Ebselen 对大强度耐力训练大鼠肾脏NO、NOS 系统及自由基代谢的影响

目的：探讨ebselen对运动训练大鼠肾脏丙二醛、总抗氧化能力、超氧化物歧化酶、谷胱甘肽过氧化物酶以及一氧化氮和一氧化氮合酶表达的影响。方法：SD雄性健康大鼠30只，随机分为3组：安静对照组、运动对照组、运动加药组各10只；后两组进行为期8周递增负荷跑台训练，建立大强度耐力训练模型测定肾脏上述相应指标。结果：用ebselen干预后，相比运动对照组，运动服药组各项指标均有明显改善。丙二醛含量显著降低（P〈0.05），总抗氧化能力显著升高（P〈0.05），谷胱苷肽过氧化物酶活性显著升高（P〈0.05），超氧化物歧化酶活性显著升高（P〈0．05），一氧化氮合酶和诱导性一氧化氮合酶表达有所下降（P〈0.05），NO生成减少（P〈0.05）。结论：ebselen对大强度耐力训练中肾脏产生的自由基有良好的清除作用，可以有效对抗运动造成的肾脏缺血再灌注损伤。

Effect of Ebselen on NO/NOS System and Free Radical Metabolism Induced by High-Intensity and Endurance-Training in the Rat

Objective: To study the effect of ebselen on the expression of MDA, SOD and GSH- Px, total antioxidant capacity, and the expression of NO and NOS system in the trained- rat' s kidney. Methods: Thirty male healthy SD rats were randomized into 3 groups, the control group (A), exercise group (B) and exercise added ebselen group (C) with 10 rats in each group. Group B and C was arranged eight weeks' training and establish the high- intensity and endurance-trained model for 8 weeks. The contents of MDA, SOD, GSH- Px, TAOC, NOS, iNOS and NO were tested in rat' s kidney. Results: After being interfered with ebselen, there were significantly improvement in the various index compared with exercise group, the contents of MDA and NO decreased significantly(P<0.05), the concentration of SOD and GSH- Px increased markedly(P<0.05), the activity of iNOS and NOS decreased notably(P<0.05), the productionof NO reduced obviously (P<0.05). Conclusion: This result indicated that ebselen had a good effect in removing the free radical in rat' s kidney duringhigh- intensity and endurance- trained and ebselen has significant protective effect on kidney of ischemical reperfusion injury.