ASYMPTOTIC EFFICIENCY IN A PARTLY LINEAR REGRESSION MODEL

ＡＳＹＭＰＴＯＴＩＣＥＦＦＩＣＩＥＮＣＹＩＮＡＰＡＲＴＬＹＬＩＮＥＡＲＲＥＧＲＥＳＳＩＯＮＭＯＤＥＬＬｉａｎｇＨｕａ（ＩｎｓｔｉｔｕｔｅｏｆＳｙｓｔｅｍｓＳｃｉｅｎｃｅ，ＡｃａｄｅｍｉａＳｉｎｉｃａ，Ｂｅｉｊｉｎｇ１０００８０，Ｐ．Ｒ．Ｃｈｉｎａ）Ａ...     

胆甾－4，6－二烯－3－酮的合成

以胆固醇为原料，经二甲亚砜－二环己基碳二亚胺（ＤＭＳＯ－ＤＣＣ）系统选择性氧化，生成胆甾－５－烯－３－酮，产率约３３％．胆甾－５－烯－３－酮经四氯苯醌进一步选择性脱氢生成胆甾－４，６－二烯－３－酮．此化合物是合成１ａ－羟基维生素Ｄ３的重要中间体．     

QUANTUM CHEMICAL STUDIES ON A NEW ORGANOSULFUR REACTION MECHANISM

ＱＵＡＮＴＵＭＣＨＥＭＩＣＡＬＳＴＵＤＩＥＳＯＮＡＮＥＷＯＲＧＡＮＯＳＵＬＦＵＲＲＥＡＣＴＩＯＮＭＥＣＨＡＮＩＳＭＺｈｕＪｉｎ；ＣｈｅｎＹａ－ｌｉｎｇ（ＩｎｓｔｉｔｕｔｅｏｆＣｈｅｍｉｓｔｒｙ，ＡｃａｄｅｍｉａＳｉｎｉｃａ，Ｂｅｉｊｉｎｇ１０００８０...     

THERMODYNAMICS OF MELTING FOR METALS

ＴＨＥＲＭＯＤＹＮＡＭＩＣＳＯＦＭＥＬＴＩＮＧＦＯＲＭＥＴＡＬＳ￥ＨｏｎｇＹｏｎｇ－ｙａｎ（ＩｎｓｔｉｔｕｔｅｏｆＳｏｌｉｄＳｔａｔｅＰｈｙｓｉｃｓ，ＡｃａｄｅｍｉａＳｉｎｉｃａ，Ｈｅｆｅｉ２３００２６）ＷｕＦｅｎｇ－ｍｉｎ（ＺｈｅｊｉａｎｇＵｎｉｖ...     

DETERMINATION OF CYANIDE BY A SPECTROPHOTOMETRIC REVERSED FLOW INJECTION METHOD

ＤＥＴＥＲＭＩＮＡＴＩＯＮＯＦＣＹＡＮＩＤＥＢＹＡＳＰＥＣＴＲＯＰＨＯＴＯＭＥＴＲＩＣＲＥＶＥＲＳＥＤＦＬＯＷＩＮＪＥＣＴＩＯＮＭＥＴＨＯＤ￥ＪｉｎｇｆｕＬｉｕａｎｄＨｕｉｃｈａｎｇＭａ（ＲｅｓｅｔｒｃｈＣｅｎｔｅｒｆｏｒＥｃｏ－Ｅｎｖｉｒｏｎｍｅｎ...     

SYNTHESIS AND PROPERTIES OF HEPARINIZED POLYMETHACRYLIC ACID

ＳＹＮＴＨＥＳＩＳＡＮＤＰＲＯＰＥＲＴＩＥＳＯＦＨＥＰＡＲＩＮＩＺＥＤＰＯＬＹＭＥＴＨＡＣＲＹＬＩＣＡＣＩＤ￥ＦｅｎｇＰｉｎｚｈｅｎ；ＬｕＪｉａｎｔａｏ（ＤｅｐａｒｔｍｅｎｔｏｆＣｈｅｍｉｓｔｒｙ，ＧｒａｄｕａｔｅＳｃｈｏｏｌ，ＡｃａｄｅｍｉａＳｉｎ...     

