Co^2＋、Ni^2＋、Cd^2＋对过氧化氢酶动力学的影响

在生理条件下，本实验探索了Co^2 、Ni^2 、Cd^2 对过氧化氢稳定性和过氧化氢酶生物活性的影响。结果表明，Co^2 、Ni^2 、Cd^2 对过氧化氢的分解反应具有正催化作用，过氧化氢酶的生物活性均有可逆的抑制作用，其抑制能力的大小顺序为Co^2 ＞Cd^2＋＞Ni^2 。     

一株互花米草耐重金属内生菌的分离及其特性分析

利用微生物分离培养方法从互花米草根部和叶片获得内生细菌,经重金属筛选,分离到一种耐受重金属Cu2＋、Pb2＋和Cr6＋的细菌．为了进一步鉴定分离菌株,利用 PCR 方法扩增其部分16S rDNA 序列,目标DNA条带约1500 bp,测序和在线分析结果后显示,所测序列均与基因库中已上传的 Lysinibacillus sphaericus菌株16S rDNA 序列（KJ878614．1,JN613478．1）相似性均为100％．初步鉴定该菌为 Lysinibacillus属球形芽孢杆菌（Lysinibacillus sphaericus）,命名为Lysinibacillus sphaericus QZ1-1．对该菌株采用促生长特性分析结果显示,该菌株具有产生 IAA和 ACC的能力,同时该菌能在无氮培养基中生长,具备固氮功能,为日后重金属污染生物修复相关研究提供了良好的目标菌株．     

大葱叶际生防细菌筛选及对紫斑病防效研究

为了挖掘大葱紫斑病生防资源,研究采用培养法从大葱叶际分离细菌,然后用对峙培养法筛选大葱紫斑病菌生防细菌.结果表明:大葱叶际细菌群落中嗜甲基单胞菌、鞘氨醇单胞菌属、微杆菌属、假单胞菌属于优势细菌类群.从中筛选出1株对大葱紫斑病菌具有较好拮抗活性的菌株X85,经形态特征和16S rDNA序列鉴定为亚麻假单胞菌.田间试验表明...     

钾细菌的分离及对Cu^2＋的耐受性实验研究

利用分离培养基从草坪、芦苇地、公园以及菜园土壤中分离到一株高效钾细菌菌株,经纯化后,研究了钾细菌对铜的耐受阈值,并筛选了提高钾细菌Cu^2＋耐受阈的钝化剂。结果表明,钾细菌最高能耐受Cu^2＋的浓度为50mg/L。投加钝化剂粉煤灰和石灰后,钾细菌对Cu^2＋的耐受阈提高到80mg/L,粉煤灰对重金属铜的钝化性能优于石灰。     

铜、铁、钴、镍纸上层析分离的显色剂研究

高师化学专业分析化学实验中有“铜、铁、钴、镍的纸上层析分离法”的实验，但所需显色剂二硫代乙二酰胺长期缺货，使该实验难以开出，笔者研究了替代显色剂及有关实验条件，发现用K4Fe(CN)6 K3Fe(CN)6混合液作显色剂，可保证Cu^2 、Fe^3 、Co^2 离子的色斑清楚显示，但显色剂造成的黄绿色背景使Ni^2 色斑辨认困难．若用K4Fe(CN)6 K3Fe(CN)6 丁二酮肟混合液显色，然后用浓氨水薰，则Ni^2 可形成清晰的红色斑点．使用这两种显色剂时，层析滤纸事先不在展开剂中饱和，分离效果更好；浓氨水也不宜在喷洒显色剂之前使用．     

水溶性新显色剂5—NO2—PADN—S的制备与分析特性的初步探讨

报道了水溶性新显色剂1－（5－硝基－2－吡啶偶氮－2，7－萘二酚－6磺酸（5－NO2－PADN－S）的制备方法；并初步探讨了5－NO2－PADN－S的性质及其与金属离子显色反应的光度特性。结果表明，该试剂在pH6．2的HAc-NaAc缓冲溶液中可与Fe^2 、Co^2＋、Ni^2 、Pd^2 等金属离子在水溶液中直接发生显色反应，生成稳定的有色配合物。     

关白附内生菌的分离鉴定及其抗菌和细胞毒活性研究

从关白附（Aconitum Coreanum（Levl.）Rapaics）中分离出内生菌共29株,通过TLC检测筛选出2株可能产生生物碱类成分的内生菌.对筛选出的2株内生菌株的发酵培养物分别以纸片法和MTT法进行抑菌和细胞毒活性测定.实验结果显示,2个内生菌株发酵产物对金黄色葡萄球菌、大肠杆菌具有良好的抑菌作用,对肝癌细胞SMMC-7721、胃癌细胞SGC-7901具有良好的细胞毒活性.通过16S rDNA基因序列分析,2株内生菌分别被鉴定为类芽孢杆菌属和芽孢杆菌属细菌.     

6种海洋微藻提取物抑菌活性研究

对实验室培养的6种海洋微藻用有机溶剂与水提取其活性物质,提取物用圆形纸片法对3种细菌和2种真菌的生长进行了抑制实验.结果表明:在6种海洋微藻中,塔胞藻的抗菌谱最广,分别对4种菌的生长有抑制作用.亚心形扁藻与塔胞藻的提取物对枯草芽孢杆菌的抗性最强.在所有的菌中,黑曲霉对微藻提取物较敏感.不同的微藻对革兰氏阳性菌和革兰氏阴性菌有不同的抑制作用.微藻对细菌的抑制作用大于对真菌的抑制作用.     

