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1.
Induction of diabetes by Streptozotocin in rats 总被引:1,自引:0,他引:1
A. Akbarzadeh D. Norouzian M. R. Mehrabi Sh. Jamshidi A. Farhangi A. Allah Verdi S. M. A. Mofidian B. Lame Rad 《Indian journal of clinical biochemistry : IJCB》2007,22(2):60-64
The objective of this study is to induce experimental diabetes mellitus by Streptozotocin in normal adult Wistar rats via
comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and C-peptide
in serum, between normal and diabetic rats. Intra-venous injection of 60mg/kg dose of Streptozotocin in adult wistar rats,
makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes mellitus
in the 2–4 days. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic
Langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats.
Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with non-ketosis hyperglycemia in some animal
species. For induction of experimental diabetes in male adult rats weighted 250–300 grams (75–90 days), 60mg/kg of Streptozotocin
was injected intravenously. Three days after degeneration of beta cells, diabetes was induced in all animals. The diabetic
and normal animals were kept in the metabolic cages separately and their body weight, consumption of food and water, urine
volume, the levels of serum glucose, insulin and C-peptide quantities in all animals were measured and then these quantities
were compared. For a microscopic study of degeneration of Langerhans islet beta cells of diabetic rats, sampling from pancreas
tissue of diabetic and normal rats, staining and comparison between them, were done. Induction of diabetes with Streptozotocin
decreases Nicotinamide-adenine dinucleotide (NAD) in pancreas islet beta cells and causes histopathological effects in beta
cells which probably intermediates induction of diabetes. In this study, we used Streptozotocin for our experiments in induction
of experimental diabetes mellitus. After Induction of diabetes, consumption of food and water, volume of urine and glucose
increased in the diabetic animals in comparison with normal animals, but the weight of body and the volume of insulin and
C-peptide decreased in the diabetic animals. Sampling and staining of pancreas tissue of diabetic and normal rats showed that
the Langerhans islet beta cells of diabetic rats have been clearly degenerated. In three days, Streptozotocin makes pancreas
swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes. It also changes normal
metabolism in diabetic rats in comparison with normal rats. Consumption of water and food, volume of urine, serum glucose
increases in diabetic animals in comparison with normal rats but the levels of serum insulin, C-peptide and body weight decreases. 相似文献
2.
Vahid Aryadoust Sanaz Akbarzadeh Sara Akbarzedeh 《Asia Pacific Journal of Education》2011,31(1):51-64
The Multidimensional School Anger Inventory–Revised (MSAI-R) is a measurement tool to evaluate high school students' anger. Its psychometric features have been tested in the USA, Australia, Japan, Guatemala, and Italy. This study investigates the factor structure and psychometric quality of the Persian version of the MSAI-R using data from an administration of the inventory to 585 Iranian high school students. The study adopted the four-factor underlying structure of high school student anger derived through factor analysis in previous validation studies, which consists of: School Hostility, Anger Experience, Positive Coping, and Destructive Expressions. Confirmatory factor analysis of this four-factor model indicated that it fit the data better than a one-factor baseline model, although the fit was not perfect. The Rasch model showed a very high internal consistency among items, with no item misfitting; however, our results suggest that to represent the construct sufficiently some items should be added to Positive Coping and Destructive Expression. This finding is in agreement with Boman, Curtis, Furlong, and Smith's Rasch analysis of the MSAI-R with an Australian sample. Overall, the results from this study support the psychometric features of the Persian MSAI-R. However, results from some test items also point to the dangers inherent in adapting the same test stimuli to widely divergent cultures. 相似文献
3.
