Effect of a carbohydrate mouthwash on running time-trial performance

Abstract

The aim of the present study was to determine the effect of a carbohydrate mouthwash on running time-trial performance. On two separate occasions, seven recreationally active males ([Vdot]O_2max 57.8 ml · kg^?1 · min^?1, s = 3.7) completed a preloaded (15 min at 65%[Vdot]O_2max) time-trial of 45 min in duration on a motorized treadmill. At 6-min intervals during the preload and time-trial, participants were given either a 6% maltodextrin, 3% lemon juice solution (carbohydrate trial) or a 3% lemon juice placebo mouthwash (placebo trial) in a double-blind, randomized crossover design. Heart rate, oxygen consumption ([Vdot]O₂), respiratory exchange ratio (RER), and ratings of perceived exertion (RPE) were measured during the preload, and blood glucose and lactate were measured before and after the preload and time-trial. There were no significant differences in distance covered between trials (carbohydrate: 9333 m, s = 988; placebo: 9309 m, s = 993). Furthermore, there were no significant between-trial differences in heart rate and running speed during the time-trial, or [Vdot]O₂, RER or RPE during the preload. Blood lactate and glucose increased as a result of the exercise protocol, with no between-trial differences. In conclusion, there was no positive effect of a carbohydrate mouthwash on running performance of ～1 h duration.     

The effects of prior incremental cycle exercise on the physiological responses during incremental running to exhaustion: relevance for sprint triathlon performance

It is common for the physiological working capacity of a triathlete when cycling and running to be assessed on two separate days. The aim of this study was to establish whether an incremental running test to exhaustion has a negative effect after a 5 h recovery from an incremental cycling test. Eight moderately trained triathletes (age, 26.2 +/- 3.4 years; body mass, 67.3 +/- 9.1 kg; VO2max when cycling, 59 +/- 13 ml x kg x min(-1); mean +/- s) completed an incremental running test 5 h after an incremental cycling test (fatigue) as well as an incremental running test without previous activity (control). Maximum running speed, maximal oxygen uptake (VO2max) and the lactate threshold were determined for each incremental running test and correlated with the average speed during a 5 km run, which was performed immediately after a 20 km cycling time-trial, as in a sprint triathlon. There were no significant differences in maximum running speed, VO2max or the lactate threshold in either incremental running test (control or fatigue). Furthermore, good agreement was found for each physiological variable in both the control and fatigue tests. For the fatigue test, there were significant correlations between the average speed during a 5 km run and both VO2max expressed in absolute terms (r = 0.83) and the lactate threshold (r = 0.88). However, maximum running speed correlated most strongly with the average speed during a 5 km run (r = 0.96). The results of this study indicate that, under controlled conditions, an incremental running test can be performed successfully 5 h after an incremental cycling test to exhaustion. Also, the maximum running speed achieved during an incremental running test is the variable that correlates most strongly with the average running speed during a 5 km run after a 20 km cycling time-trial in well-trained triathletes.     

The effects of frequency of encouragement on performance during maximal exercise testing

The aim of this study was to determine the effects of frequency of verbal encouragement during maximal exercise testing. Twenty-eight participants (12 males, 16 females) aged 20.9 +/- 1.5 years (mean +/- s) performed a maximal exercise test (VO2max) on a treadmill without any verbal encouragement. The participants were matched according to their pre-test VO2max and placed into either a control group or one of three experimental groups. They performed a second exercise test (post-test) 1 week later. During the second test, the control group received no verbal encouragement; the 20 s (20E), 60 s (60E) and 180 s (180E) encouragement groups received verbal encouragement every 20, 60 and 180 s, respectively, beginning with stage 3 of the exercise test. Relative VO2max, exercise time, blood lactate concentration, respiratory exchange ratio (RER) and ratings of perceived exertion (RPE) were not significantly different from the first test to the second test for the control group without verbal encouragement and the 180E group that received infrequent encouragement. Post-test values were significantly higher than pre-test values for the 20E and 60E groups. The post-test values of the 20E group were significantly higher than their pre-test values for relative VO2max (P < 0.001), exercise time (P < 0.0001), blood lactate concentration (P < 0.05), RER (P < 0.01) and RPE (P < 0.0001); this was also the case for the 60E group for relative VO2max (P < 0.01), blood lactate concentration (P < 0.05), RER (P < 0.05) and RPE (P < 0.05). The results suggest that frequent verbal encouragement (every 20 s and 60 s in the present study) leads to significantly greater maximum effort in a treadmill test than when no encouragement is given or when the encouragement is infrequent (i.e. every 180 s).     

