乳腺癌原代细胞培养及化疗药敏的研究

目的：通过对乳腺癌原代细胞培养。检测化疗药物对癌细胞的抑制率。探讨乳腺癌不同组织学类型、年龄及淋巴结转移情况对化疗药物的敏感性。方法：收集109例手术切除新鲜乳腺癌标本．进行原代细胞培养。采用四甲基偶氮唑盐（MTT）法检测癌细胞对三组抗癌药物的敏感性。结果：乳腺癌的敏感率由高到低依次为CAF（33．0％）、TA（31．2％）和CMF（26．6％）。无论年龄、组织学类型及是否有淋巴结转移各组间比较其敏感性均无显著差异（P〉0．05）。结论：1．MTT法对乳腺癌治疗时选择化疗药物具有重要价值；2．乳腺癌细胞对CAF、TA、CMF敏感。

Study on the Primary Culture and Chemotherapeutic Drug Sensitivity of Breast Cancer Cells

Objective: To investigate the chemosensititivity of three vinds chemotherapeutic drug groups on different the histologic types,age and lymph node metastasis status of breast carcinoma by the primary culture of breast cancer cell.Methods: Fresh samples from 109 patients with breast cancer were obtained during operation.The breast cancer cells were cultured in primary to test the 3 groups drugs sensitivity by MTT method.Results:The sensitivity rates of the breast cancer were(from high to low) CAF(33.0%),TA(31.2%) and CMF(26.6%).There was no significant difference in sensitivity(P>0.05) among the age groups,the histologic type groups and the lymph node metastasis groups.Conclusion:To choose chemotherapeutic drugs by MTT assay has important significance in treating breast cancer.The breast cancer cells are sensitive to CAF,TA and CMF,but no significance.