| 麻风树核糖体失活蛋白的基因组DNA分离及其转化烟草分析 |
| |
| 引用本文： | 罗言云,魏琴,黄明星,徐莺,陈放.麻风树核糖体失活蛋白的基因组DNA分离及其转化烟草分析[J].上海大学学报(英文版),2006,10(5):461-464. |
| |
| 作者姓名： | 罗言云 魏琴 黄明星 徐莺 陈放 |
| |
| 作者单位： | Sichuan Key Lab of Resource Biology and Pharmaceutical Engineering College of Life Sciences Sichuan University Chengdu 610064 P.R. China，Sichuan Key Lab of Resource Biology and Pharmaceutical Engineering College of Life Sciences Sichuan University Chengdu 610064 P.R. China，Department of Biological Engineering Yibin University Yinbin 644007 P.R. China，Sichuan Key Lab of Resource Biology and Pharmaceutical Engineering College of Life Sciences Sichuan University Chengdu 610064 P.R. China，Sichuan Key Lab of Resource Biology and Pharmaceutical Engineering College of Life Sciences Sichuan University Chengdu 610064 P.R. China，Sichuan Key Lab of Resource Biology and Pharmaceutical Engineering College of Life Sciences Sichuan University Chengdu 610064 P.R. China |
| |
| 基金项目： | Project supported by Tenth Five Years Key Program Foundation of the State Science and Technology Commission of China (GrantNo .2002BA901A15) |
| |
| 摘 要： | 1IntroductionRibosome-inactivating proteins(RIPs)occur natu-rally in a variety of higher plant species,and theyfunction by catalytic depurination of a specific aden-osine residue located near the3*terminus of eukary-otic large ribosomal subunit rRNA,preventing EF-2/GTP binding and thereby blocking peptidyl-tRNAtranslocation during protein synthesis1].Many RIPsare potent antiviral and antifungal proteins in vitro2,3],but it may beinsufficient for field application,as com-pared to the ac…
|
| 关 键 词： | 麻风树 烟草 基因内区 核糖体 失活 基因组 |
| 文章编号： | 1007-6417（2006）05-0461-04 |
| 收稿时间： | 2004-09-13 |
| 修稿时间： | 2004-12-17 |
Isolation of a genomic DNA for Jatropha curcas ribosome inactivating protein and its tobacco transformation |
| |
| Yan-yun Luo Ph. D. Candidate,Qin Wei,Ming-xing Huang,Ying Xu,Fang Chen.Isolation of a genomic DNA for Jatropha curcas ribosome inactivating protein and its tobacco transformation[J].Journal of Shanghai University(English Edition),2006,10(5):461-464. |
| |
| Authors: | Yan-yun Luo Ph D Candidate Qin Wei Ming-xing Huang Ying Xu Fang Chen |
| |
| Abstract: | Genomic DNA for Jatropha curcas ribosome inactivating protein (JRIP) was cloned from total DNA of its leaves by polymerase chain reaction (PCR). The no intron character was confirmed. The plant expression vector pBI121-JRIP was constructed by inserting the JRIP gene into pBI121 plasmid. The recombinant Agrobacterium EHA105 strain harboring pBI121-JRIP was constructed by conducting pBI121-JRIP to strain EHA 105. PCR and Southern blotting were carried out, and the results proved that the JRIP gene was integrated into tobacco genome. It might provide a new material for disease resistance tobacco species breeding. |
| |
| Keywords: | Jatropha curcas tobacco transformation intron ribosome inactivating protein |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ |
