双歧杆菌介导的福氏志贺菌Bb-ipaB疫苗的构建

目的：构建福氏志贺菌重组双歧杆菌Bb-ipaB疫苗。方法：以福氏志贺菌DNA为模板扩增福氏志贺菌ipaB基因,双酶切后克隆到pGEX-1λT质粒上,构建重组质粒pGEX-ipaB,电穿孔法将该质粒转化入Bb,构建福氏志贺菌重组Bb-ipaB疫苗。结果：成功扩增出分子量约为1 711 bp的ipaB基因,双酶切证实基因成功插入pGEX-1λT中,并成功转化入双歧杆菌,构建rBb-ipaB疫苗。结论：成功构建福氏志贺菌重组rBb-ipaB疫苗,为该疫苗的进一步研究奠定了基础。

Construction of the Recombinant Bb-ipaB Vaccine of Shigella flexneri

Objective：To construct the recombinant Bb-ipaB vaccine of Shigella flexneri. Methods：ipaB gene was amplified by PCR and cloned into pGEX-1 λ T plasmid to construct pGEX-ipaB. The recombinant plasmid was electroporated into Bifidobacteria bifidum （Bb） to construct rBb-ipaB vaccine. Results： A 1 711 bp gene of ipaB was successfully amplified by PCR and cloned into pGEX-1 λ. T by restriction analysis, and the rBb-ipaB vaccine was successfully constructed by PCR and restriction analysis. Conclusion：The rBb- ipaB vaccine of Shigellaflexneri is successfully constructed, which lays the experimental foundation of exploitation and utilization of this vaccine.