人类肥胖基因克隆和表达

以编码人全长的OBmRNA为模板,用RT-PCR法获得了编码146个氨基酸残基的OBcDNA,并经EcoRI和BamHI双酶切克隆至原核质表达质粒pGEX-1λT中,通过IPTG诱导,以谷胱苷肽巯基转移酶融合蛋白(GSP)方式进行了表达。结论:在原核细胞中获得高效表达。     

运动对Leptin的影响及Leptin调节代谢的某些相关因素

消脂素 (L eptin,L P)在体重调节方面的作用是肯定的 ,而不同运动对其影响不同。随着分子生物学技术的发展 ,有关 L P的生物学特性已获得大量资料 ,且证实血 L P调节代谢同多种因素相关 ,运动作为刺激手段通过不同途径影响 L P。     

长期有氧运动一次性运动和瘦素

本文综述了当前运动对Lepfin效应的主要研究工作。Lepfin是近年发现的能量平衡调节的激素，是Ob基因的表达产物。运动对Leptin水平的影响表现在：长期有氧运动可降低血Lepfin浓度，减少脂肪组织Ob基因的表达，而一次性运动对Lepdn表达和其血中浓度的影响研究结果不一。运动的这些效应可能依赖于运动负荷量和运动时间。     

组合型人工肾对慢性肾衰患者血清瘦素、同型半胱氨酸及甲状旁腺素水平的影响

目的：探讨血液灌流加血液透析对慢性肾衰患者血清中瘦素（Leptin）、同型半胱氨酸（tHcy）及甲状旁腺素（iPTH）的清除作用,并与单纯血液透析清除Leptint、Hcy、iPTH的效果比较。方法：在25例慢性肾衰患者常规血液透析中增加一次血液透析加灌流治疗,在透析及透析加灌流前后分别检测其Leptint、Hcy、iPTH血清水平,结果表明血透加灌流治疗后较治疗前明显降低（P〈0.01）,且较单纯血液透析明显降低（P〈0.05）,而单纯血液透析前后血清Leptint、Hcyi、PTH水平没有改变（P〉0.05）。结论：血液灌流可以明显降低慢性肾衰患者血清中的Leptin、tHcyi、PTH水平,从而改善透析患者的生活质量,减少营养不良、心血管疾病和肾性骨病和异位钙化并发症的发生。     

瘦素及其临床意义

瘦素是一种蛋白激素,对调节体重、新陈代谢、生育功能有重要影响,具有广泛的生物学意义.在临床上瘦素浓度还被发现和肥胖症、心血管疾病、Ⅱ型糖尿病、不育症、骨质疏松、肾脏病、骨关节炎、脂肪肝和乳腺癌等许多疾病有关联.     

瘦素、肥胖与运动

本文主要阐述肥胖基因表达产物——瘦素（Leptin,LP）的结构、功能及其作用,着重探讨瘦素与肥胖和运动的关系,并对其研究前景提出展望．     

营养不足与运动性月经周期紊乱及Leptin调节

大量报道发现运动训练可引发女运动员出现月经周期紊乱 ,其发生主要涉及体成份和训练安排等多方面因素。通过对运动员的营养调查发现 ,营养不足作为其中一个因素 ,在运动性经周期紊乱的形成中具有重要作用。而且ob基因产物Leptin ,作为内部调节物质 ,可随体内营养状况不同 ,而通过调节HPO轴来改变月经周期。     

Leptin的生物学特征及其在控体重方面机制的研究

Leptin具有广泛的生物学功能,可以在人体机能调节过程中发挥重要作用。通过对相关的文献的整理、归纳,对Leptin的生物学特点及其在控体重机制方面研究进展和问题进行了总结和讨论,力求为未来与Leptin相关的体育学研究提供参考和依据。     

Study of Association of Leptin and Insulin Resistance Markers in Patients of PCOS

Polycystic ovary syndrome (PCOS) is a common endocrinological disorder among women of the reproductive age group with long term sequelae which include diabetes mellitus, hypertension and CAD. The present study was conducted to evaluate the association of leptin—an adipokine playing an important role in carbohydrate metabolism and markers of insulin resistance among women with PCOS. Sixty diagnosed cases of PCOS as per Rotterdam criteria were enrolled in this study after informed written consent. Insulin resistance was estimated using the homeostatic model assessment-insulin resistance (HOMA-IR). HOMA-IR was calculated as the product of the fasting plasma insulin value (mU/ml) and the fasting plasma glucose value (mg/dl), divided by 405 and HOMA β was calculated as 360 × [insulin]/([glucose] − 63) % (glucose in mg/dl). Estimation of serum leptin levels was done by ELISA using leptin ELISA kit from (DRG). A positive correlation of serum leptin levels was observed with markers of insulin resistance. Multiple regression analysis with HOMA-IR as dependent variable demonstrated a statistically significant contribution of fasting insulin levels. This study highlights the role of leptin in alterations in carbohydrate metabolism in patients with PCOS.     

Assessment of ghrelin and leptin receptor levels in postmenopausal women who received oral or transdermal menopausal hormonal therapy

Objective  

In postmenopausal women, an increased leptin concentration and reduced levels of ghrelin and adiponectin were observed. The aim of this study was to evaluate the concentrations of the active form of ghrelin, total ghrelin, leptin receptor, lipoprotein(a) (Lp(a)), and plasminogen activator inhibitor type 1 (PAI-1) in postmenopausal women who received oral or transdermal menopausal hormonal therapy (MHT).