| 基于纳米金／甲苯胺蓝／多壁碳纳米管复合物膜修饰的高灵敏的电流型癌胚抗原免疫传感器的研究 |
| |
| 引用本文： | 李娜,甘贤雪,王丽.基于纳米金／甲苯胺蓝／多壁碳纳米管复合物膜修饰的高灵敏的电流型癌胚抗原免疫传感器的研究[J].重庆职业技术学院学报,2009,18(3):80-82. |
| |
| 作者姓名： | 李娜 甘贤雪 王丽 |
| |
| 作者单位： | 宜宾学院,化学化工系,四川,宜宾,644007 |
| |
| 摘 要： | 以固载于玻碳电极（GC）表面的壳聚糖／多壁碳纳米管（MWNT～CS）复合物膜为基底电聚合媒介体甲苯胺蓝（PTOB），利用静电作用吸附纳米金（nano--Au），再利用纳米金良好的生物兼容性、大的比表面积固定癌胚抗体（anti—AFP），从而制得高灵敏、高稳定电流型癌胚抗原免疫传感器。多壁碳纳米管复合物膜促进了电子的传递．增大了电极的比表面积：纳米金的存在增加了抗体在电极表面的固定量，从而提高了免疫电极的灵敏度。在优化的实验条件下．该免疫电极在1．0～80．0ng／mL范围内，峰电流与CEA抗原的浓度呈良好的线性关系。检测线为0．4ng／mL。该免疫传感器制备方法简单，灵敏度高、选择性好。
|
| 关 键 词： | 碳纳米管 纳米金 癌胚抗原 免疫传感器 |
A Highly Sensitive Amperometric Immunosensor for the Determination of Carcinoembryonic Antigen Based on Gold Nanoparticles/Toluidine Blue O/MWNT Modified Composite Membrane |
| |
| LI Na,Gan Xianxue,WANG Li.A Highly Sensitive Amperometric Immunosensor for the Determination of Carcinoembryonic Antigen Based on Gold Nanoparticles/Toluidine Blue O/MWNT Modified Composite Membrane[J].Journal of Chongqing Vocational& Technical Institute,2009,18(3):80-82. |
| |
| Authors: | LI Na Gan Xianxue WANG Li |
| |
| Institution: | LI Na,Gan Xianxue,WANG Li(Department of Chemistry , Chemical Engineering,YiBin University,YiBin Sichuan 644007,China) |
| |
| Abstract: | A new amperometric immunosensor for the determination of carcinoembryonic antigen(CEA) has been developed by means of immobilization carcinoembryonic antibody onto the nano-Au/poly(toluidine blue O)/MWNT-CS modified composite film.Under optimal conditions,the immunosensor displayed a high-sensitivity for the detection of CEA and had good correlation for the detection of CEA in the range of 1.0 to 80.0 ng/ml with a detection limit of 0.4 ng/ml.The proposed methodology was economical,efficient and potentially... |
| |
| Keywords: | Carbon nanotubes Gold nanoparticles carcinoembryonic antigen immunosensor |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
