| 筋骨草中槲皮素成分的含量测定 |
| |
| 引用本文： | 陈建琴,许雪琴.筋骨草中槲皮素成分的含量测定[J].莆田学院学报,2008,15(5). |
| |
| 作者姓名： | 陈建琴 许雪琴 |
| |
| 作者单位： | 1. 莆田学院,环境与生命科学系,福建,莆田,351100
2. 福州大学,化学系,福建,福州,350002 |
| |
| 摘 要： | 采用高效液相色谱法对筋骨草中槲皮素的含量进行测定。色谱条件为:色谱柱,Hypersil ODS(5μm,250.0×4.0mm);流动相,pH=2.08磷酸:甲醇为45:55;检测波长为370nm。标准曲线显示,槲皮素在2.24—22.40μg·mL-1范围内呈良好的线性关系,回归方程为y=192.0x+202.4,r=0.998。样品平均回收率为97.6%,RSD为1.67%,这表明本法是一种筋骨草中槲皮素的分析及含量测定的简便、快速、准确且重现性好的方法。
|
| 关 键 词： | 筋骨草 槲皮素 含量 测定 高效液相色谱法 |
Determination of Quercetin in the Ajuga Decumbent by HPLC |
| |
| CHEN Jian-qin,XU Xue-qin.Determination of Quercetin in the Ajuga Decumbent by HPLC[J].journal of putian university,2008,15(5). |
| |
| Authors: | CHEN Jian-qin XU Xue-qin |
| |
| Institution: | CHEN Jian-qin1,XU Xue-qin2 ( 1. Environment & Life Sciences Department,Putian University,Putian Fujian 351100,China,2. Department of Chemistry,Fuzhou University,Fuzhou Fujian 350002,China ) |
| |
| Abstract: | Objective: To analysis and determine the content quercetin in Ajuga Decumbent. The Hypersil ODS (5 μm, 250.0×4.0 mm) column was used. The mobilephase consisted of methano — Phosphoric acid solutions(55:45) with detection wavelength of 370 nm. Results: Linearity of quercetin was in the range of 2.24—22.40 μg·mL-1. The rgerssion equation was y = 192.0x +202.4 with r = 0.998. The average recovery rate was 97.6 % and RSD was 1.67 %. Conclusion: The method is simple, quick, sensitive, accurate, reproducible and can be used to determine the content quercetin in Ajuga Decumbent. |
| |
| Keywords: | Ajuga Decumbent quercetin content determination HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