Changes in running economy following downhill running

In this study, we examined the time course of changes in running economy following a 30-min downhill (-15%) run at 70% peak aerobic power (VO2peak). Ten young men performed level running at 65, 75, and 85% VO2peak (5 min for each intensity) before, immediately after, and 1 - 5 days after the downhill run, at which times oxygen consumption (VO2), minute ventilation, the respiratory exchange ratio (RER), heart rate, ratings of perceived exertion (RPE), and blood lactate concentration were measured. Stride length, stride frequency, and range of motion of the ankle, knee, and hip joints during the level runs were analysed using high-speed (120-Hz) video images. Downhill running induced reductions (7 - 21%, P < 0.05) in maximal isometric strength of the knee extensors, three- to six-fold increases in plasma creatine kinase activity and myoglobin concentration, and muscle soreness for 4 days after the downhill run. Oxygen consumption increased (4 - 7%, P < 0.05) immediately to 3 days after downhill running. There were also increases (P < 0.05) in heart rate, minute ventilation, RER, RPE, blood lactate concentration, and stride frequency, as well as reductions in stride length and range of motion of the ankle and knee. The results suggest that changes in running form and compromised muscle function due to muscle damage contribute to the reduction in running economy for 3 days after downhill running.     

Effects of nicotinamide adenine dinucleotide hydride on physical and mental performance

The aim of this study was to assess the effects of nicotinamide adenine dinucleotide hydride (NADH) on maximal oxygen uptake (VO2max), maximal anaerobic running time, and mental performance. Eight men were exposed to a supplement treatment (30 mg NADH as ENACHI tablets per day) and to a placebo treatment, each of 4 weeks' duration, in a balanced, double-blind, and cross-over design. The two treatments were separated by a 14-week wash-out period. The results indicated that VO2max, maximal anaerobic running time, and the ability to concentrate were similar in the NADH and placebo conditions. There were also no differences in blood lactate, creatine kinase, reaction time or feelings of fatigue between the treatments. A counter-movement jump performed at rest and 2 min after the aerobic test differed significantly (P 相似文献   

Effect of age on 16.1-km time-trial performance

In this study, we assessed the performance of trained senior (n = 6) and veteran (n = 6) cyclists (mean age 28 years, s = 3 and 57 years, s = 4 respectively). Each competitor completed two cycling tests, a ramped peak aerobic test and an indoor 16.1-km time-trial. The tests were performed using a Kingcycle ergometer with the cyclists riding their own bicycle fitted with an SRM powermeter. Power output, heart rate, and gas exchange variables were recorded continuously and blood lactate concentration [HLa] was assessed 3 min after the peak ramped test and at 2.5-min intervals during the time-trial. Peak values for power output (RMP(max)), heart rate (HR(peak)), oxygen uptake (VO2(peak)), and ventilation (V(Epeak)) attained during the ramped test were higher in the senior group (P < 0.05), whereas [HLa](peak), RER(peak), V(E): VO2(peak), and economy(peak) were similar between groups (P > 0.05). Time-trial values (mean for duration of race) for power output (W(TT)), heart rate (HR(TT)), VO2 (VO(2TT)), and V(E) (V(ETT)) were higher in the seniors (P < 0.05), but [HLa](TT), RER(TT), V(ETT): VO2(TT), and economy(TT) were similar between the groups (P > 0.05). Time-trial exercise intensity, expressed as %RMP(max), %HR(peak), % VO2(peak), and % V(Epeak), was similar (P > 0.05) for seniors and veterans (W(TT): 81%, s = 2 vs. 78%, s = 8; HR(TT): 96%, s = 4 vs. 94%, s = 4; VO2(TT): 92%, s = 4 vs. 95%, s = 10; V(ETT): 89%, s = 8 vs. 85%, s = 8, respectively). Overall, seniors attained higher absolute values for power output, heart rate, VO2, and V(E) but not blood lactate concentration, respiratory exchange ratio (RER), V(E): VO2, and economy. Veterans did not accommodate age-related declines in time trial performance by maintaining higher relative exercise intensity.     

