| 不同条件对胰蛋白酶水解酪蛋白水解度的影响 |
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| 引用本文： | 于传瑞,杜开书,吴菲,申文鹏,娄伟强,丁凯,李淑梅.不同条件对胰蛋白酶水解酪蛋白水解度的影响[J].河南职业技术师范学院学报,2013(6):6-8. |
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| 作者姓名： | 于传瑞 杜开书 吴菲 申文鹏 娄伟强 丁凯 李淑梅 |
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| 作者单位： | 河南科技学院,河南新乡453003 |
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| 基金项目： | 河南科技学院大学生创新实验项目（2011CXSY36） |
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| 摘 要： | 研究了不同温度和水解时间以及不同浓度的SDS对胰蛋白酶水解酪蛋白水解度的影响.结果表明：在酪蛋白浓度为4%、加酶量2 500 U/g、pH为7的条件下,选择温度为50℃,水解时间为80 min,酪蛋白的水解效果较佳,水解度为7.30%;同等条件下向此体系加入适量的SDS后,使SDS终浓度为0.001 28 g/mL,水解度提高为13.50%.
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| 关 键 词： | 酪蛋白 胰蛋白酶 SDS 水解度 |
Effect of different conditions on trypsin hydrolysis of casein hydrolysis degree |
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| Yu Chuanrui,Du Kaishu,Wu Fei,Shen Wenpeng,Lou Weiqiang,Ding Kai,Li Shumei.Effect of different conditions on trypsin hydrolysis of casein hydrolysis degree[J].Journal of Henan Vocation-Technical Teachers College,2013(6):6-8. |
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| Authors: | Yu Chuanrui Du Kaishu Wu Fei Shen Wenpeng Lou Weiqiang Ding Kai Li Shumei |
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| Institution: | (Henan Institute of Science and Technology,Xinxiang 453003,China) |
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| Abstract: | Effects of different temperature and time of hydrolysis and different concentrations of SDS to trypsin hydrolysis of casein hydrolysis degree were studied in experiment.The results showed that the best effect of hydrolysis of casein are conditions of casein concentration 4%,the amount of enzyme 2 500 U/g, temperature 50 ℃,reaction time of 80 min,the degree of hydrolysis is 7.30%.After joining the moderate SDS under the same conditions,the final concentration of SDS is 0.001 28 g/mL, the degree of hydrolysis can be increased to 13.50%. |
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| Keywords: | casein trypsin SDS degree of hydrolysis |
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