运动疲劳对大鼠黑质网状部小清蛋白表达的影响

目的:观察运动疲劳及恢复阶段大鼠黑质网状部(substantia nigra pars reticulata,SNr)小清蛋白(Parvalbumin,PV)表达水平的变化,探讨其在运动疲劳中枢调控中的作用。方法:雄性SD大鼠随机分为对照组和运动组,采用免疫组织化学方法观察7天反复力竭跑台运动后即刻、24h和48h SNr内PV的表达变化情况。结果:PV阳性细胞和纤维集中分布于SNr外侧约2/3的区域,SNr内PV表达水平在短期内显著下降(P0.01),恢复24h后明显回升(P0.01),并随着恢复时间的延长逐渐达到对照组水平(P0.05)。结论:运动疲劳可导致SNr内PV表达水平短时间内显著性的下降,同时PV阳性表达集中分布于SNr的外侧区,此区域与SNr接收纹状体腹外侧区神经投射区域相一致,是参与基地神经节运动调控的重要脑区。

Effects of Exercise Induced Fatigue on the Expression of the Substantia Nigra Pars Reticulata Parvalbumin in Rats

Objective：To observe the sports fatigue and recovery phase in the rat substantia nigra mesh division （ the substantia nigra pars reticulata , SNr） Parvalbumin （ PV） the change of expression level , discusses its role in the sports fatigue central control .Methods：male SD rats were randomly divided into control group and exercise group, using immunohistochemical method to observe 7 days repeatedly run immediately after exercise , exhaustion of PV within 24 h and 48 h SNr expression changes .Positive results：PV cells and fiber concentration distribution in SNr lateral, about two-thirds of the area within the SNr PV expression level decreased significantly in a short time （P ＆lt;0.01）, and recovery after 24 h rebounded significantly （P ＆lt;0.01）, and gradually with the extension of recovery time up to the level of the control group （P ＆gt;0.05）.Conclusion：exercise fatigue can lead to SNr in PV expression level in a short period of time significant decline , at the same time PV positive expression on the SNr of the lateral distribution area , the area with SNr ventrolateral area receives the striatum neural projection area is consistent , is one of the important brain areas involved in the basal ganglia movement control .