| 高效液相色谱法测定草莓中维生素C含量 |
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| 引用本文： | 王艳颖,姜国斌,胡文忠,范圣第,姜波.高效液相色谱法测定草莓中维生素C含量[J].大连大学学报,2006,27(2):21-22,26. |
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| 作者姓名： | 王艳颖 姜国斌 胡文忠 范圣第 姜波 |
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| 作者单位： | 大连民族学院,生命科学学院,辽宁,大连,116600 |
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| 摘 要： | 目的:建立一种高效液相色谱测定草莓中维生素C含量的新方法.方法:采用HYPERSIL-C18色谱柱,0.1%低浓度的草酸作流动相,流速为1.0 mL/m in,检测波长为254 nm.结果:维生素C在浓度为20~100 mg/L范围内有良好的线性关系,线性回归方程为y=9 361.6x-11 192,相关系数为0.999 3,方法回收率为97.4~102.1%,相对标准偏差小于3%.结论:本测定方法简便,快速,准确,稳定;适合于测定其它水果和蔬菜中维生素C的含量.
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| 关 键 词： | 高效液相色谱 草莓 维生素C |
| 文章编号： | 1008-2395（2006）02-0021-03 |
| 收稿时间： | 2006-03-02 |
| 修稿时间： | 2006年3月2日 |
Determination of vitamin C in strawberry by high performance liquid chromatography |
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| WANG Yan-ying,JIANG Guo-bin,HU Wen-zhong,FAN Sheng-di,JIANG Bo.Determination of vitamin C in strawberry by high performance liquid chromatography[J].Journal of Dalian University,2006,27(2):21-22,26. |
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| Authors: | WANG Yan-ying JIANG Guo-bin HU Wen-zhong FAN Sheng-di JIANG Bo |
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| Abstract: | Objective] A new method of detecting the conent of vitamin C in the strawberry by using HPLC is established. Methods] A HYPERSIL- C18 column is used and the flow phase is low concentration 0. 1% oxalic acid,the flow rate is 1.0 mL/min and the detection waveleng is 254 nm in the HPLC system. Results ] The standard working curve of vitamin C has a good linear relation in the concentration range from 20 - 100 mg/L,the linear equation is y = 9 361.6 x - 11 192 and the related coefficient is 0. 999 3. The recoveries are 97.4 - 102. 1% and the relatively standard deviation is below 3%. Conclusions] This method is simple and fast and exact and steady and suitable to detect the conent of vitamin C in other fruit and vegetable. |
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| Keywords: | HPLC strawberry vitamin C |
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