竹叶总黄酮提取工艺研究

探讨用醇提法提取竹叶中黄酮类成分的最佳工艺,为竹叶的开发利用提供理论依据。以竹叶为试材,以乙醇为溶剂,采用分光光度法对竹叶中的黄酮类化合物的含量进行测试。通过单因素实验研究乙醇浓度、料液比、提取温度、提取时间对总黄酮提取率的影响,再采用正交试验法优化提取总黄酮的最佳工艺条件。单因素实验和正交试验表明:影响黄酮提取率的因素主次顺序是:料液比>乙醇浓度>提取温度>提取时间。最佳实验条件为料液比1:20,60%的乙醇,60℃提取4h,竹叶中黄酮的含量为7.88mg.g-1。

Studies on the Extraction Process of Total Flavonoids in Bamboo Leaves

The study aimes to investigate the optmium extraction technology for flavonoid compounds from the bamboo leaves and provides a theoretical basis for the utilization of bamboo leaves.With bamboo leaves as the tested material and ethanol as the solven,the content of total flavonoids in bamboo leaves is detected by visible spectrophotometry.The effects of ethanol concentration,ratio of sample with ethanol solution,extraction temperature,extraction time on the extraction yield of flavonoids are studied through the single factor test and the optmium technology conditions of extracting the total flavonoids are optmized by using the orthogonal experiment.Single factor test and orthogonal tests show that the factors that affected the extraction yield of flavonoid are in the order of ratio of sample with ethanol solution > alcohol concentration > extraction temperature > extraction time.The optimal determination conditions as follows:the 60 % ethanol is used as impregnant,the ratio of sample with ethanol solution 1:20,the total flavonoids are extracted at 60 ℃ for 4 hours.The average content of total flavonoids in bamboo leaves is 7.88 mg·g-1 under the optimal determination conditions.