| 枇杷果实液泡膜V-ATPase D亚基基因的克隆及表达 |
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| 引用本文： | 谢晓清,陈发兴,赖钟雄.枇杷果实液泡膜V-ATPase D亚基基因的克隆及表达[J].莆田学院学报,2013(5):51-54. |
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| 作者姓名： | 谢晓清 陈发兴 赖钟雄 |
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| 作者单位： | 福建农林大学园艺植物生物工程研究所／亚热带果树研究所,福建福州350002 |
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| 基金项目： | 福建省自然科学基金资助项目（2010J01071,B0310008）;福建省教育厅资助项目（JA05231） |
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| 摘 要： | 提取枇杷果实总RNA,根据RNAseq数据库查找得到的(液泡膜质子泵V-ATPase)基因片段序列11个。设计引物,采用RACE技术,获得质子泵V-ATPase cDNA的全长,对测序结果进行分析,并将序列在NCBI上进行blast比对,结果显示核苷酸序列和氨基酸同源性均在80%以上,为质子泵酶基因V-ATPase D亚基基因。经qPCR分析表明,该基因对枇杷果实有机酸具有明显调控作用。
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| 关 键 词： | 枇杷 有机酸 液泡膜质子泵 V-ATPase |
The Clone and Expression of V-ATPase Subunit D Gene from Loquat Fruit |
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| XIE Xiao-qing,CHEN Fa-xing,LAI Zhong-xiong.The Clone and Expression of V-ATPase Subunit D Gene from Loquat Fruit[J].journal of putian university,2013(5):51-54. |
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| Authors: | XIE Xiao-qing CHEN Fa-xing LAI Zhong-xiong |
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| Institution: | (Institute of Horticultural Biotechnology/Institute of Subtropical Pomology, Fujian Agriculture and Forestry University, Fuzhou Fujian 350002, China ) |
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| Abstract: | Total RNA was extracted from loquat fruit. The primers were designed according to RNAseq database of loquat V-ATPase gene fragment. In this study, a full length cDNA of V-ATPase gene was cloned by the RACE technology. The nucleotide and protein sequencing analysis indicated that the cloned fragment was V-ATPase subunit D which was more than 80 % identical to the published sequences. According to the analysis of qPCR, the V-ATPase gene and Organic acids had good correlation in loquat fruit. |
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| Keywords: | loquat organic acid vacuolar proton pumps V-ATPase |
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