| 马拉松运动对人体外周血细胞DNA损伤和氧化应激的影响 |
| |
| 引用本文： | 刘晓莉,苏美华,沈飞,乔德才.马拉松运动对人体外周血细胞DNA损伤和氧化应激的影响[J].北京体育大学学报,2009(7). |
| |
| 作者姓名： | 刘晓莉 苏美华 沈飞 乔德才 |
| |
| 作者单位： | 北京师范大学体育与运动学院; |
| |
| 基金项目： | 国家自然科学基金资助项目(30771050) |
| |
| 摘 要： | 目的:观察马拉松运动后不同时相人体外周血细胞DNA的损伤效应及血浆中SOD、GSH和MDA活性的变化,探讨大强度耐力运动引起人体血细胞DNA损伤的机制。方法:选取7名参加2007年北京国际马拉松赛的健康男运动员,分别在运动前一天(CG)、运动后2 h(2 h)2、4 h(24 h)和48 h(48 h)采血抗凝,用单细胞凝胶电泳(SCGE)技术检测人体外周血细胞DNA损伤,并测定血浆中超氧化物歧化酶(SOD)活性、谷胱甘肽(GSH)和丙二醛(MDA)含量。结果:运动后2 h和24 h DNA损伤程度显著高于安静水平,2 h显著高于24 h;48 h基本恢复至安静水平。2 h血浆中SOD活性、GSH和MDA含量均较安静水平显著升高;24 h SOD和MDA仍显著高于安静水平,而GSH水平则无显著差异;48 h SOD、GSH和MDA均较安静水平无显著差异。结论:马拉松运动对人体外周血细胞的DNA损伤具有明显的诱发作用,且存在一定的时序性特征。运动性氧应激是导致人体外周血细胞DNA损伤的原因之一。
|
| 关 键 词： | DNA损伤 氧化应激 外周血细胞 马拉松 |
The Effect of Marathon Running on DNA Damage and Oxidative Stress in Human Peripheral Blood Cell |
| |
| LIU XIAO-li,SU MEI-hua,SHEN Fei,QIAO De-cai.The Effect of Marathon Running on DNA Damage and Oxidative Stress in Human Peripheral Blood Cell[J].Journal of Beijing Sport University,2009(7). |
| |
| Authors: | LIU XIAO-li SU MEI-hua SHEN Fei QIAO De-cai |
| |
| Institution: | LIU XIAO-li,SU MEI-hua,SHEN Fei,QIAO De-cai (Physical Education , Sports College,Beijing Normal University,Beijing100875,China) |
| |
| Abstract: | Objective: to investigate the DNA damage and the level of anti-oxidative status on human peripheral blood cell in different time after Marathon running,and discuss the mechanism of DNA damage induced by endurance exercise.Method: We select seven healthy men athletes,who participated in International Marathon Match in 2007.The subjects are required to have a test at the time of twenty-four hours' pre-exercise,also 2 hours,24 hours and 48 hours after exercise. We investigate the DNA damage on human peripheral... |
| |
| Keywords: | DNA damage oxidative stress peripheral blood cell marathon running |
| 本文献已被 CNKI 等数据库收录！ |
|