甲硝唑的单扫示波极谱分析

本文系统地研究了甲硝唑在汞电极上的电极过程，证明了该电极过程为一含有吸附作用的不可逆的扩散控制过程，电子转移数为４，不可逆系数ａ＝０．７１４．建立了甲硝唑的单扫示波极谱测定法，在０．２０ｍｏｌ／Ｌ的ＮａＣｌ溶液中，于－０．７１Ｖ（ｖｓＳＣＥ）处的峰高与浓度［（０．０２─８０）ｍｇ／Ｌ］间存在良好的线性关系，检测限为２．４μｇ／Ｌ，ＲＳＤ＝２．０％（ｎ＝８），并考察了人体内常见微量物质对的影响。     

DETERMINATION OF CYCLOTRIME－THYLENETRINITRAMINE IN SERUM OF RABBIT BY RP－HPLC

ＤＥＴＥＲＭＩＮＡＴＩＯＮＯＦＣＹＣＬＯＴＲＩＭＥ－ＴＨＹＬＥＮＥＴＲＩＮＩＴＲＡＭＩＮＥＩＮＳＥＲＵＭＯＦＲＡＢＢＩＴＢＹＲＰ－ＨＰＬＣＣｈｅｎＹｏｎｇ；ＹｕａｎＺｈｕｏ－ｂｉｎ（ＧｒａｄｕａｔｅＳｃｈｏｏｌ，ＡｃａｄｅｍｉａＳｉｎｉｃａ．Ｂｅｉｊ...     

我国所有制结构的变动趋势预测与分析

我国所有制结构的变动趋势预测与分析刘国新，万君康（武汉工学院工商管理学院４３００７０）ＦｏｒｅｃａｓｔａｎｄＡｎａｌｙｓｉｓｏｆＴｒｅｎｄｏｎＣｈｉｎｅｓｅＯｗｎｅｒｓｈｉｐＳｔｒｕｃｔｕｒｅ…ＬｉｕＣｕｏｘｉｎＷａｎＪｕｎｋａｎｇＢａｓｅｄｏｎａｎ...     

烟雾吸入伤早期氧化与抗氧化动态平衡变化的实验研究

采用大鼠烟雾吸入伤模型，动态观察了伤后血浆、支气管肺泡灌洗液（ＢＡＬＦ）和肺组织中超氧化物歧化酶（ＳＯＤ）活性、丙二醛（ＭＤＡ）和共轭二烯（ＣＤ）含量的变化，辅以动脉血气分析、肺水量测定、ＢＡＬＦ中白细胞分类计数和病理检查．结果发现，伤后动物出现急性呼吸衰竭和严重肺水肿，肺内大量炎细胞聚集浸润；血浆、ＢＡＬＦ及肺组织中ＳＯＤ活性明显降低，ＣＤ和ＭＤＡ含量显著增加，ＭＤＡ／ＳＯＤ比值亦明显增高，且与ＢＡＬＦ中中性粒细胞数增加显著相关，提示氧化与抗氧化平衡失调可能在急性烟雾吸入性肺损伤的发生发展中起重要作用     

Normal delivery induced stress alters glutathione peroxidase and TNF-α in elderly primigravidas mononuclear cells

To probe the glutathione peroxidase (GPx) activity coupled to ROS and TNF-α levels in younger versus elderly primigravidas. Monocytes were isolated from blood of young and elderly primigravidas after 12 hrs, 24 hrs and 14 days postpartum. Age-matched healthy female donors served as controls. Thereafter, the above monocytes were subjected to evaluation of GPx activity, levels of pro-inflammatory cytokine TNF-α and reactivity/specificity towards anti-TNF-α antibody. Suppression in GPx activity with simultaneous enhancement of TNF-α levels in monocytes was observed after 12 hrs and 24 hrs of spontaneous labor pain-induced maternal delivery through normal vaginal route. Conversely, an enhancement of GPx activity and decrease in TNF-α levels were observed on day 14 of postpartum, thereby indicating reversal of the impaired neutralizing mechanisms. The antioxidant network combating ROS was stronger in younger primigravidas. Capability of TNF-α and ROS suppression was somewhat lesser in elderly primigravidas even 14 days of postpartum via normal vaginal route. The capability of GPx activity enhancement with simultaneous suppression in ROS and TNF-α, decreases in elderly primigravidas. This may act as one of the important parameters leading to a variety of complications encountered by elderly primigravidas.     

