| 筋骨草中黄酮类化合物提取工艺的探究 |
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| 引用本文： | 陈建琴,薛美香,黄建辉.筋骨草中黄酮类化合物提取工艺的探究[J].莆田学院学报,2014(2):15-18. |
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| 作者姓名： | 陈建琴 薛美香 黄建辉 |
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| 作者单位： | 莆田学院环境与生物工程学院,福建莆田351100 |
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| 基金项目： | 国家自然科学基金项目（21103095）;福建省教育厅科技项目（JA12285）;福建省中青年教师教育科研项目（JA13279） |
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| 摘 要： | 以乙醇回流提取法提取筋骨草中黄酮类化合物,采用比色法测定总黄酮含量,跟踪乙醇浓度、料液比、提取时间、提取温度等因素对黄酮类化合物提取量的影响,通过正交实验确定最佳的提取工艺条件。并以槲皮素为对照品,通过高效液相色谱法(HPLC)表征了溶液的提取物为黄酮类化合物。结果表明,适宜的提取工艺条件为:乙醇浓度60%,料液比1:20,提取温度为70℃,提取时间为2.5h。
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| 关 键 词： | 筋骨草 黄酮类化合物 比色法 提取工艺 |
The Study of Extracted Technology of Flavonoid from the Ajuga Ciliate |
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| CHEN Jian-qin,XUE Mei-xiang,HUANG Jian-hui.The Study of Extracted Technology of Flavonoid from the Ajuga Ciliate[J].journal of putian university,2014(2):15-18. |
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| Authors: | CHEN Jian-qin XUE Mei-xiang HUANG Jian-hui |
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| Institution: | ( College of Environmental and Biological Engineering, Putian University, Putian Fujian 351100, China ) |
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| Abstract: | The flavonoid was extracted from the Ajuga Ciliate via ethanol reflux extraction method. The extraction content of flavonoid were followed and determined by colorimetry. The effects of the ethanol concentration, ratio of solid to liquid, temperature of extraction and time of extraction on the extraction content were followed. The optimal experimental conditions of extracting the flavonoid were determined through orthogonal experiment. It was proven that the extractive is flavonoid by high performance liquid chromatography（HPLC） with quercetin as reference substance. The results showed that the appropriate extraction process conditions were as follows： ethanol concentration is 60 %; ratio of solid to liquid is 1 ： 20; temperature of extractio n is 70℃; time of extraction is 2.5 hour. |
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| Keywords: | Ajuga Ciliate flavonoid colorimetry reflux extraction |
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