应用响应面法优化PEG引发甜玉米种子的条件

将响应面分析法(RSA)应用于提高种子活力条件的筛选。以聚乙二醇(PEG)浓度、引发时间2个因素5个水平的组合对甜玉米种子进行处理,观察种子活力变化,经过SAS软件分析,发现在因素范围内,发芽势、发芽率、发芽指数、平均发芽时间、获利指数均能找到最佳处理组合。进一步进行主成分分析(PCA),发现当PEG浓度13.33%,引发时间24.57 h时,发芽势、发芽率、发芽指数分别为65.21%,74.29%,9.82。

Applying Response Surface Analysis to Select the Conditions of Improving Sweet Corn Seed Vigor by PEG Priming

The experimentation used the response surface analysis(RSA) method to filter the condition of improving the vigor of sweet corn seed,and conclusively acquired favorable affection.The RSA experiment with two factors(PEG concentration and seed priming time) of 14 combinations was performed to investigate the effects on improving seed vigor.By the analyzing of the SAS software,the optimal combinations of the treatments were found in the germination energy,germination percentage,germination index,mean germination time and vigor index,respectively.The data of germination energy,germination percentage and germination index were analyzed by Principal component analysis(PCA),then gained a PCA variable result.By analyzing the PCA variable by RSA,finally gained the best combination conditions of improving seed viability: when PEG concentration was 13.33% and the priming time was 24.57 h,the germination energy was 65.21%,the germination percentage was 74.29%,and the germination index was 9.82.