A biochemical study on variability of Superoxide dismutase,Catalase and Glutathione peroxidase in dry cleaners

Several chemicals which induce cytotoxicity are broadly classified into three types and they are organophosphate, organochlorine and carbomate compounds. Perchloroethylene has been clearly identified as a carcinogen in experimental animals and it’s a very common carcinogen used for dry clean purpose. Hence, in the present study, blood samples from dry cleaners were taken, Superoxide dismutase, Catalase and Glutathione peroxidase activity was estimated in blood samples of dry cleaners. There was a significant increase in the activity of Catalase, Glutathione Peroxidase, Superoxide Dismutase in dry cleaners of chronically exposed to perchloroethylene as compared to controls. The increased activities of antioxidant enzymes may be a compensatory regulation in response to increased oxidative stress induced by perchloroethylene.     

Age related changes in lipid peroxidation and antioxidants in elderly people

Advanced age is associated with an accumulation of free radical damage, which leads to physiological and clinical modifications. Age related changes resulting from free radical reactions include increasing levels of lipid peroxides, alterations in enzyme activities and greater osmotic fragility. The present study was conducted to estimate the level of lipid peroxidation product-Malondialdehyde and antioxidants Catalase and Glutathione in elderly people. An increase in lipid peroxidation and decrease in antioxidants was observed in normal elderly people. Highly significant increase in MDA and decrease in antioxidants was observed in elderly people when complicated with diabetes and hypertension. Supplementation of antioxidants may prevent further oxidative injury in elderly people.     

Detection of enzymes dehydrogenases and proteases inBrugia malayi filarial parasites

Lymphatic filariasis caused mainly by infection fromW. bancrofti andB. malayi remains a major cause of clinical morbidity in tropical and subtropical countries. Analysis ofB. malayi mf, infective larval and adult worm lysates for the activity of enzymes led to the demonstration of activities of three key enzymes of carbohydrate metabolism viz., Malate dehydrogenase (MDH), Malic enzyme (ME) and Glucose-6-phosphate dehydrogenase (G6PDH) in all the three stages of the parasite. The specific activity of all the three dehydrogenases was significantly high in mf lysate compared to their activity in lysates of the other two stages (P<0.001). Analysis by native polyacrylamide gel to their activity inlysates of the other two stages (P<0.001). Analysis by native polyacrylamide gel electrophoresis (PAGE) using 7.5% non-gradient gel showed the presence of two isoforms of each of the three enzymes (MDH, ME & G6PDH) in mf lysate, while only one form of each enzyme was present in L₃ larval and adult worm lysates. Further proteolytic enzyme activity was demonstrated both in microfilarial and infective larval lysates ofB. malayi. While both mf and L₃ larval lysates showed optimal protease activity at alkaline pH of 9.0, the mf lysate showed increased activity also at pH 3.0. The infective larval lysate was markedly inhibited by Tosylamide-L-Phenylalanine chloromethyl ketone (TPCK), a thiol protease inhibitor, while the protease activity in mf lysate was significantly inhibited by both TPCK and a serine protease inhibitor Phenyl Methyl Sulphonyl Flouride (PMSF). In sodium do-decyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), using gelatin copolymerized gel, the microfilarial lysate showed 3 protease molecules of 40 kDa, 180 kDa and 200 kDa and the L₃ larval lysate had 6 protease molecules of 18, 25, 37, 49, 70 and 200 kDa size.     

老年用户智慧养老服务需求及志愿服务意愿影响因素分析——以武汉市江汉区为例

通过对江汉区13 000多名老龄用户的调研,了解当地老年人的年龄情况、居住状况、经济状况及身体情况,分析其智慧养老服务需求及志愿服务意愿,并探究影响其需求和志愿服务意愿的因素,并依此描绘出目标用户特征,以辅助决策。数据分析结果显示：1）不同类别老年人其智慧养老服务需求和影响因素不同;2）不同年龄段的普通老人和5类老人,其智慧养老需求及志愿服务意愿的影响因素会因年龄段的不同而发生变化;3）智慧养老服务推广、实施的目标用户特征是：高龄、独居、身体较差的普通老人;高龄、独居、低收入、身体较差的5类老人;4）可发展成志愿者的目标用户特征为：独居/空巢普通老人;低龄、独居/空巢、低收入、身体较好的5类老人。     

Amplification of Antioxidant Activity of Haptoglobin(2-2)–Hemoglobin at Pathologic Temperature and Presence of Antibiotics

