| 利用CTAB法提取蝴蝶兰的基因组DNA |
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| 引用本文： | 范永山,谷守芹,李莉.利用CTAB法提取蝴蝶兰的基因组DNA[J].保定师专学报,2005,18(4):57-59. |
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| 作者姓名： | 范永山 谷守芹 李莉 |
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| 作者单位： | [1]唐山师范学院生物系,河北唐山063000 [2]保定师范专科学校生物系,河北保定071000 |
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| 基金项目： | 河北省科学技术厅资助（05547007D-2） |
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| 摘 要： | 利用CTAB法对蝴蝶兰的根、叶、花等不同部位进行基因组DNA的提取，然后分别用紫外分光光度法和琼脂糖凝胶电泳法检测DNA的质量，结果表明，3个部位都可以获得较完整的基因组DNA，电泳条带清晰，无拖尾现象．其中根部提取的DNA纯度最高，OD260／OD280为1．6545；其次为叶，OD260／OD280为1．5351；花的DNA纯度最低，仅为1．3820．
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| 关 键 词： | 蝴蝶兰 DNA提取 CTAB裂解法 |
| 文章编号： | 1008-4584（2005）04-0057-03 |
| 收稿时间： | 09 29 2005 12:00AM |
| 修稿时间： | 2005-09-29 |
Genomic DNA Extraction from Phalaenopsis hybrid by CTAB Method |
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| FAN Yong-shan, GU Shou-qin, LI li.Genomic DNA Extraction from Phalaenopsis hybrid by CTAB Method[J].Journal of Baoding Teachers College,2005,18(4):57-59. |
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| Authors: | FAN Yong-shan GU Shou-qin LI li |
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| Institution: | 1. Department of Biology ,Tangshan Teachers College, Tangshan 063000, China; 2. Department of Biology ,Baoding Teachers College, Baoding 071000, China |
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| Abstract: | Genomic DNA from the roots,leaves and flowers of Phalaenopsis hybrid was extracted by CTAB(Cetyl-trimethyl Ammonium Bromide) method.The results of DNA quality detection by agarose gel electrophoresis and ultraviolet spectrophotometry had shown that intact genomic DNA could be acquired from the three parts of the flower,but the DNA purity was not ideal for strict molecular biological experiments.The ratio of OD260 and OD280 ranged from 1.38 to 1.65.The DNA from roots had relatively the highest quality,with OD260/OD280 of 1.654 5,followed by the DNA from leaves,with OD260/OD280 of 1.535 1.The DNA from flowers had the lowest quality,with OD260/OD280 of 1.3820. |
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| Keywords: | Phalaenopsis hybrid DNA extraction CTAB method |
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