Jyoti P. Gurung Moein Navvab Kashani Sanaz Agarwal Gonzalo Peralta Murat Gel Matthew A. B. Baker 《Biomicrofluidics》2021,15(3)
Many motile bacteria are propelled by the rotation of flagellar filaments. This rotation is driven by a membrane protein known as the stator-complex, which drives the rotor of the bacterial flagellar motor. Torque generation is powered in most cases by proton transit through membrane protein complexes known as stators, with the next most common ionic power source being sodium. Sodium-powered stators can be studied through the use of synthetic chimeric stators that combine parts of sodium- and proton-powered stator proteins. The most well studied example is the use of the sodium-powered PomA-PotB chimeric stator unit in the naturally proton-powered Escherichia coli. Here we designed a fluidics system at low cost for rapid prototyping to separate motile and non-motile populations of bacteria while varying the ionic composition of the media and thus the sodium-motive force available to drive this chimeric flagellar motor. We measured separation efficiencies at varying ionic concentrations and confirmed using fluorescence that our device delivered eightfold enrichment of the motile proportion of a mixed population. Furthermore, our results showed that we could select bacteria from reservoirs where sodium was not initially present. Overall, this technique can be used to implement the selection of highly motile fractions from mixed liquid cultures, with applications in directed evolution to investigate the adaptation of motility in bacterial ecosystems. 相似文献
4.
A. Akbarzadeh D. Noruzian Sh. Jamshidi A. Farhangi M. R. Mehrabi B. Lame Rad M. Mofidian A. Allahverdi 《Indian journal of clinical biochemistry : IJCB》2007,22(1):71-76
Insulin injection is the main way to combat against insulin-dependent diabetes mellitus effects. Today in some laboratories
in the world, the investigators are trying to find some treatments for this disease with insulin-secreting pancreatic islet
cells transplantation. Donor tissue in each step of work was prepared from 36 adult male wistar Rats weighted 250–300 grams
(75–90 days). Transplantation was done in rats after 2–4 weeks induction of diabetes with 60mg/kg of streptozotocin injection
by intravenous method. Encapsulation of pancreatic islet cells allows for transplantation in the absence of immunosuppression.
This technique that is called “immunoisolation” is based on the principle that transplanted tissue is protected for the host
immune system by an artificial or natural membrane. In this study, the levels of insulin, C-peptide and glucose in diabetic
rats have been reached to normal range as compared to un-diabetic rats in 20 days after transplantation of islet cells, so
that testis is immunoisolated place for islet cells transplantation. Inside the testis subcutaneously and intrapretoneally
implantation of pure islet cells graft, that is a natural immunoisolation method, rapidly and permanently normalized the diabetic
state of streptozocin-administered animals. 相似文献
5.
Maryam Farahnak Zarabi Ali Farhangi Samaneh Khademi Mazdeh Zahra Ansarian Davod Zare Mohammad Reza Mehrabi Azim Akbarzadeh 《Indian journal of clinical biochemistry : IJCB》2014,29(2):154-160
Carboxylate-modified gold nanoparticles (GNPs) were synthesized in a simple one-step process based on the reduction of tetrachloroauric acid by aspartic acid in water. GNPs were identified by UV–Vis spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy. Conjugation of protein molecules with functionalized nanoparticles was performed through electrostatic interaction. The GNP–protein conjugates were characterized by gel electrophoresis. The interaction between functionalized GNPs and protein molecules lead to conformational transition of protein structure after conjugation of protein with GNPs. This process was investigated by fluorescence spectroscopy and circular dichroism spectroscopy. 相似文献
6.
Ali Farhangi Dariush Norouzian Mohammad Reza Mehrabi Mohsen Chiani Zahra Saffari Maryam Farahnak Azim Akbarzadeh 《Indian journal of clinical biochemistry : IJCB》2014,29(4):406-417
The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult Wistar rats via comparison of changes in body weight, consumption of food, volume of water, urine and levels of glucose, insulin and C-peptide in serum, between normal and diabetic rats. Intra-venous injection of 60 mg/kg dose of streptozotocin in 250–300 g (75–90 days) adult Wistar rats makes pancreas swell and causes degeneration in Langerhans islet β-cells and induces experimental diabetes mellitus in 2–4 days. For a microscopic study of degeneration of Langerhans islet β-cells of diabetic rats, biopsy from pancreas tissue of diabetic and normal rats, staining and comparison between them, were done. In this process, after collagenase digestion of pancreas, islets were isolated, dissociated and identified by dithizone method and then with enzymatic procedure by DNase and trypsin, the islet cells changed into single cells and β-cells were identified by immune fluorescence method and then assayed by flow-cytometer. Donor tissue in each step of work was prepared from 38 adult male Wistar rats weighted 250–300 g (75–90 days). Transplantation was performed in rats after 2–4 weeks of diabetes induction. In this study, the levels of insulin, C-peptide and glucose in diabetic rats reached to normal range as compared to un-diabetic rats in 20 days after transplantation of islet cells. Transplantation was performed under the cortex of testis as immunoisolated place for islet cells transplantation. 相似文献
7.