It is clear that Haptoglobin binds to Hemoglobin strongly and irreversibly. This binding, protects body tissues against heme-mediated oxidative tissue damages via peroxidase activity of Haptoglobin–Hemoglobin complex. Peroxidase activity of Haptoglobin(2-2)–Hemoglobin complex was determined via measurement of following increase in absorption of produced tetraguaiacol as the second substrate of Haptoglobin–Hemoglobin complex by UV–Vis spectrophotometer at 470 nm and 42°C. The results are showing that peroxidase activity of Haptoglobin(2-2)–Hemoglobin complex is modulated by homotropic effect of hydrogen peroxide as the allosteric substrate. On the other hand, antioxidant activity of Haptoglobin(2-2)–Hemoglobin is increased via heterotropic effect of two antibiotics (especially ampicillin) on the peroxidase activity of the complex. The condition of pathologic temperature along with the administration of ampicillin and/or coamoxiclav is in favor of amplification in antioxidant activity of Haptoglobin(2-2)–Hemoglobin and combating against free radicals in individuals with Hp2-2 phenotype. Therefore, oxidative stress effects have been diminished in the population with this phenotype.     

老年人口流动对福祉技术创新需求的分析

以全国流动人口抽样调查数据为数据源,细粒度的分析我国流动老年人面临的社会问题。研究发现,老年人口流动加重了异地医疗的需求,且对于流动老年人的疾病诊断和居家照料都提出了严峻挑战。针对以上流动老年人口面临问题,基于福祉技术创新解决问题的思路,并在政府、企业、高校等层面提出了策略性建议,促进福祉技术的快速发展,缓解老年人口流动带来的医疗和护理压力。     

Blood glutathione peroxidase and selenium in abortion

This study was carried out to estimate the levels of glutathione peroxidase and selenium in blood of abortion cases. Glutathione peroxidase and selenium were determined in 52 abortion cases (22 in 1^st trimester, 30 in second trimester), 45 normal pregnant cases and 25 nonpregnant control cases. The selenium concentration in whole blood and plasma in abortion cases was almost the same as in normal pregnant women but significantly low when compared with the control non-pregnant group. The glutathione levels was higher in abortion cases when compared with normal pregnant and non-pregnant control groups. Red cell and plasma glutathione peroxidase activities of women who had abortion were significantly lower compared with both non-pregnant control group and normal pregnancies.     

Calcineurin activity as an indicator of oxidative stress in normal islet cells and insulinoma cells

A comparative study was conducted to evaluate calcineurin activity in normal pancreatic beta cells and insulinoma cells in relation to their oxidative state. In comparison to normal islets, insulinoma cells had enhanced oxidative stress as evidenced by increased content of thiobarbituric acid reactive substances. In addition, diminished activity of calcineurin in insulinoma cells was concomitant with decreased content of reduced glutathione and glutathione peroxidase activity signifying diminished antioxidant status in these cells. Culturing insulinoma cells in presence of the calcineurin inhibitor cyclosporin A resulted in further decrease of calcineurin activity with restoration of glutathione peroxidase but without restoration of reduced glutathione levels. These results indicate that an estimate of oxidative stress in pancreatic islets and insulinoma cells can be obtained by assaying calcineurin activity.     

农村劳动力老龄化及其家庭结构差异对农地转出决策的影响

农地规模经营是实现现代农业的主要路径之一,探究劳动力老龄化与家庭农地转出决策,对促进小农户与现代农业的有机衔接有着重要意义。基于改进的家庭生命周期模型和中国家庭金融调查数据,从家庭层面实证检验了家庭劳动力老龄化对农地流转的影响及其家庭结构的异质性。结果发现：①中国现在还存有近1/3的农村家庭有老年人参与劳动,随着老龄化人口占比越来越高,以老年人为农业生产主体的小农户未来也可能长期存在;②劳动力老龄化对农地转出决策具有抑制作用。相比其他家庭类型,有老年劳动力的家庭转出农地的可能性降低了约5%;③不同家庭内部结构下,劳动力老龄化对家庭农地转出决策的抑制作用存在差异,抑制作用大小表现为负担期Ⅰ>赡养期Ⅰ>空巢期Ⅰ。本文认为,对于高龄的老年劳动力,需进一步建立健全农村社会保障制度,促进这部分农户通过以转代耕的方式把农地转出至农业生产效率更高的经营主体;对于低龄的老年劳动力,需配合较为完善的农业社会化服务以实现提升农业生产效率与农地养老的双重目标。