. Because of the close-in effects of the tunnel and the reflection by the confining tunnel structure, blast wave propagation inside tunnel is distinguished from that in air. When the explosion happens inside tunnel, the overpressure peak is higher than that of explosion happening in air. The continuance time of the blast wave also becomes longer. With the help of the numerical simulation finite element software LS-DYNA, a three-dimensional nonlinear dynamic simulation analysis for an explosion experiment inside tunnel was carried out. LS-DYNA is a fully integrated analysis program specifically designed for nonlinear dynamics and large strain problems. Compared with the experimental results, the simulation results have made the material parameters of numerical simulation model available. By using the model and the same material parameters, many results were adopted by calculating the model under different TNT explosion dynamites. Then the method of dimensional analysis was used for the simulation results. As overpressures of the explosion blast wave are the governing factor in the tunnel responses, a formula for the explosion blast wave over-pressure at a certain distance from the detonation center point inside the tunnel was derived by using the dimensional analysis theory. By comparing the results computed by the formula with experimental results which were obtained before, the formula was proved to be very applicable at some instance. The research may be helpful to estimate rapidly the effect of internal explosion of tunnel on the structure.