| 运动对骨骼肌线粒体通透性转换孔及细胞凋亡的影响 |
| |
| 引用本文： | 王冬梅,董军,漆正堂,丁树哲.运动对骨骼肌线粒体通透性转换孔及细胞凋亡的影响[J].西安体育学院学报,2011,28(4):471-475. |
| |
| 作者姓名： | 王冬梅 董军 漆正堂 丁树哲 |
| |
| 作者单位： | 1. 泰山医学院运动人体科学系,山东泰安,271016
2. 华东师范大学体育与健康学院,上海,200241 |
| |
| 摘 要： | 目的观察耐力训练和一次性力竭运动对大鼠骨骼肌线粒体通透性转换孔(mitochondrial permeability transi-tion pore,MPTP)相关因素及细胞凋亡的影响。方法 24只大鼠随机分为3组:对照组(C组)、耐力训练组(T组)和一次性力竭组(E组)。T组进行无负重游泳训练,6次/周,共6周,训练时间从每天45 min逐渐增加到90 min;E组正常笼内饲养6周后进行一次力竭性游泳运动。通过紫外分光光度仪检测MPTP的开放;Western blot检测细胞色素c(Cyt c)的释放;RT-PCR检测Bcl-2和Bax mRNA;双光束紫外分光光度仪检测线粒体Ca2+的转运。结果与对照组相比:(1)T组线粒体吸光度值和Ca2+的转运量无显著性变化,Cyt c的释放量与Bax mRNA的表达显著减少,Bcl-2 mRNA的表达显著增加;(2)E组线粒体吸光度值显著减少,Cyt c释放量及Ca2+转运量显著增加,Bcl-2mRNA表达显著下降,Bax mRNA表达显著增强。结论 耐力运动可能通过上调Bcl-2/Bax基因表达,减少Cyt c释放,稳定MPTP开放,从而减少细胞凋亡;一次性力竭运动可能通过下调Bcl-2/Bax基因表达,MPTP异常开放,增加Cyt c和Ca2+释放,促进细胞凋亡。
|
| 关 键 词： | 线粒体通透性转换孔 细胞色素c Ca2+转运 Bcl-2 Bax |
Effects of Exercises on Skeletal Mitochondrial Permeability Transition Pore and Apoptosis |
| |
| WANG Dong-mei,DONG Jun,QI Zheng-tang,DING Shu-zhe.Effects of Exercises on Skeletal Mitochondrial Permeability Transition Pore and Apoptosis[J].Journal of Xi'an Institute of Physical Education,2011,28(4):471-475. |
| |
| Authors: | WANG Dong-mei DONG Jun QI Zheng-tang DING Shu-zhe |
| |
| Institution: | 1.Department of Sport and Body Science,Taishan Medical College,Tai′an 271016,China; 2.College of Physical Education & Health,East China Normal University,Shanghai 200241,China) |
| |
| Abstract: | Objective: To observe the influence of endurance training and one-off exhaustive exercise on rats skeletal mitochondrial permeability transition pore(MPTP) and apoptosis.Methods: 24 male SD rats were randomly divided into three groups: control group(C group),endurance training group(T group) and one-off exhaustive exercise group(E group).T group undertook swimming training for six weeks,six times per week,from 45mins to 90mins.E group took normal feeding for six weeks,then were killed immediately after one-... |
| |
| Keywords: | mitochondrial permeability transition pore cytochrome c calcium transfer Bcl-2 Bax |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
