运动后血清和骨骼肌HSP60的关系及对骨骼肌的保护作用

目的探讨运动后血清、骨骼肌HSP60含量变化、相关关系及其对骨骼肌的保护作用。方法26名成人(15男11女)进行60 min的功率脚踏车运动,运动负荷为70%的最大摄氧量(VO2max)。于运动开始30 min,结束后即刻、1、2、8、244、8 h采集血样、股外侧肌活检,检测HSP60、肌酸激酶骨骼肌同工酶(CK-MM)含量。结果血清HSP60和骨骼肌内HSP60含量变化是一致的,血清CK的变化相比HSP60的变化是上升的晚,下降的早。结论剧烈运动后HSP60在血清及骨骼肌内含量增加,血清HSP60可能是由骨骼肌细胞分泌。

HSP60 Relationship Between Serum and Skeletal Muscle Post- Exercise and Its Protecting Role on Skeletal Cells

Object:to study HSP60 relationship between serum and skeletal muscle and protection on skeletal muscle.Method:26 humans(15 males,11 female) ran on a treadmill for 60 minutes at a workload corresponding to 70% of their peak oxygen consumption.Blood was sampled from a forearm vein at rest(R),30 minutes during exercise,immediately post exercise,1,2,8,24 and 48 hours after exercise.These samples were analyzed for serum Hsp60 protein and CK.Muscle biopsies were also obtained from the vastus lateralis at the time of blood sampling and analyzed for Hsp60 protein expression.Results:HSP60 in serum is accordant with content in the skeletal muscle.Compared with the CK variance,HSP60 increased earlier and decreased late.Conclusions: HSP60 in serum and skeletal muscles increased after an acute exercise,HSP60 in serum may be secreted by cells of skeletal muscles and these proteins may play an important role in protection the skeletal muscles.