Azim Akbarzadeh Dariush Norouzian Ali Farhangi Mohammad Reza Mehrabi Shirin Jamshidi Davood Zare Morvarid Shafiei 《Indian journal of clinical biochemistry : IJCB》2008,23(1):57-61
Flow cytometry has been employed as a method to study homogeneity of isolated islet subpopulations. After collagenase digestion
of rat pancreas and elutriation of tissue fragments, islets were isolated and dissociated, and cells were analyzed and sorted
according to their low forward angle light scattering properties by using automated flow cytometry. A standardized procedure
was developed for the preparation of rat islet cell grafts for purification of islet cells. In this process, after collagenase
digestion of pancreas, islets were isolated, dissociated, identification by dithizone method and then with enzymatic procedure
by DNase and trypsin, the islet cells changed into single cells and beta cells were identified by immunofluorescence method
and then assayed by flow cytometry. Methods have been developed for the preparation of suspension of viable rat pancreatic
islet cells and their analysis and sorting in the fluorescence activated cell sorter (FACC IV, Becton Dickinson, Sunnyvale,
Ca). Flow cytometry of these cells indicated that there were 91% of beta cells in cell suspension. Most of the exocrine particles
were lost during digestion. Purified endocrine islet cell grafts were prepared by pure beta-cells, without endocrine non-beta
cells. The purified aggregates were devoid of endocrine non-beta cells and damaged cells. 相似文献
8.
Sanaz Ahmadpoor Samani Siti Zaleha Abdul Rasid Saudah bt Sofian 《Performance Improvement》2015,54(9):28-35
The purpose of this article is to provide a review of background information regarding perceived level of personal control over the physical features of the work environment and its impact on individual satisfaction and outcomes. In today's business with the fast growth of technology, e‐market development, and innovative methods of communication, the workplace will continue to change rapidly. To control and accommodate these fast changes while sustaining or enhancing outcomes, organizations have increasingly turned to a model of work teams; therefore, employees’ skills in working within team environments has been emphasized. To guarantee that the work environment itself supports these new approaches of working, flexible workplaces are often suggested. Open‐plan offices offer more flexibility when compared to completely closed and private ones and they are considered to have more capabilities and are, therefore, highly valued in today's industry. Apart from its advantages, open‐plan office design does create some problems due to lack of personal control over the work environment, which can negatively affect employees’ satisfaction and performance. It is, therefore, important to emphasize the significance of personal control over the physical work environment and the impact of that on employees’ satisfaction and outcome. 相似文献
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10.
Sanaz Farhangi 《Cultural Studies of Science Education》2012,7(4):1037-1044
This paper presents a review of Jane McGonigal’s book, “Reality is broken” (Reality is broken: why games make us better and how they can change the world. Penguin Press, New York, 2011). As the book subtitle suggests it is a book about “why games make us better and how they can change the world”, written by a specialist in computer game design. I will try to show the relevance this book might have to science educators through emphasizing the points that the author offers as the fixes to rebuild reality on the image of gaming world. Using cultural-historical activity theory, I will explore how taking up a gamer mindset can challenge one to consider shortcomings in current approaches to the activity of teaching–learning science and how using this mindset can open our minds to think of new ways of engaging in the activity of doing science. I hope this review will encourage educators to explore the worldview presented in the book and use it to transform our thinking about science education. 相似文献