The relationship between selected physiological variables of rowers and rowing performance as determined by a 2000 m ergometer test

The aim of this study was to establish the relationship between selected physiological variables of rowers and rowing performance as determined by a 2000 m time-trial on a Concept II Model B rowing ergometer. The participants were 13 male club standard oarsmen. Their mean (+/- s) age, body mass and height were 19.9+/-0.6 years, 73.1+/-6.6 kg and 180.5+/-4.6 cm respectively. The participants were tested on the rowing ergometer to determine their maximal oxygen uptake (VO2max), rowing economy, predicted velocity at VO2max, velocity and VO2 at the lactate threshold, and their velocity and VO2 at a blood lactate concentration of 4 mmol x l(-1). Percent body fat was estimated using the skinfold method. The velocity for the 2000 m performance test and the predicted velocities at the lactate threshold, at a blood lactate concentration of 4 mmol x l(-1) and at VO2max were 4.7+/-0.2, 3.9+/-0.2, 4.2+/-0.2 and 4.6+/-0.2 m x s(-1) respectively. A repeated-measures analysis of variance showed that the three predicted velocities were all significantly different from each other (P<0.05). The VO2max and lean body mass showed the highest correlation with the velocity for the 2000 m time-trial (r = 0.85). A stepwise multiple regression showed that VO2max was the best single predictor of the velocity for the 2000 m time-trial; a model incorporating VO2max explained 72% of the variability in 2000 m rowing performance. Our results suggest that rowers should devote time to the improvement of VO2max and lean body mass.     

Carbohydrate and carbohydrate + protein for cycling time-trial performance

Carbohydrate intake during endurance exercise delays the onset of fatigue and improves performance. Two recent cycling studies have reported increased time to exhaustion when protein is ingested together with carbohydrate. The purpose of the present study was to test the hypothesis that ingestion of a carbohydrate + protein beverage will lead to significant improvements in cycling time-trial performance relative to placebo and carbohydrate alone. Thirteen cyclists completed 120 min of constant-load ergometer cycling. Thereafter, participants performed a time-trial in which they completed a set amount of work (7 kJ kg(-1)) as quickly as possible. Participants completed four experimental trials, the first for familiarization and then three randomized, double-blind treatments consisting of a placebo, carbohydrate, and carbohydrate + protein. Participants received 250 ml of beverage every 15 min during the constant-load ride. Time-trial performance for carbohydrate (37.1 min, s = 3.8) was significantly (P < 0.05) faster than placebo (39.7 min, s = 4.6). Time-trial performance for carbohydrate + protein (38.8 min, s = 5.5) was not significantly different from either placebo or carbohydrate. Ingestion of a carbohydrate beverage during two hours of constant-load cycling significantly enhanced subsequent time-trial performance compared with placebo. The carbohydrate + protein beverage provided no additional performance benefit.     

