奇圣胶囊对运动员某些生化指标影响的实验观察

通过为期4周的实验，从运动生化角度观察了奇圣胶囊对广东省现代五项队运动员身体机能状态和运动能力的影响，结果表明，补充奇圣胶囊2周后能显著提高血红蛋白值，降低定量负荷运动后的血乳酸值，说明奇圣胶囊对于有乳能力提高有促进作用；补充奇圣胶囊能保持血睾酮水平的基本稳定，皮质醇明显下降，两者比值提高，有利于运动后的恢复，有助于身体对运动的适应和机能的提高；补充奇圣胶囊2周后能显著提高糖酵解能商(LQ)水平，对糖酵解供能系统供能能力有显著作用。     

针刺足三里穴对运动小鼠红细胞形态、血红蛋白浓度的影响

运用扫描电镜和全自动血球分析仪研究针刺足三里穴对运动小鼠红细胞形态和血红蛋白浓度的影响 ,结果发现运动组小鼠红细胞畸形率显著高于正常组、针刺组和假针刺组 (均P <0 0 1) ,针刺组小鼠红细胞畸形率分别高于正常组和假针刺组 (均P <0 0 1) ,正常组与假针刺组小鼠红细胞畸形率差异无显著性 (P >0 0 5 ) ,运动组小鼠HGB浓度显著低于正常组、针刺组和假针刺组 (均P <0 0 1) ,针刺组、正常组和假针刺组小鼠红细胞HGB浓度差异无显著性 (P >0 0 5 )。表明针刺足三里穴能明显降低大强度训练小鼠红细胞畸形率 ,提高HGB浓度 ,为针刺足三里穴在体育实践中的运用提供了实验依据。     

国家皮划艇运动员高原训练的机能监测和评价研究

为了探索皮划艇运动员的高原训练，对国家皮划艇集训队1999年的两次高原训练，进行了全程跟踪监测，以期对皮划艇运动员的高原训练进行评价。监测结果表明：(1)皮划艇运动员上高原后7至10d可完全适应高原环境训练，高原训练要考虑适应的时间和转换运动训练负荷强度的时机。(2)高原训练的负荷应安排在高原训练期间出现两次血红蛋白的高峰。血红蛋白的动态变化对于监控和保证高原训练负荷的量和强度是一个有益的指标。下山后14d和40d血红蛋白有两个明显的高峰，揭示可能有两次运动能力的高潮点。(3)高原训练后完成500m和1000m血乳酸的浓度显性增加，揭示运动员耐酸能力提高，500m和1000m成绩有显性提高。     

微柱法测定糖化血红蛋白方法的改进

目的 :建立一种在临床上比较实用的GHb的测定方法。方法 :将Bio -Rex70阳离子交换树脂溶液与溶血液充分振荡、混匀后 ,离心 ,测定上清液的吸光度值。结果 :变异系数 :,正常标本CV % =3、3% ;高值标本CV % =4、5 %。方法比较 ,与泰康公司微柱法试剂盒比较r=0 .94 ,密切相关。灵敏度 ,0 .0 1A相当于GHb为 0 .3%。结论 :经改进的微柱法 ,简便、快速 ,性能符合临床要求     

血红蛋白指标在少年跆拳道运动员训练中的运用

通过在少年跆拳道训练中运用血红蛋白指标对运动员身体机能的监控，探讨了血红蛋白指标对少年跆拳道训练的重要指导作用。     

高原训练对人体呼吸和循环系统的影响

高原训练的生理机制通常被认为是机体对氧不足引起的血氧过少的适应结果。机体对高原的低氧环境很快会建立起一系列复杂的代偿性生理机制,使各系统达到新的平衡,即所谓习服。而这种习服首先对人体呼吸、循环系统产生影响。     

运动训练对优秀女子中长跑运动员铁代谢的影响

目的:探究女子中长跑运动员铁代谢的变化规律;方法:动态测定2个月训练内蒙古女子中长跑运动员的血红蛋白、血清铁、铁蛋白、转铁蛋白、转铁蛋白受体含量、红细胞分布宽度和促红细胞生成素水平;结果:优秀女子中长跑运动员训练期血红蛋白含量先下降后上升;血清铁含量具有先下降后上升趋势,但不显著;铁蛋白含量未见明显改变;转铁蛋白含量先升后降;转铁蛋白受体含量在训练中变化不明显;血红细胞分布宽度先升高后下降,EPO水平先升后降;结论:在训练初期,由于运动训练,机体铁代谢出现紊乱,而后通过调整恢复正常.     

