| 紫外光谱法研究2-羟甲基苯并咪唑与DNA的相互作用 |
| |
| 引用本文： | 郁有祝,宋群立,郭玉华.紫外光谱法研究2-羟甲基苯并咪唑与DNA的相互作用[J].安阳工学院学报,2016,15(6). |
| |
| 作者姓名： | 郁有祝 宋群立 郭玉华 |
| |
| 作者单位： | 安阳工学院化学与环境工程学院,河南安阳,455000;许昌幼儿师范学校,河南许昌,461700 |
| |
| 摘 要： | 用紫外光谱法和黏度法研究2-羟甲基苯并咪唑与DNA的相互作用，考察离子强度对两者相互作用的影响。结果表明：在pH=7.40的Tris-HCl缓冲溶液中，DNA使2-羟甲基苯并咪唑的紫外吸收光谱减色且红移，测得2-羟甲基苯并咪唑与DNA的结合常数为5.2×107 L?mol-1。随2-羟甲基苯并咪唑浓度增大，DNA黏度增大，NaCl浓度增加，DNA-2-羟甲基苯并咪唑体系吸光度无明显变化，2-羟甲基苯并咪唑以嵌插作用方式与DNA结合。
|
| 关 键 词： | 2-羟甲基苯并咪唑 DNA 紫外光谱 黏度 相互作用 |
Studies on the Interaction between 2-(hydroxymethyl) Benzimidazole and DNA by UV Spectrophotometry |
| |
| YU Youzhu,SONG Qunli,GUO Yuhua.Studies on the Interaction between 2-(hydroxymethyl) Benzimidazole and DNA by UV Spectrophotometry[J].Journal of Anyang Institute of Technology,2016,15(6). |
| |
| Authors: | YU Youzhu SONG Qunli GUO Yuhua |
| |
| Abstract: | Purpose:the interaction between 2-(hydroxymethyl)benzimidazole and DNA was studied by UV spec?trophotometry and viscosity measurements, and influence of ion strength on the binding of 2-(hydroxymethyl) benzimidazole with DNA was also investigated. Results:DNA resulted in some decrease and red shift in the ab?sorption of 2-(hydroxymethyl)benzimidazole. The binding constant for 2-(hydroxymethyl)benzimidazole with DNA was 5.2 × 107 L?mol-1. The relative viscosity of DNA increased in the presence of 2-(hydroxymeth yl)benz?imidazole, there was no influence of ion strength on the absorbance of 2-(hydroxymethyl)benzimidazole-DNA. Conclusion:the binding of 2-(hydroxymethyl)benzimidazole with DNA was an intercalation binding. |
| |
| Keywords: | 2-(hydroxymethyl)benzimidazole DNA UV spectrophotometry viscosity interaction |
| 本文献已被 万方数据 等数据库收录！ |
