| 基于Cu2＋和碳纳米管纳米复合物的过氧化氢生物传感器 |
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| 引用本文： | 杨怀成,曾金祥,莫绪.基于Cu2＋和碳纳米管纳米复合物的过氧化氢生物传感器[J].桂林师范高等专科学校学报,2013,27(2):160-163,167. |
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| 作者姓名： | 杨怀成 曾金祥 莫绪 |
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| 作者单位： | 1. 桂林师范高等专科学校化学与工程技术系,广西桂林,541002
2. 江西中医学院,江西南昌,330004 |
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| 摘 要： | 将Cu2＋和碳纳米管（MWNTs）固定于脱氧核糖核酸（DNA）和聚丙烯胺（PAA）中形成MWNTs／Cu2＋-DNA／PAA纳米复合物，并用此纳米复合物构建了一种新的过氧化氢生物传感器。结果表明，在pH=5．0的磷酸盐缓冲溶液中，所制备的生物传感器对过氧化氢的线性范围为5．0μmol／L--4mmol／L，检测下限为2．5μmol／L（S／N=3）。这种新型传感器的优越性在于它可以克服天然酶易失活、不稳定的缺点而具有良好的重现性与稳定性。
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| 关 键 词： | 过氧化氢 生物传感器 纳米复合物 |
Hydrogen Peroxide Biosensor Based on Cu2+ and Carbon Nanotube Modified Glod Electrode |
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| Yang Huaicheng
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Zeng Jinxiang
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Mo Xuchuan.Hydrogen Peroxide Biosensor Based on Cu2+ and Carbon Nanotube Modified Glod Electrode[J].Journal of Guilin College of Education,2013,27(2):160-163,167. |
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| Authors: | Yang Huaicheng Zeng Jinxiang Mo Xuchuan |
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| Institution: | Yang Huaicheng, Zeng Jinxiang , Mo Xuchuan (1. Department of Chemistry, Guilin Normal College, Guilin, Guangxi 541002, China J iangxi University of Traditional Chinese Medicine, Nanchang , J iangxi 330004, China) |
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| Abstract: | A novel amperometric hydrogen peroxide biosensor was fabricated by integrating the Cu2＋ and multi --wall carbon nanotubes （MWNTs） into a DNA and poly（allylamine） （PAA） complex to form a new MWNTs/Cu2＋-DNA/PAA nanocomposites. In air--saturated PBS solution （pH 5.0）, the biosensor showed a wide dynamic range of 5. 0m M-14m M hydrogen peroxide, and the linear ranges were 5. 0μmol/L - 4mmol/L with a detection limit of 2.5μmol/L estimated at a signal--to--noise ratio of 3. Furthermore, such sensor can overcome the disadvantages of native enzyme--based biosensors since it does not need native en- zyme and it exhibits good reproducibility and stability. |
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| Keywords: | , hydrogen peroxide,amperometric biosensor,nanocomposite |
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