| 裂殖壶菌油脂提取方法的研究 |
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| 引用本文： | 马建设,;韩方方,;叶海仁,;周茂洪,;黄可新,;赵肖为.裂殖壶菌油脂提取方法的研究[J].温州大学学报(社会科学版),2009(6):21-24. |
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| 作者姓名： | 马建设 ;韩方方 ;叶海仁 ;周茂洪 ;黄可新 ;赵肖为 |
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| 作者单位： | [1]温州医学院药学院; [2]温州大学生命与环境科学学院,浙江温州325035 |
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| 基金项目： | 浙江省科技计划项目（2007C22G2250004） |
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| 摘 要： | 比较了有机溶剂提取法、酸热法和超声波提取法对于裂殖壶菌油脂的提取能力．酸热法和超声波提取法的提取得率高于有机溶剂提取法．当氯仿和甲醇以1：1的体积比混合时，油脂提取得率最高．当氯仿和甲醇以2：1的体积比混合时，提取所得油脂中的DHA含量最高．但总提取得率还是以氯仿和甲醇的体积混合比为1：1时为最佳．
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| 关 键 词： | 裂殖壶菌 油脂 酸热法 有机溶剂提取法 超声波提取法 |
Study on Methods of Lipid Extraction from Schizochytrium |
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| Institution: | MA Jianshe, HAN Fangfang, YE Hairen, ZHOU Maohong, HUANG Kexin, ZHAO Xiaowei (1. Pharmacy School, Wenzhou Medical College; 2. College of Life and Environmental Sciences, Wenzhou University, Wenzhou, China 325035) |
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| Abstract: | Comparative studies among 3 different methods of lipid extraction from Schizochytrium （acidheating, organic solvent and ultrasonic extraction） were carried out to evaluate their lipid extraction efficiency The efficiency of acid-heating extraction or ultrasonic extraction was higher than that of the organic solvent extraction. The lipid extraction efficiency was highest when chloroform and methanol were mixed at the ratio of 1：1 （v/v）. The content of DHA in the extracted lipids was highest when chloroform and methanol were mixed at the ratio of 2：1 （v/v）. According to the experiments, the optimum lipids extraction efficiency can be achieved when chloroform and methanol are mixed at the ratio of 1：1 （v/v） |
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| Keywords: | Schizochytrium Lipid Acid-heating Extraction Organic Solvent Extraction Ultrasonic Extraction |
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