Interaction of hemoglobin Grey Lynn (Vientiane) with a non-deletional α+-thalassemia in an adult Thai proband

Hemoglobin (Hb) Grey Lynn is a Hb variant caused by a substitution of Phe for Leu at position 91 of α1-globin chain, originally described in individual of unknown ethnic background. This article addresses the interaction of Hb Grey Lynn with a non-deletional α⁺-thalassemia found in Thailand, a hitherto un-described condition. The proband was adult Thai woman referred for investigation of mild anemia with Hb 90 g/L. Hb analyses using low pressure liquid chromatography raised a suspicion of abnormal Hb presence, which was failed to demonstrate by cellulose acetate electrophoresis and capillary electrophoresis. DNA sequencing identified a CTT (Leu) to TTT (Phe) mutation at codon 91 corresponding to the Hb Grey Lynn (Vientiane) [α91(FG3)Leu>Phe (α1) on α1-globin gene and a C deletion between codons 36 and 37 on α2-globin gene causing α⁺-thalassemia. As compared to those observed in a compound heterozygote for Hb Grey Lynn / α⁰-thalassemia reported previously, higher MCV (81.7 fL) and MCH (26.3 pg) values with a lower level of Hb Grey Lynn (19.7%) were observed in the proband. The normochromic normocytic anemia observed could be due to the interaction of Hb Grey Lynn with α⁺-thalassemia. The two mutations could be identified using PCR-RFLP and allele-specific PCR assays developed.     

70-year old female patient with mismatch between hematocrit and hemoglobin values: the effects of cold agglutinin on complete blood count

Introduction:

There are a number of pre-analytical and analytical factors, which cause false results in the complete blood count. The present case identifies cold agglutinins as the cause for the mismatch between hematocrit and hemoglobin values.

Materials and methods:

70-year old female patient had a history of cerebrovascular diseases and rheumatoid arthritis. During routine laboratory examination, the patient had normal leukocyte and platelet counts; however, the hemoglobin (Hb: 105 g/L) and hematocrit (HCT: 0.214 L/L) results were discordant. Hemolysis, lipemia and cold agglutinin were evaluated as possible reasons for the mismatch between hematocrit and hemoglobin values.

Results:

First blood sample was slightly hemolysed. Redrawn sample without hemolysis or lipemia was analyzed but the mismatch became even more distinct (Hb: 104 g/L and HCT: 0.08 L/L). In this sample, the titration of the cold agglutinin was determined and found to be positive at 1:64 dilution ratios. After an incubation of the sample at 37°C for 2 hours, reversibility of agglutination was observed.

Conclusion:

We conclude that cold agglutinins may interfere with the analysis of erythrocyte and erythrocyte-related parameters (HCT, MCV, MCH and MCHC); however, Hb, leukocyte and platelet counts are not affected.     

Diagnostic Dilemma of HbA1c Detection in Presence of a Hemoglobinopathy: A Case Report

We report a case of a diabetic, heterozygote with near normal hematology, marginally low level of hemoglobin A₂(HbA₂) having an increased level of hemoglobin F(HbF) that was pancellularly distributed among the red cells. BioRad DiaSTAT measurements gave a high glycated hemoglobin A1c(HbA1c) of 31.5% and the BioRad Variant analyzer recorded an HbA1c value which was very low, in discordance with the detected blood glucose levels. Flow cytometry and polymerization chain reaction (PCR) based studies were carried out which revealed the case to be that of the common hereditary persistence of fetal hemoglobin (HPFH)-3, an Asian Indian mutation. Fructosamine estimation and HbA1c by Boronate affinity chromatography were able to resolve the discordant value detected and was able to confirm the diabetes status. The case would have been a diagnostic dilemma, if reported without correlation.