A microfluidic device for on-chip agarose microbead generation with ultralow reagent consumption

Water-in-oil microdroplets offer microreactors for compartmentalized biochemical reactions with high throughput. Recently, the combination with a sol-gel switch ability, using agarose-in-oil microdroplets, has increased the range of possible applications, allowing for example the capture of amplicons in the gel phase for the preservation of monoclonality during a PCR reaction. Here, we report a new method for generating such agarose-in-oil microdroplets on a microfluidic device, with minimized inlet dead volume, on-chip cooling, and in situ monitoring of biochemical reactions within the gelified microbeads. We used a flow-focusing microchannel network and successfully generated agarose microdroplets at room temperature using the “push-pull” method. This method consists in pushing the oil continuous phase only, while suction is applied to the device outlet. The agarose phase present at the inlet is thus aspirated in the device, and segmented in microdroplets. The cooling system consists of two copper wires embedded in the microfluidic device. The transition from agarose microdroplets to microbeads provides additional stability and facilitated manipulation. We demonstrate the potential of this method by performing on-chip a temperature-triggered DNA isothermal amplification in agarose microbeads. Our device thus provides a new way to generate microbeads with high throughput and no dead volume for biochemical applications.     

Grades degrade group coordination: deteriorated interactions and performance in a cooperative motor task

At school, pupils often cooperate on common projects and must coordinate their different individual actions. However, grades are pervasively used even in cooperative situations, which make the pupils’ differences in achievement and their relative rank salient and may reduce their inclination to work constructively with others. Thus, we hypothesized that grades would elicit disruptive interactions and reduce performance in a cooperative cognitive-motor task necessitating inter-individual coordination of members. In a study with 5th graders, grades (vs. a neutral concept) were primed at the onset of a cooperative group interaction. Results showed that, although pupils were set to work cooperatively, priming grades (vs. neutral concepts) harmed inter-individual coordination by eliciting more negative dominant behaviours among pupils during interactions, which decreased group performance.     

France: The National Centre for Distance Teaching

University Professor, Chief Education Officer and Director of the Centre National d'Enseignement à Distance (CNED) from December 1985 to December 1987. Author of several works on epistemology, history of science, social problems.     

L’apprentissage précoce des langues : Des pratiques sociologiquement et politiquement marquées

Throughout the world there is a great diversity of curricula for introducing early foreign language training more or less intensively. These curricula correspond to different pedagogical approaches. But these pedagogical choices are not neutral. They are the result of political choice. They are also sociologically discriminatory. The author studies and contrasts various forms of language training of certain countries and within the same countries (for example, the correlation between bilingual training and private schools, or the choice of bilingual education as a political goal). She proposes a scale for classifying the kinds of early foreign language training, depending on sociological criteria and political choice.     

The Design of Competency-Based Performance Assessment in E-Learning

This article focuses on the design of competency-based performance assessment in e-learning. Though effort has been invested in designing powerful e-learning environments, relatively little attention has been paid to the design of valid and reliable assessments in such environments, leaving many questions to educational developers and teachers. As a solution to this problem, a systematic approach to designing performance assessments in e-learning contexts is presented, partly based on the 4C/ID model. This model enables the construction of realistic whole tasks instead of advocating education that is restricted to more isolated skills. A new assessment procedure also implies an alternative view of instructional design, learning and assessment. The requirements for the learning environment are addressed. Examples from a virtual seminar are presented to illustrate the design approach. The article concludes with the identification of possible pitfalls related to the approach and gives directions for future research.     

Achieving accurate metrics using balanced scorecards and dashboards

The balanced scorecard was developed to align business practices with the overall strategy of an organization and to monitor performance. A dashboard is a computer interface designed to receive and manipulate data from the various departments within a company to present comprehensive information to decision makers. The balanced scorecard, when combined with the dashboard, provides a way for organizations to determine strategy and evaluate performance.     

Sexuality education： Analysis of Moroccan teachers＇ and future teachers＇ conceptions

Conceptions are analyzed as being the emergences from interactions between three poles： scientific knowledge （K）, values （V） and social practices （P）. The teachers＇ beliefs and values have a direct influence on the way of understanding and teaching a topic. These beliefs must be taken into account in the content and strategies of the teacher＇s formation. In this paper, the authors discuss the importance of studying and analyzing these conceptions and present the results of the research on teachers＇ and future teachers＇ conceptions about sexual health education. In this topic, the conceptions could be deeply rooted not only in scientific knowledge but also in value systems and social practices, because teachers often refuse to teach the ethical, psychological, economic and social issues not strictly reducible to biology, such as sexuality health. The authors used a questionnaire prepared jointly within the European project Biohead-Citizen.     

Modes of learning and profitability in Colombian and Mexican SMEs

The aim of this study is to analyze the effects of different modes of learning on innovation and profitability in SMEs in two Latin American countries; Mexico and Colombia. The data were obtained using a sample of 250 companies in the industrial sector operating in both countries. Results show that an integrated mode of learning based on internal and external knowledge supported by technological infrastructure have positive and significant effects on the degree of innovation and the profitability in SMEs However this effect appears to be not significantly different from the traditional SMEs mode of learning based mainly on market orientation.     

Peer Assessment Training in Teacher Education: Effects on performance and perceptions

The implementation of peer assessment receives much attention in teacher education. This paper reports the effects of peer assessment training on the performance of student teachers. Ninety-three student teachers were randomly assigned to control groups and experimental groups. The experimental groups were trained in defining performance criteria, giving feedback and writing assessment reports. This was done through peer assessment tasks that were embedded in a redesigned course. Analyses of data derived from peer assessment reports written by the students showed that the experimental groups surpassed the control groups in the quality of the assessment skill. As a result of the training, students from the experimental groups also scored significantly higher grades for the end products of the course than students from the control groups. The results of the questionnaire showed that all students were significantly more satisfied with the redesigned course.