Effect of glucose polymer ingestion on energy and fluid balance during exercise

Nine male triathletes were studied during 160 min of exercise at 65% VO2 max on two occasions to examine the effect of glucose polymer ingestion on energy and fluid balance. During one trial they received 200 ml of a 10% glucose polymer solution at 20 min intervals during exercise (CHO), while in the other they received an equal volume of a sweet placebo (CON). On average, blood glucose levels (CON = 4.2 +/- 0.2 mmol l-1, CHO = 4.8 +/- 0.1, mean +/- S.E.) and respiratory exchange ratios (CON = 0.84 +/- 0.01, CHO = 0.87 +/- 0.01) during exercise were higher (P less than 0.05) as a result of the glucose polymer ingestion. There were no differences between trials, however, in the estimated plasma volume changes during exercise. Exercise time to exhaustion at an intensity corresponding to 110% VO2 max, performed 5 min after the submaximal exercise, was not influenced by glucose polymer ingestion. Relative to a control exercise bout conducted without prior exercise, however, sprint performance and postexercise blood lactate accumulation were impaired in both trials. It is concluded that glucose polymer ingestion maintains blood glucose levels and a high rate of carbohydrate oxidation during prolonged exercise, without compromising fluid balance.     

Prediction of maximal oxygen uptake in sedentary males from a perceptually regulated, sub-maximal graded exercise test

The purpose of this study was to assess the validity of predicting the maximal oxygen uptake (VO2(max)) of sedentary men from sub-maximal VO2 values obtained during a perceptually regulated exercise test. Thirteen healthy, sedentary males aged 29-52 years completed five graded exercise tests on a cycle ergometer. The first and fifth test involved a graded exercise test to determine VO2(max). The two maximal graded exercise tests were separated by three sub-maximal graded exercise tests, perceptually regulated at 3-min RPE intensities of 9, 11, 13, 15, and 17 on the Borg ratings of perceived exertion (RPE) scale, in that order. After confirmation that individual linear regression models provided the most appropriate fit to the data, the regression lines for the perceptual ranges 9-17, 9-15, and 11-17 were extrapolated to RPE 20 to predict VO2(max). There were no significant differences between VO2(max) values from the graded exercise tests (mean 43.9 ml x kg(-1) x min(-1), s = 6.3) and predicted VO2(max) values for the perceptual ranges 9-17 (40.7 ml x kg(-1) x min(-1), s = 2.2) and RPE 11-17 (42.5 ml x kg(-1) x min(-1), s = 2.3) across the three trials. The predicted VO2(max) from the perceptual range 9-15 was significantly lower (P < 0.05) (37.7 ml x kg(-1) x min(-1), s = 2.3). The intra-class correlation coefficients between actual and predicted VO2(max) for RPE 9-17 and RPE 11-17 across trials ranged from 0.80 to 0.87. Limits of agreement analysis on actual and predicted VO2 values (bias +/- 1.96 x S(diff)) were 3.4 ml x kg(-1) x min(-1) (+/- 10.7), 2.4 ml x kg(-1) x min(-1) (+/- 9.9), and 3.7 ml x kg(-1) x min(-1) (+/- 12.8) (trials 1, 2, and 3, respectively) of RPE range 9-17. Results suggest that a sub-maximal, perceptually guided graded exercise test provides acceptable estimates of VO2(max) in young to middle-aged sedentary males.     

Criterion-related validity of the Borg ratings of perceived exertion scale in healthy individuals: a meta-analysis

The literature related to Borg's ratings of perceived exertion (RPE) scale has revealed inconsistencies about the strength of the relationship between ratings of perceived exertion and various physiological criterion measures, such as heart rate, blood lactate concentration, percent maximal oxygen uptake (%VO2max), oxygen uptake (VO2), ventilation and respiration rate. Using sex of participants, fitness, type of RPE scale used, type of exercise, exercise protocol, RPE mode and study quality, we undertook a meta-analysis to determine the strength of the relationship between RPE scores and the six aforementioned physiological measures. The weighted mean validity coefficients were 0.62 for heart rate, 0.57 for blood lactate, 0.64 for %VO2max 0.63 for VO2, 0.61 for ventilation and 0.72 for respiration rate. Analysis of moderator variables revealed that the following study features could account for the variation of results across studies: heart rate--fitness, type of exercise, protocol and RPE mode; blood lactate concentration--sex, RPE scale; VO2--sex, exercise type, RPE mode; ventilation--sex, RPE mode; respiration rate--exercise protocol, RPE mode. The highest correlations between ratings of perceived exertion and the various physiological criterion measures were found in the following conditions: when male participants (whose VO2 or ventilation was measured) were required to maximally exert themselves (measuring %VO2max or ventilation); when the exercise task was unusual [e.g. when participants were swimming, which is less common than walking or running (when heart rate, %VO2max and VO2 are measured)]; or when the 15-point RPE scale (measuring blood lactate concentration) was used. These findings suggest that although Borg's RPE scale has been shown to be a valid measure of exercise intensity, its validity may not be as high as previously thought (r = 0.80-0.90), except under certain conditions.     

