| 中小强度有氧运动后大鼠右心室肌蛋白质组差异表达的研究 |
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| 引用本文： | 徐哲,史绍蓉,谭军,郭远盘,王娟.中小强度有氧运动后大鼠右心室肌蛋白质组差异表达的研究[J].北京体育大学学报,2010(10). |
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| 作者姓名： | 徐哲 史绍蓉 谭军 郭远盘 王娟 |
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| 作者单位： | 湖南师范大学体育学院;湖南省人民医院; |
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| 基金项目： | 湖南省科技厅计划项目(2007FJ3075) |
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| 摘 要： | 目的:运用蛋白质组学的理论和方法来研究中小强度有氧运动对大鼠右心室肌蛋白质组的影响。方法:将20只SD雄性大鼠随机分为对照组和运动组(各1组,每组n=10)。采用中小强度运动负荷跑台训练建立运动实验模型,提取右心室肌组织全蛋白样品,采用双向凝胶电泳进行蛋白样品分离。运用ImageMaster 2D Platinum图像分析软件对2-DE胶进行分析,通过图像分析,选取运动后差异表达量≥5倍的蛋白点,用串联飞行时间质谱蛋白质仪(ULGRAFL-FLEX-TOF/TOF)进行质谱鉴定。结果:经过8周运动后,在2-DE图谱上运动组和对照组检测到的蛋白质点分别为(547±18)个和(530±14)个,匹配率分别为(71.34±3.25)%和(70.28±2.38)%。运动后共有58个蛋白质点发生了变化:表达量上调≥2倍的点有33个,下调≥2倍的点有25个,其中上调≥5倍的点有8个,下调≥5倍的点有8个。选择差异表达量≥5倍的蛋白质点为目标蛋白质进行质谱分析,共鉴定出了5个差异表达蛋白质点。结论:在长期中小强度有氧运动后,大鼠右室肌蛋白质组发生了明显的差异性表达,这些差异性表达的蛋白质点为进一步观察在运动应激状态下对心脏...
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| 关 键 词： | 蛋白质组 右心室肌 中小强度有氧运动 双向凝胶电泳 质谱鉴定 |
The Research of Different Expressions of Right Ventricular Muscle Proteome after Moderate and Small Intensity Aerobic Exercise in Rats |
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| XU Zhe,SHI Shao-Rong,TAN Jun,GUO Yuan-Pan,WANG Juan.The Research of Different Expressions of Right Ventricular Muscle Proteome after Moderate and Small Intensity Aerobic Exercise in Rats[J].Journal of Beijing Sport University,2010(10). |
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| Authors: | XU Zhe SHI Shao-Rong TAN Jun GUO Yuan-Pan WANG Juan |
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| Institution: | XU Zhe1,SHI Shao-Rong1,TAN Jun2,GUO Yuan-Pan1,WANG Juan1(1.Physical Department of Hunan Normal University,Changsha 410082,Hunan China,2.People's Hospital of Hunan Province,Changsha 410005,Hunan China) |
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| Abstract: | objective:Proteomic methodology was used to study the influence of Moderate and small intensity aerobic exercise on the right ventricle proteins in rats.Methods:20 rats were divided into rest and exercise groups in random.The exercise group was set up to do treadmill training with medium and small intensity exercise and then killed to extract the total protein of right ventricular muscle for two dimensional gel electrohoresis searation.The spots in different expression volume more than 5 times are selected ... |
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| Keywords: | proteome right ventricular muscle medium and small intensity aerobic exercise two dimensional gel electrophoresis MS/MS |
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