鹅MYL1基因的克隆及胚胎期表达特征分析

肌球蛋白是肌纤维的主要组成成分，在肌肉生长和收缩过程中具有重要作用.本研究以鹅（Anser anser）肌肉总RNA为模板，采用RACE的方法，克隆肌球蛋白轻链1（MYL1）基因全长cDNA序列，并进行生物信息学分析.运用实时荧光定量PCR检测MYL1基因在鹅胚胎期的表达特征.结果表明，鹅MYL1基因全长cDNA为1542 bp，编码193个氨基酸残基的肽链.预测鹅MYL1蛋白等电点5.12，分子量21.75 KD，具有典型的EFh和FRQ1结构域，且不同物种间EFh氨基酸序列高度同源.荧光定量PCR结果显示鹅胚胎期MYL1基因mRNA表达量总体呈现上升后下降的趋势，该基因在胚胎期E7就有表达，E7以后表达量逐渐上升，在E18表达量达到高峰后下降.研究结果首次提供了鹅肌肉组织主要结构基因MYL1的全序列和蛋白特征信息，并揭示该基因在鹅肌肉组织发生和发育的功能.

Molecular Cloning of Goose MYL1 Gene and Its Expression during Embryonic Development

Myosin is a major component of muscle fibers in animal, and plays an important role in the growth and contraction of muscle. RT-PCR and rapid-amplification cDNA ends （RACE） were used to obtain a full-length of MYL1 cDNA. A full-length of 1 542 bp MYL1 cDNA included a 582- bp open reading frame en-coding a polypeptide of 193 amino acids, with a calculated relative molecular mass of 21.75 KD and pI of 5.12. The putative protein encoded by goose MYL1 gene had the conservative domains EFh and FRQ1. Real-time PCR was performed to analyze the expression of MYL1 gene during embryonic period, and the results showed that MYL1 gene was expressed at an early stage of embryogenesis, after that gradually increased, reached the peak at E18, and then decreased. The current study first provided the complete sequence information and molec-ular structural of goose MYL1 gene, and revealed its function in goose myogenesis and development.