Physiological and metabolic responses of men and women to a 5-km treadmill time trial.

Previous studies have reported strong correlations between 5-km performance times and maximal oxygen uptake (VO2 max) and also for running speeds equivalent to blood lactate concentrations of 4 mM. However, there is little information on the physiological responses of individuals during races over this distance. Therefore, the aim of the present study was to measure the physiological and metabolic responses of endurance trained male (n = 8) and female (n = 8) runners during a 5-km time trial using an instrumented treadmill. Performance times were 18.77 +/- 1.27 min for the men and 21.80 +/- 1.98 min for the women (P less than 0.01). The corresponding times on the athletics track were 17.68 +/- 0.39 min for the men (P less than 0.05) and 20.70 +/- 2.16 min for the women (N.S.). During the treadmill time trials, both the men and women were able to utilize approximately 90% VO2 max, 82% VE max, 98% HR max and produce similar concentrations of blood lactate. Although the physiological and metabolic responses of these endurance-trained men and women to 5-km treadmill running were similar, the faster running times recorded by the men in this study were the result of their higher VO2 max values.     

No effect of glutamine supplementation and hyperoxia on oxidative metabolism and performance during high-intensity exercise

Glutamine enhances the exercise-induced expansion of the tricarboxylic acid intermediate pool. The aim of the present study was to determine whether oral glutamine, alone or in combination with hyperoxia, influenced oxidative metabolism and cycle time-trial performance. Eight participants consumed either placebo or 0.125 g kg body mass(-1) of glutamine in 5 ml kg body mass(-1) placebo 1 h before exercise in normoxic (control and glutamine respectively) or hyperoxic (FiO(2) = 50%; hyperoxia and hyperoxia + glutamine respectively) conditions. Participants then cycled for 6 min at 70% maximal oxygen uptake (VO(2max)) immediately before completing a brief high-intensity time-trial (approximately 4 min) during which a pre-determined volume of work was completed as fast as possible. The increment in pulmonary oxygen uptake during the performance test (DeltaVO(2max), P = 0.02) and exercise performance (control: 243 s, s(x) = 7; glutamine: 242 s, s(x) = 3; hyperoxia: 231 s, s(x) = 3; hyperoxia + glutamine: 228 s, s(x) = 5; P < 0.01) were significantly improved in hyperoxic conditions. There was some evidence that glutamine ingestion increased DeltaVO(2max) in normoxia, but not hyperoxia (interaction drink/FiO(2), P = 0.04), but there was no main effect or impact on performance. Overall, the data show no effect of glutamine ingestion either alone or in combination with hyperoxia, and thus no limiting effect of the tricarboxylic acid intermediate pool size, on oxidative metabolism and performance during maximal exercise.     

The effects of frequency of encouragement on performance during maximal exercise testing

