自发性高血压大鼠膈肌功能的变化

目的：研究自发性高血压大鼠（SHR）膈肌功能的变化。方法：大鼠随机分成两组，SHR组和Wistar大鼠组，各8只，应用体外灌流大鼠膈肌条的方法，测量张力一频率曲线，疲劳指数的变化，同时测定膈肌组织中的超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮合酶（NOS）活性（总NOS和iN0s）和NO含量的变化。结果：与对照组相比，SHR组大鼠膈肌张力-频率曲线右移，疲劳指数下降明显（P〈0．01），组织中SOD、总NOS活性和NO含量显著降低（P〈0．01），iNOS、MDA含量显著增加（P＜0．01）。结论：自发性高血压大鼠膈肌功能受到损伤。

Changes of Diaphragmatic Functions in Spontaneously Hypertensive Rats

Objective: To study the change of diaphragmatic functions in Spontaneously Hypertensive Rats(SHR).Methods: Rats were divided into two groups.One group(n=8)was Wistar rats as control and the other(n=8)was SHR group.The force-frequency curve and fatigue index(FI)in vitro diaphragm strip were measured,the activities of superoxide dismutase(SOD)and nitric oxide synthase(NOS)in diaphragm tissues were measured as well.The contents of nitric oxide(NO)and malondiadehyde(MDA)were also detected.Results: Compared with that of the control,the force-frequency curve shifted to right and FI significantly decreased(P< 0.01)in SHR group.Compared with the results of the control,SOD,pant-NOS activities,NO contents were significantly lower(P<0.01),iNOS and MDA contents were significantly higher in SHR group(P<0.01).Conclusion: The diaphragmatic functions were damaged in SHR,iNOS and MDA contents were significantly higher.