纤维素降解过程中金属离子作用的研究

用微量量热法研究了7种金属离子及几种混合离子对纤维素酶降解纤维素反应的影响.实验结果表明,在pH=4.60,T=310K时,相同浓度的离子中Li+、Na+、K+、Ni2+、Zn2+、Co2+离子均表现出抑制作用,其中Li+抑制作用显著;Cu2+有明显的激活作用.当Li+、Na+、K+3种离子混合共同作用于反应时,表现出激活作用;当Ni2+、Cu2+、Zn2+3种离子,Co2+、Ni2+2种离子混合共同作用于反应时,表现出抑制作用.

Study of effect of Metal Ions on Cellulose Degradation

In this paper the effect of seven kinds of metal ions and the mixed ones on cellulose degradation by cellulase was studied by microcalorimetry. The results showed: When T=310 K pH=4.60 and the concentration of metal ions was the same,Li~+,Na~+,K~+,Ni~(2+),Zn~(2+),Co~(2+)had some inhibition on the cellulase,among which Li~+ had obvious inhibition. Contrarily,cellulase was activated by Cu~(2+). Cellulase was activated by the mixture of Li~+,Na~+,K~+. While the mixture of Ni~(2+),Cu~(2+),Zn~(2+) and that of Co~(2+),Ni~(2+)had inhibitive effect on cellulase.