| Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu^2＋ |
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| 引用本文： | Yu HN,Shen SR,Xiong YK. Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu^2＋[J]. Journal of Zhejiang University. Science. B, 2005, 6(2): 125-131 |
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| 作者姓名： | Yu HN Shen SR Xiong YK |
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| 作者单位： | Department of Tea Science,Department of Tea Science,Department of Pharmacology School of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310009,China,School of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310009,China,Zhejiang College of Traditional Chinese Medicine,Hangzhou 310009,China |
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| 摘 要： | Epigallocatechin-3-gallate (EGCG) has shown remarkably anti-cancer activity, with its bioactivity being related to reactive conditions, such as pH and metal ions. The present study investigated the degradation of EGCG and its effect on prostate cancer cell in the presence of Cu2 . EGCG was incubated with prostate cancer cells,
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| 关 键 词： | 铜离子 LNCaP细胞 绿茶 EGCG DNA 流量 |
Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu2+ |
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| Yu Hai-ning,Shen Sheng-rong,Xiong Yao-kang. Cytotoxicity of epigallocatechin-3-gallate to LNCaP cells in the presence of Cu2+[J]. Journal of Zhejiang University. Science. B, 2005, 6(2): 125-131 |
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| Authors: | Yu Hai-ning Shen Sheng-rong Xiong Yao-kang |
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| Affiliation: | Department of Tea Science, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310009, China. |
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| Abstract: | Epigallocatechin-3-gallate (EGCG) has shown remarkably anti-cancer activity, with its bioactivity being related to reactive conditions, such as pH and metal ions. The present study investigated the degradation of EGCG and its effect on prostate cancer cell in the presence of Cu2 . EGCG was incubated with prostate cancer cells, LNCaP, pretreated with or without Cu2 .EGCG in F-12 medium was quantified using HPLC and the viability of cells was assessed by gel electrophoresis, flow cytometry,and electron microscope. The results of HPLC showed that EGCG degraded completely within 12 h in F-12 medium with or without Cu2 . Gel electrophoresis and flow cytometry did not detect apoptosis of LNCaP cells when they were incubated with EGCG. Electron microscopy examination revealed that EGCG-Cu2 complex led to damage of cytoplasm membrane in LNCaP cells. It was speculated that not EGCG, but its oxide and complex with Cu2 , are the bioactive components responsible for its cytotoxicity to LNCaP prostate cancer cells. |
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| Keywords: | Copper Epigallocatechin-3-gallate LNCaP cells Tea |
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