不同处理方法对柑桔内生细菌发酵液抗菌活性的影响

从柑桔健康组织中分离得到的内生细菌“重桔1号”，制成发酵液，发酵液经不同方法处理后进行抑菌实验，用油菜菌核菌做指示菌，以灭菌水为对照。实验结果表明，发酵液直接用0．45μm过滤器过滤或发酵液经过90℃，60min高温处理和紫外线照射30min处理效果都比较显著，因此我们不但可以证明柑桔内生细菌的代谢产物中含有抑菌物质，而且找到了判断拮抗菌能否产生抗菌产物的快速而有效的方法，为药源菌株的筛选提供实验依据。

An Impact of Different Method over Endogenous Orange Bacterium Fermentation Liquid Antibiosis Activity

The result the liquid the experiment bear fruit being indicated , fermenting is handled directly with 0.45 mum filter fliteration or the liquid fermenting by that 90 -C 60 min high-temperature treatment and ultraviolet rays irradiate 30 rains has been all comparatively notable , we contain bactcriostat matter therefore in the supersession outcome being able to testify the endogenous bacterium of orange not only, and have found the speedy but effective method whether the antibiosis can produce antibiosis outcome＇s to judge that Jie, preparation by screening of source bacterial strain provides the experiment basis to medicine.