The aim of this study was to determine the effects of frequency of verbal encouragement during maximal exercise testing. Twenty-eight participants (12 males, 16 females) aged 20.9 - 1.5 years (mean - s ) performed a maximal exercise test ( V O 2max ) on a treadmill without any verbal encouragement. The participants were matched according to their pre-test V O 2max and placed into either a control group or one of three experimental groups. They performed a second exercise test (post-test) 1 week later. During the second test, the control group received no verbal encouragement; the 20 s (20E), 60 s (60E) and 180 s (180E) encouragement groups received verbal encouragement every 20, 60 and 180 s, respectively, beginning with stage 3 of the exercise test. Relative V O 2max , exercise time, blood lactate concentration, respiratory exchange ratio (RER) and ratings of perceived exertion (RPE) were not significantly different from the first test to the second test for the control group without verbal encouragement and the 180E group that received infrequent encouragement. Post-test values were significantly higher than pre-test values for the 20E and 60E groups. The post-test values of the 20E group were significantly higher than their pre-test values for relative V O 2max ( P ? 0.001), exercise time ( P ? 0.0001), blood lactate concentr . ation ( P ? 0.05), RER ( P ? 0.01) and RPE ( P ? 0.0001); this was also the case for the 60E group for relative V O 2max ( P ? 0.01), blood lactate concentration ( P ? 0.05), RER ( P ? 0.05) and RPE ( P ? 0.05). The results suggest that frequent verbal encouragement (every 20 s and 60 s in the present study) leads to significantly greater maximum effort in a treadmill test than when no encouragement is given or when the encouragement is infrequent (i.e. every 180 s).     

Sodium bicarbonate ingestion does not alter the slow component of oxygen uptake kinetics in professional cyclists

We examined the effects of pre-exercise sodium bicarbonate (NaHCO3) ingestion on the slow component of oxygen uptake (VO2) kinetics in seven professional road cyclists during intense exercise. One hour after ingesting either a placebo or NaHCO3 (0.3 g x kg body mass(-1)), each cyclist (age, 25 +/- 2 years; VO2max, 74.7 +/- 5.9 ml x kg(-1) x min(-1); mean +/- s) performed two bouts of 6 min duration at an intensity of 90% VO2max interspersed by 8 min of active recovery. Gas exchange and blood data (pH, blood lactate concentration and [HCO3-]) were collected during the tests. In both bouts, the slow component of VO2 was defined as the difference between end-exercise VO2 and the VO2 at the end of the third minute. No significant difference was found in the slow component of VO2 between conditions in the first (NaHCO3, 210 +/- 69 ml; placebo, 239 +/- 105 ml) or second trial (NaHCO3, 123 +/- 88 ml; placebo, 197 +/- 101 ml). In conclusion, pre-exercise NaHCO3 ingestion did not significantly attenuate the VO2 slow component of professional road cyclists during high-intensity exercise.     

Effect of 6 weeks of endurance training on the lactate minimum speed

The aim of this study was to assess the sensitivity of the lactate minimum speed test to changes in endurance fitness resulting from a 6 week training intervention. Sixteen participants (mean +/- s: age 23+/-4 years; body mass 69.7+/-9.1 kg) completed 6 weeks of endurance training. Another eight participants (age 23+/-4 years; body mass 72.7+/-12.5 kg) acted as non-training controls. Before and after the training intervention, all participants completed: (1) a standard multi-stage treadmill test for the assessment of VO2max, running speed at the lactate threshold and running speed at a reference blood lactate concentration of 3 mmol x l(-1); and (2) the lactate minimum speed test, which involved two supramaximal exercise bouts and an 8 min walking recovery period to increase blood lactate concentration before the completion of an incremental treadmill test. Additionally, a subgroup of eight participants from the training intervention completed a series of constant-speed runs for determination of running speed at the maximal lactate steady state. The test protocols were identical before and after the 6 week intervention. The control group showed no significant changes in VO2max, running speed at the lactate threshold, running speed at a blood lactate concentration of 3 mmol x l(-1) or the lactate minimum speed. In the training group, there was a significant increase in VO2max (from 47.9+/-8.4 to 52.2+/-2.7 ml x kg(-1) x min(-1)), running speed at the maximal lactate steady state (from 13.3+/-1.7 to 13.9+/-1.6 km x h(-1)), running speed at the lactate threshold (from 11.2+/-1.8 to 11.9+/-1.8 km x h(-1)) and running speed at a blood lactate concentration of 3 mmol x l(-1) (from 12.5+/-2.2 to 13.2+/-2.1 km x h(-1)) (all P < 0.05). Despite these clear improvements in aerobic fitness, there was no significant difference in lactate minimum speed after the training intervention (from 11.0+/-0.7 to 10.9+/-1.7 km x h(-1)). The results demonstrate that the lactate minimum speed, when assessed using the same exercise protocol before and after 6 weeks of aerobic exercise training, is not sensitive to changes in endurance capacity.     

