| 人工养殖长吻不同组织3种同工酶的研究 |
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| 引用本文： | 刘鸿艳,郑宗林,解琦.人工养殖长吻不同组织3种同工酶的研究[J].凯里学院学报,2008,26(3). |
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| 作者姓名： | 刘鸿艳 郑宗林 解琦 |
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| 作者单位： | 西南大学水产系,重庆,荣昌,402460 |
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| 摘 要： | 采用聚丙烯酰胺垂直梯度凝胶电泳法对养殖长吻鱼危的8种组织(心脏、肝、脾、脑、眼、肌肉、肾、黏液)进行了3种同工酶(LDH、ADH、POD)的初步研究,结果表明,长吻鱼危的3种同工酶系统具有明显的组织特异性:LDH同工酶在各个组织中均表达为5条酶带,但活性呈现组织差异,Ldh-A4在肌肉中优势表达,Ldh-B4在心脏、肾脏中优势表达;ADH同工酶组织特异性显著,Adh1、Adh2为肝脏特异性酶带,Adh9为心脏特异性带;除眼、肌肉和黏液无POD同工酶表达外,其余组织均表达为1条酶带,脾脏为优势表达组织.
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| 关 键 词： | 同工酶 组织特异性 人工养殖长吻(鱼危) |
Study on three isozymes expressed in various tissues of raised leiocassis iongirostris |
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| LIU Hong-yan,ZHENG Zong-lin,XIE Qi.Study on three isozymes expressed in various tissues of raised leiocassis iongirostris[J].Journal of Southeast Guizhou National Teachers College,2008,26(3). |
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| Authors: | LIU Hong-yan ZHENG Zong-lin XIE Qi |
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| Institution: | LIU Hong-yan,ZHENG Zong-lin,XIE Qi(Department of Fisheries of Southwest University,Rongchang,Chongqing,402460,China) |
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| Abstract: | In the studies, 3 isozymes expressed in 8 tissues (that is heart, liver, spleen, brain, eye, muscle, kidney and slime) of raised Leiocassis longirostris were investigated by polyacrylamid gel electrophoresis (PAGE). The result showed that isozymic expression in raised L. longirostris has tissue-specificity. There were 5 LDH bands found in the tissues with different activity, Ldh-A4 isozyme expressed dominantly in the muscle, while-B4 was found in the heart and kidney with dominate activity. ADH was pigmente... |
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| Keywords: | isozymes tissue-specificity raised Leiocassis longirostris |
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