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川滇桤木果实中总黄酮的含量测定
引用本文:杨晓珍,尹暗梅,杨永寿.川滇桤木果实中总黄酮的含量测定[J].大理师专学报,2013(12):9-11.
作者姓名:杨晓珍  尹暗梅  杨永寿
作者单位:大理学院药学与化学学院,云南大理671000
基金项目:大理学院大学生科研基金资助项目(DXSKY201213)
摘    要:目的:建立川滇桤木果实中总黄酮的含量测定方法。方法:采用紫外-可见分光光度法,以芦丁为对照品测定川滇桤木果实中总黄酮的含量。结果:芦丁在0.19~0.95mg/mL范围内呈良好的线性关系,A=1.0826C+0.0497(r=0.9996),平均加样回收率为99.6%,RSD为1.09%(n=6)。结论:该方法操作简便、快速、准确、重现性好,可作为川滇桤木果实中总黄酮的含量测定方法。

关 键 词:川滇桤木  总黄酮  紫外一可见分光光度法

Determination of Content of Total Flavones in Alnus ferdinandi-coburgii
YANG Xiaozhen,YIN Lamei,YANG Yongshou.Determination of Content of Total Flavones in Alnus ferdinandi-coburgii[J].Journal of Dali Teachers College,2013(12):9-11.
Authors:YANG Xiaozhen  YIN Lamei  YANG Yongshou
Institution:(College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China)
Abstract:Objective: To establish a method for determining the content of flavonoid in Alnus ferdinandi-coburgii. Methods: The content of flavonoid was detected from Alnus ferdinandi-coburgii by UV-visible method and the rutin was used as control.Results: Rutin was good linear in the range of 0.19 - 0.95 mg/mL :A=1.082 6C+0.049 7(r=0.999 6) the average recovery of 99.6 % and RSD of 1.09 % (n=6). Conclusion: The method is simple, rapid and accurate. It can be used for determining the content of total flavones in A lnus fe rdinandi-cob urgii.
Keywords:A lnus ferdinandi-coburgii  flavonoid  UV-visible method
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