Effect of 6 weeks of endurance training on the lactate minimum speed

The aim of this study was to assess the sensitivity of the lactate minimum speed test to changes in endurance fitness resulting from a 6 week training intervention. Sixteen participants (mean +/- s :age 23 +/- 4 years;body mass 69.7 +/- 9.1 kg) completed 6 weeks of endurance training. Another eight participants (age 23 +/- 4 years; body mass 72.7 +/-12.5 kg) acted as non-training controls. Before and after the training intervention, all participants completed: (1) a standard multi-stage treadmill test for the assessment of VO 2max , running speed at the lactate threshold and running speed at a reference blood lactate concentration of 3 mmol.l -1 ; and (2) the lactate minimum speed test, which involved two supramaximal exercise bouts and an 8 min walking recovery period to increase blood lactate concentration before the completion of an incremental treadmill test. Additionally, a subgroup of eight participants from the training intervention completed a series of constant-speed runs for determination of running speed at the maximal lactate steady state. The test protocols were identical before and after the 6 week intervention. The control group showed no significant changes in VO 2max , running speed at the lactate threshold, running speed at a blood lactate concentration of 3 mmol.l -1 or the lactate minimum speed.In the training group, there was a significant increase in VO 2max (from 47.9 +/- 8.4 to 52.2 +/- 2.7 ml.kg -1 .min -1 ), running speed at the maximal lactate steady state (from 13.3 +/- 1.7 to 13.9 +/- 1.6 km.h -1 ), running speed at the lactate threshold (from 11.2 +/- 1.8 to 11.9 +/- 1.8 km.h -1 ) and running speed at a blood lactate concentration of 3 mmol.l -1 (from 12.5 +/- 2.2 to 13.2 +/- 2.1 km.h -1 ) (all P ? 0.05). Despite these clear improvements in aerobic fitness, there was no significant difference in lactate minimum speed after the training intervention (from 11.0 +/- 0.7 to 10.9 +/- 1.7 km.h -1 ). The results demonstrate that the lactate minimum speed,when assessed using the same exercise protocol before and after 6 weeks of aerobic exercise training, is not sensitive to changes in endurance capacity.     

The effect of caffeine ingestion on human neutrophil oxidative burst responses following time-trial cycling

Following fixed-duration exercise of submaximal intensity, caffeine ingestion is associated with an attenuation of the exercise-induced decline in N-formyl-methionyl-phenyl-alanine (f-MLP) stimulated neutrophil oxidative burst. However, the response following high-intensity exhaustive exercise is unknown. Nine endurance-trained male cyclists ingested 6 mg caffeine or placebo per kilogram of body mass 60 min before cycling for 90 min at 70% of maximal oxygen consumption (VO2max) and then performing a time-trial requiring an energy expenditure equivalent to 30 min cycling at 70% maximum power output. Time-trial performance was 4% faster in the caffeine than in the placebo trial (P = 0.043). Caffeine was associated with an increased plasma adrenaline concentration after 90 min of exercise (P = 0.046) and immediately after the time-trial (P = 0.02). Caffeine was also associated with an increased serum caffeine concentration (P < 0.01) after 90 min of exercise and immediately after the time-trial, as well as 1 h after the time-trial. However, the f-MLP-stimulated neutrophil oxidative burst response fell after exercise in both trials (P = 0.002). There was no effect of caffeine on circulating leukocyte or neutrophil counts, but the lymphocyte count was significantly lower on caffeine (20%) after the time-trial (P = 0.003). Our results suggest that high-intensity exhaustive exercise negates the attenuation of the exercise-induced decrease in neutrophil oxidative burst responses previously observed when caffeine is ingested before exercise of fixed duration and intensity. This may be associated with the greater increase in adrenaline concentration observed in the present study.     

