组织化学技术在体育科研中的应用

从普通组织化学、酶组织化学、免疫组织化学、原位杂交组织化学及定量组织化学等方面对国内外组织化学技术在体育科研中的应用研究进行了综述.随着现代生物化学和分子生物学的飞速发展,在体育科学研究中普通组织化学方法逐渐退出而被其它更高效、精确的检测手段如免疫学方法及定量组织化学方法等技术代替.其中,流式细胞技术、激光扫描共聚焦显微镜技术和免疫电镜技术等先进组织化学技术在体育科研中日益显示出其巨大的应用潜力,并为此方面的研究提供丰富、稳定、可靠、准确的研究平台.

Application of histochemical techniques in sports researches

The authors gave an overview of domestic and foreign researches on the application of histochemical techniques in sports researches from such aspects as common histochemistry, enzyme histochemistry, immunohistochemistry, in situ hybridization histochemistry and quantitative histochemistry. With the rapid development of modem biochemistry and molecular biology, common histochemical methods used in sports researches are gradually becoming obsolete and replaced by other test means that are more efficient and accurate, such as immunological methods and quantitative histochemical methods, in which advanced histochemical techniques such as flow cy- tometry technique, laser scanning confocal microscope technique and immunohistochemical electron microscope technique are ever increasingly showing their tremendous potentials for application in sports researches, and providing a comprehensive, stable, reliable and accurate research platform for researches in this area.