不同运动强度对衰老小鼠血清干预效果的研究

目的：研究不同运动强度对D-半乳糖致衰老小鼠的抗氧化水平的影响，观察其延缓表老的效果。方法：将50只昆明小鼠，随机分为5组，分别为大运动强度．中等运动强度、小运动强度的运动干预组，衰老时照组和正常对照组。采用D-半乳糖皮下注射建立亚急性衰老模型。6周后测定血清中与抗衰老作用相关的超氧化物吱化酶（SOD）、谷胱甘肤过氧化物酶（GSH--PX）的活性及丙二醛（MDA）的含量。结果：运动干预组与衰老模型姐比较，大运动强度干预组小鼠血清SOD的活性有显著性改善（P〈0．05），GSH-PX的活性和MDA的含量则无显著性差异；中等运动强度干预组血清SOD，GSH-PX活性均有显著性改（P〈0．05），并且MDA的含量也有显著性改善（P〈0．05）；小运动强度干预组小鼠的血清SOD、GSH-PX的活性和MDA的含量则均无显著性差异。进一步分析表明，与衰老模型组比较，中等运动强度干预组血清SOD、GSH-PX活性和MDA@量的变化幅度均大于其它两种运动强度。结论：研究结果表明中等运动强度的干预能显著改善血清中与抗表老作用相关的血清SOD、GSH—PX的活性和MDA的含量。

Study on the effect of aging mice serum intervention in different exercise intensity

Objective：To investigate the effects of different exercise intensity on anti--oxidation in D--galactose induced aging mice. Methods：D--galactose subcutaneous injection was used to establish sub acute aging model. Fifty Kunming mice were randomly allocated to one of the following five groups：high intensity,moderate intensity,low intensity,ageing comparison and non--ageing control group. After 6 weeks of intervention,serum super oxide dismutase（SOD）,glutathione peroxides（GSH-PX）activity and malondialdehyde（MDA） content were assessed.Results：Compared to non--ageing control group,there were significant deterioration in SOD,GSH--PX and MDA in the aging comparison group（P〈0.05）.Compared to the aging comparison group,there were statistically significant improvements in SOD （P〈0.05）,GSH--PX（P〈0.05） and MDA（P〈0.05）in moderate intensity group and an improvement in SOD（P〈0.05）（but not in GSH and MDA） in high intensity group and no any changes in the low intensity group. Conclusion：The findings of the study showed the effects of moderate exercise intervention on serum SOD,GSH-PX and MDA in D--galactose induced aging mice.