镍胁迫对大豆种子萌发过程中的生理生化影响

以两个大豆品种（周豆11号和豫豆24号）为材料，研究了不同浓度Ni^2＋处理对大豆种子萌发和幼苗生长中膜脂过氧化水平、细胞膜透性及保护酶（SOD、POD、CAT）活性的影响．结果表明，（1）Ni^2＋浸种处理对大豆种子萌发具有低浓度下的激活效应和高浓度下的抑制效应；（2）细胞内丙二醛（MDA）含量和细胞膜透性在低浓度Ni^2＋处理下略有降低，尔后随着镍浓度的提高其质膜透性和MDA含量逐渐增大，体内保护酶系统中超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性的变化表现为先升后降的趋势．不同大豆品种对NP的反应存在霉鼻与抗氢化系统的整体活力有关．     

楚雄州中医院2011年细菌耐药性监测结果分析

目的：了解2011年临床分离菌株对常用抗生素的耐药性。方法：采用API鉴定和ATB药敏板条及纸片扩散法对2011年临床分离1127株细菌进行药敏试验。结果：1127株细菌中革兰阴性菌占76．8％,革兰阳性菌占23．2％。革兰阴性菌中前3位为大肠埃希菌45．7％（395／865）、肺炎克雷伯菌10．6％（92／865）和铜绿假单胞菌9．0％（78／865）;革兰阳性菌中前3位为屎肠球菌27．1％（71／262）、金黄色葡萄球菌24．8％（65／262）和表皮葡萄球菌16．0％（42／262）。药敏试验结果显示泛耐药铜绿假单胞菌和不动杆菌检出率分别为19．2％（15／78）和32．8％（23／70）;MRSA和MRCNS分别占金黄色葡萄球菌和CNS的32．3％（21／65）和83．3％（40／48）。结论：定期进行耐药性监测有助于了解细菌耐药性的变迁,为临床用药提供依据。     

卡思卡特城堡

It is worthy of noting that, whilst Crookston Castle witnessed the earlier and happier portion of Mary's variegated life,     

不同的吃喝玩乐

一、吃和喝吃苹果 eat an apple, 吃药 take medicine,吃糖 have some sweets,吃饭 have one's meals,吃馆子 dine out,吃惊 be surprised/     

漏洞百出的教育

Many college freshmen arrive woefully unprepared to do college work, and as disadvantaged populations continue to grow, the share of the American work force that has made it through college is expected to plummet. Many experts blame that educational failure not just on high schools but also on colleges. School & College, a special report by The Chronicle, looks at efforts to fix the system. What reforms would better prepare students for college? What should schools and colleges be doing differently? How should state and federal officials help?     

汉字倒说

汉字的六书,《说文》对“转注”一类,语焉不详。后世学者提及转注,也仅限于许慎所举出的例字。《汉字例说》一文,作者从转注的角度综合考虑,对部分现代常用字作了分析,跟传统的解释有所不同。希望能抛砖引玉,互相切磋,以推进学术研究。     

只要努力,不要后悔

There are numbers of crossroads on our long and unpredictable life journey where we totally have no idea about which direction to choose. No matter what our decision is, we should not turn back, but face the music and go ahead instead. I am this kind of girl who always does try without regretting, one example is how I dealt with my love.     

浅谈移民问题

Migration occurs behind a variety of reasons and has a great effect on the whole world. People may migrate in order to improve their economic situation, or in order to escape civil strife, persecution, and environmental disasters. The impact of migration is complex, bringing both benefits anddisadvantages. This paper briefly talks about the causes of migration, the allocation of benefits, and the ways in which individual countries and the international community deal with this important subject.     

钱生钱!

我们绝大部分人都同意一件事情——我们喜欢金钱。但是你对金钱的喜欢之情足够到让你创立一份事业么?.财富通常被人忽视,主要在于人们不能真正了     

Sister Carrie, an Adherent of Desires

Sister Carrie is one of the most controversial characters in American literature.Thought as a "fallen woman" firstly,she was defined as a "new woman" by some critics later. However, by digging into the motivaton behind the whole process of Carrie's "success", the relationship between Carrie and her creator (the author), the social conditions of then American, it can be found that Carrie has never been free-standing on her thought and she has never found her real-sdf even after becoming a famous actress. In a society dominated by mass consumerism Carrie is only an adherent of her own desires. She also is a representative of all those country girls flooded into cities, a symbol and a sacrifice of the urbanization of America in a time countryside was overcome by cities.     

Improvement of one-cycle controller by use of proportional integral differential controller

1.IntroductionOne-cyclecontrolmethod,whichwasproposedaboutonedecadeago[1],hasbecomeanattractivemethodinspecialfieldssuchaspowerfactorcorrection[2-6],switchingamplifiers[7,8],etc.Themainideaofthiscontrollerisbasedonintegrationofdiodevoltageinone-cycleandforcesittobeexactlyequaltothereferencevalue.Themainadvantageofthiscontrollerisitsrealtimeabilitytorejectthevariationofinputvoltage[1].Despitethisgreatability,ithasnogoodperformancesinrejectingofloaddisturbanceandfollowingreferencecommands.Espec…