The effects of carbohydrate supplementation during repeated bouts of prolonged exercise on saliva flow rate and immunoglobulin A

The purpose of this study was to assess the effect of carbohydrate (CHO) feeding during different periods of two 90-min cycling bouts (the first bout began at 09:00?h and the second bout began at 13:30 h) at 60% maximal oxygen uptake(VO2max) on saliva flow rate and saliva immunoglobulin A (sIgA) responses to the second exercise bout. The study consisted of three investigations: carbohydrate supplementation during (1) the first hour of the recovery interval (CHO-REC), (2) during the first bout of exercise and (3) during the second bout of exercise. Each investigation included two trials completed in a counterbalanced order and separated by at least 4 days. Participants consumed a lemon-flavoured 10% w/v carbohydrate beverage or placebo (22 ml.kg-1 body mass) in the first hour of the recovery interval (n=8) and 500 ml just before exercise, followed by 250 ml every 20 min during exercise in the first (n=9) and second exercise bouts (n=9). Timed unstimulated saliva samples were collected at 10 min before exercise, after 48-50 min of exercise and during the last 2 min of exercise, at 1 h post exercise, 2 h post exercise (first exercise bout only), and 18 h post exercise (second exercise bout only). Venous blood samples were taken 5 min before exercise and immediately after exercise for both exercise bouts in all trials. The main findings of the present study were as follows. First, carbohydrate ingestion during both exercise bouts, but not during the recovery interval, better maintained plasma glucose concentrations and attenuated the increase in plasma adrenaline and cortisol concentrations after the second exercise bout compared with placebo. Second, carbohydrate feeding had no effect on saliva flow rate and sIgA secretion rate compared with placebo. Third, saliva flow rate and sIgA concentration returned to pre-exercise bout 1 values within 2 h in all trials. Fourth, there was no delayed effect of exercise on oral immunity. These findings suggest that carbohydrate ingestion during the first or second bout of exercise, but not during the recovery interval, is likely to better maintain plasma glucose concentrations and attenuate the responses of plasma stress hormones to a second exercise bout than ingestion of fluid alone. Two bouts of 90 min cycling at 60% VO2max on the same day appears to inhibit saliva flow rate during the second exercise bout but does not alter sIgA transcytosis. Our results show that carbohydrate ingestion during any period of two prolonged exercise bouts does not induce different effects on oral immunity compared with placebo.     

Endurance running performance in athletes with asthma

Laboratory assessment was made during maximal and submaximal exercise on 16 endurance trained male runners with asthma (aged 35 +/- 9 years) (mean +/- S.D.). Eleven of these asthmatic athletes had recent performance times over a half-marathon, which were examined in light of the results from the laboratory tests. The maximum oxygen uptake (VO2max) of the group was 61.8 +/- 6.3 ml kg-1 min-1 and the maximum ventilation (VEmax) was 138.7 +/- 24.7 l min-1. These maximum cardio-respiratory responses to exercise were positively correlated to the degree of airflow obstruction, defined as the forced expiratory volume in 1 s (expressed as a percentage of predicted normal). The half-marathon performance times of 11 of the athletes ranged from those of recreational to elite runners (82.4 +/- 8.8 min, range 69-94). Race pace was correlated with VO2max (r = 0.863, P less than 0.01) but the highest correlation was with the running velocity at a blood lactate concentration of 2 mmol l-1 (r = 0.971, P less than 0.01). The asthmatic athletes utilized 82 +/- 4% VO2max during the half-marathon, which was correlated with the %VO2max at 2 mmol l-1 blood lactate (r = 0.817, P less than 0.01). The results of this study suggest that athletes with mild to moderate asthma can possess high VO2max values and can develop a high degree of endurance fitness, as defined by their ability to sustain a high percentage of VO2max over an endurance race. In athletes with more severe airflow obstruction, the maximum ventilation rate may be reduced and so VO2max may be impaired. The athletes in the present study have adapted to this limitation by being able to sustain a higher %VO2max before the accumulation of blood lactate, which is an advantage during an endurance race. Therefore, with appropriate training and medication, asthmatics can successfully participate in endurance running at a competitive level.