| 辣根过氧化物酶催化H2O2氧化橙黄Ⅱ的降解研究 |
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| 引用本文： | 王亚丽,马明明.辣根过氧化物酶催化H2O2氧化橙黄Ⅱ的降解研究[J].宁夏师范学院学报,2013(6):55-58,73. |
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| 作者姓名： | 王亚丽 马明明 |
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| 作者单位： | 宁夏师范学院化学与化学工程学院,宁夏固原756000 |
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| 基金项目： | 宁夏回族自治区自然科学基金资助项目（NZ13210）;宁夏师范学院科研基金资助项目（YB201327）. |
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| 摘 要： | 研究了pH值、温度、反应时间、过氧化氢用量、染料初始浓度等对辣根过氧化物酶（HRP）催化过氧化氢氧化降解橙黄Ⅱ染料溶液的影响.结果表明在pH值为2、温度为43℃、反应时间为12 min、H2 O2用量为2.0 mmol·L-1、染料初始浓度0.8 mmol·L-1时橙黄Ⅱ的降解最为理想,脱色率可达到83.7％.
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| 关 键 词： | 偶氮染料 橙黄Ⅱ 辣根过氧化物酶 紫外分光光度计 |
Degradation of Orange Ⅱ Dye Catalyzed by Horseradish Peroxidase with H2O2 as the Oxidant |
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| WANG Yali,MA Mingming.Degradation of Orange Ⅱ Dye Catalyzed by Horseradish Peroxidase with H2O2 as the Oxidant[J].Journal of Ningxia Teachers College,2013(6):55-58,73. |
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| Authors: | WANG Yali MA Mingming |
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| Institution: | ( College of Chemistry and Chemical Engineering, Ningxia Nornal University, Guyuan Ningxia 756000 ) |
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| Abstract: | Abstract This paper studied how pH, temperature, reaction time, H202 concentration, initial concentration of dye would af- fect on the horseradish peroxidase catalytic hydrogen peroxide oxidation degradation of the orange Ⅱdye solution degradation. Re- sults showed that the orangeⅡ dye reached the most ideal deeolorization rate of 83.7% when pH was 2, the temperature was 43 ℃, the reaction time was 12 min,H202 dosage was 2.0 mmol· L-1 and when the initial dye concentration was of 0.8 mmol· L 1. |
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| Keywords: | Azo dyes Orange Ⅱ Horseradish peroxidase UV spectrophotometer |
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