基于普鲁士蓝／L-半胱氨酸构建甲胎蛋白免疫传感器的研究

通过静电吸附作用将普鲁士蓝（PB）固定在玻碳电极（GCE）表面，再依次电沉积L半胱氨酸（L—Cys）、氯金酸（HAuCl4）．通过氯金酸与甲胎蛋白抗体（anti—AFP）中氨基的键合作用，将抗体固定在电极表面，最后用牛血清白蛋白（BSA）封闭电极表面的非特异性吸附位点，从而成功制备了一种新型的电流型甲胎蛋白免疫传感器．实验利用循环伏安法对电极的制备过程及性能进行了表征，结果表明该免疫传感器对AFP有很好的电流响应，其线性范围为0．01～200．0ng／mL，检出限为0．003ng／mL．该实验方法操作简便，制得传感器灵敏度高，实现了对AFP的定量分析．

Study on the AFP Immunosensor Based on the PB and L-Cys

First of all,a layer of Prussian blue is fixed on the surface of glassy carbon electrode(GCE).And then L-Cys,HAuCl4 are electrolytically deposited in turn.Through the bonding effect of HAuCl4 and an anti-AFP,the antibody will be fixed on the electrode surface.Finally,bovine serum albumin(BSA) was applied to block the non-specific adsorption sites of the immunosensor,thus a new AFP Immunosensor comes successfully into shape.The preparation process of the electrode and its performance are then subjected to characterization by cyclic voltammetry.The findings show: the said sensor exhibits a high response sensitivity to electric current with a linear range of 0.01~200.0 ng/mL and the detection limit of 0.003 ng/mL.The method is easy to operate and the sensor thus made is highly sensitive,which makes the quantitative analysis of AFP a reality.