胰岛素抵抗动物模型和运动干预模型的建立与评价

目的:建立IR动物模型和运动干预模型,为后续实验奠定基础.方法:46只雄性SD大鼠分4组:普食对照组、普食运动组、高脂膳食对照组、高脂膳食运动组.以高脂膳食诱导IR,同时运动组进行10周有氧游泳运动干预.综合利用体重、FINS、FBG、HOMA-IR、ISI、ITT指标评价动物模型的建立和运动干预的效果.结果:(1)E组和C组、H组和C组、HE组和H组间,体重、FINS和ISI有显著性著异(P<0.01或P<0.05);(2)ITT中,0时血糖无差异,在15min、30min、60min时间点,H组明显高于C组,HE组明显低于H组(P<0.05).E组与C组比,15min、30min时明显下降(P<0.05).结论:高脂膳食饲养10周可诱导正常SD大鼠出现IR;运动提高了大鼠胰岛素敏感性:间接评价IR指标中ISI较HOMA-IR敏感.

Building and evalu ation of insulin resistant rats and exercise intervention models

Insulin resistant rat s and exercise intervention model s were built in order to set foundation
for subsequent work. 46 rat s were divided into normal dietary cont rol group , normal exercise group ,
high fat dietary cont rol group and high fat dietary exercise group . High fat dietary were fed and exer2
cise group were given aerobic swimming intervention for ten weeks. Body weight , FINS , FBG, HO2
MA2IR , ISI and insulin tolerant test were used to evaluate building of animal model and effect of exer2
cise intervention. It was shown t hat between E and C , H and C , HE and H group s , there were signif2
icant differences on body weight , FINS and ISI ( p < 0. 01 or p < 0. 05) . In IET , blood of H group
was significant ly higher than t hat of C and that of HE was lower t han t hat of C ( p < 0. 05) at 15min ,
30min and 60min. Compared wit h E , C was lower at 15min and 30min. It could be seen t hat ten
weeks of high fat dietary feeding induced IR in SD rat s. Exercise enhanced rat s insulin sensitivity and
indirect evaluation of IR , ISI was more sensitive t han HOMA2IR.