同时分离测定蜂蜜中13种喹诺酮类药物残留

实验采用VP-ODS色谱柱(150 mm×4.6 mm,5.0μm),以5%甲醇-10%乙腈-85%30 mM磷酸缓冲液(pH3.0,体积比)为流动相;流速为1.0 mL/min,检测波长为285 nm.该方法对13种喹诺酮类药物的线性范围为0.03～100μg/mL,最低检测限为0.001～0.03μg/mL;样品萃取回收率均大于64%,方法简单,准确,为食品中喹诺酮类药物的残留检测提供了一种有效的分析方法.

Simultaneous Separation and Determination of Thirteen (Fluoro) Quinolones in Honey Sample by Solid-phase Extraction and Reversed-phase High-performance Liquid Chromatography with UV-vis Detection

The good chromatographic separation was achieved on a VP-ODS column(150mm×4.6mm,5.0μm) through variation of the mobile phase additive combinations,which consisted of 5% methanol,10% acetonitrile and 85% 30mM phosphate buffer solution(pH 3.0) at a flow rate of 1.0mL/min under an optimization isometric program,allowed to accomplish the chromatographic separation in 46min.UV detector wavelength was set at 285nm.The method was linear over the concentration range of 0.03 ～ 30.0μg/mL or 0.10 ～ 100μg/mL for thirteen(fluoro)quinolones,and the detection limits were between 0.001 ～ 0.03μg/mL.The solid-phase extraction recovery of the thirteen(fluoro)quinolones from honey sample were beyond 64%.This method will provide the method for the multiresidue determination of drugs in food.