猪败血性链球菌病的诊治

猪败血性链球菌病是一种急性、败血性传染病．1994年5月至1997年3月在我区呈流行性，个别猪场呈爆发性，给我区的养猪业造成极大的经济损失．近几年来，我院收治败血性链球菌病猪906例，取得了一定的治疗经验．现将诊疗情况报告如下，以期对我区的养猪业起到一定的指导作用．1流行情况该病一年四季均可发生，大、小猪均能感染发病，以20～60kg的猪及产后一个月左右的母猪易发；饲养管理条件较差及喂食堂剩饭菜的猪群易发．同圈喂养的猪有亚～2头先发病，3～5日波及全群，若不及时采取措施，10～20天即蔓延整个猪场．据笔者对驻马店市小界牌…     

细小病毒MPV—Q株理化特性及其致病性的研究

细小病毒ＭＰＶ—Ｑ株是从患病雏番鸭体内分离的一种致病性病毒。电镜及理化特性检查表明该株病毒属细小病毒，圆形无囊膜，直径在２２—２４ｕｍ、对乙醚、胰蛋白酶、酸不敏感，对红细胞无凝集现象，对鸡、丽佳鸡、北京鸭、鹅无致病性。该病毒可引起雏番鸭发生以出血性肠炎为特征的急性败血性传染病。     

副猪嗜血杆菌的分离鉴定及药敏分析

采用常规细菌分离鉴定方法和分子生物学技术对四川某规模化猪场疑似副猪嗜血杆菌(Haemophilus parasuis,HPS)感染的病猪关节液、心包液、肺脏等病料进行了致病菌的分离与鉴定.经细菌分离培养特性、生化试验以及16S rDNA序列PCR扩增,确定分离菌株为HPS.动物致病性试验结果表明,分离菌株具有较强致病性.药敏试验表明,该HPS菌株对青霉素、链霉素、磺胺甲唑、林可霉素、苄唑西林等多数抗生素耐受,而对新霉素、头孢噻肟等较敏感.     

兔巴氏杆菌病诊疗报告

2005年7月间,我市立业六队饲养的家兔发生了一起由多杀性巴氏杆菌引的起的急性败血性传染病.该病的主要特征是败血和内脏器官出血,现报告如下.     

青霉素的发现简史

1928年,英国微生物学家弗荣明发现了青霉素。12年之后,英国病理学家弗洛里和生物化学家钱恩,终于分离得到了青霉素。青霉素的发现,开启了人类疾病治疗的新思路,成为第一个临床应用的抗生素,对人类的健康发展产生着深远的影响。     

非典型猪肺疫的诊断与防治

河南新乡地区某个体猪场于2000年2月发生了一起以发热跛行、咳嗽等症状和纤维素性肺炎，纤维素性心包炎、纤维素性肝炎等病理变化为特征的急性传染病。经综合诊断为非典型性猪肺疫，采用氨苄青霉素、青霉素等进行治疗，很快控制了疫情。 　　 1　流行情况 　　该猪场位于邻近居民生活区的低洼地带，存栏猪354头，其仔猪均为自繁自养。该场饲养的猪于2000年2月21日开始发病，至3月2日发病37头，发病率为10.5%。在此期间曾用青霉素、卡那霉素、安乃近、地塞米松类药物治疗，均无明显疗效。据调查，该场所用饲料为新乡大北农饮料厂生产的配合料，猪场用水为地下水，采用乳头式饮水器供水，发病猪包括怀孕母猪、后备母猪、育肥猪，其中4月龄至5月龄猪占总发病数的80.3%。 2　临床症状 　　病猪初期体温为39.3℃～40.5℃，食欲突然减退或拒食，精神沉郁，喜躺卧，严重病例两耳背侧出现少许出血斑点。病初便秘，后期腹泻，粪便恶臭，带有粘液。呼吸困难，初期表现为干咳，后期呈现湿咳。部分猪出现跛行，死前有神经症状，表现站立不稳，行走左右摇摆、倒地后四肢呈游泳状划动，病程在10～15d左右。     

溶氧电极在青霉素G发酵过程中的作用

青霉素G钾工业盐是氨苄青霉素及头孢类抗生素的原料药,青霉素发酵属单一纯茵种发酵,在发酵过程中,培养液中的溶解氧浓度对生长茵的代谢过程及最终产物的生物合成起着决定性的作用,本文详细阐述了梅特勒O2-Senso,溶氧电极在青霉素G发酵过程中的应用对青霉素发酵过程起着重要的指导意义.     

鉴别和分离微生物的实验设计

1利用选择培养基设计实验分离纯化微生物 在基本培养基中加入青霉素可分离纯化酵母菌、霉菌；在基本培养基中加入高浓度的食盐可分离纯化金黄色葡萄球菌。     

猪源肺炎克雷伯菌的分离鉴定及生物学分析

为了对河南省某猪场疑似细菌感染病例进行确诊,采集了病猪肠管中的样品并从中分离到1株革兰阴性短杆菌,对其进行了培养特性观察、生化试验、药敏试验以及16S r RNA基因序列分析.结果显示:分离菌的生化特性、培养特性基本符合肺炎克雷伯菌特征,16S r RNA基因序列与肺炎克雷伯菌的同源性均在98%以上,因此,将该分离菌株命名为Kpn;序列分析表明该分离菌株与猪源肺炎克雷伯菌NR114507的同源性最高,与大肠杆菌、普通变形杆菌、胸膜肺炎放线杆菌的同源性相对较低;分离株具有较强的致病性和多重耐药性,对美洛西林、哌拉西林、强力霉素、美满霉素、头孢哌酮敏感,但对氨苄西林、先锋霉素IV、先锋霉素V、先锋霉素VI、卡那霉素、新霉素等多种药物耐药.研究为猪肺炎克雷伯菌的分离鉴定及治疗提供了参考.     

副猪嗜血杆菌病病原的分离培养与PCR鉴定

根据某猪场患病猪的临床症状和剖检变化,初步诊断为副猪嗜血杆菌引起,为确诊该病,随即取患病猪组织进行实验室检查.结果从患病猪的胸水、腹水中分离到2株革兰氏阴性细小杆菌,其生化试验为:接触酶阳性,氧化酶、吲哚和脲酶阴性;发酵果糖、麦芽糖、葡萄糖和蔗糖;不分解阿拉伯糖、鼠李糖、木糖、甘露醇、乳糖.生长需要V因子.用副猪嗜血杆菌的16S rRNA的特异性PCR引物,通过PCR技术分别从该分离菌株扩增出828 bp的特异基因片段,表明该分离菌株为副猪嗜血杆菌.为疫苗的研制提供了试验依据.     

鸭传染性浆膜炎的诊断及防治试验

鸭传染性浆膜炎又称鸭疫里氏杆菌病,是一种主要侵害幼禽的急性或慢性接触性细菌性传染病。本文根据病鸭的发病情况、临床症状、剖检变化及实验室诊断确诊为鸭传染性浆膜炎。并进行了药敏试验,得到了对本分离菌株敏感的药物,用敏感药物配合中药新菌灵对患病鸭进行了治疗,治愈率达93%.     

Case Report:White-spot disease of Chinese soft-shelled turtles (Trionyx sinens) caused by Paecilomyces lilacinus

Chinese soft-shelled turtles (Trionyx sinens) in culture farms using an artificial warming system in Zhejiang, China,often show typical signs of white-spot disease such as white spots on their bodies, skin lesions, anorexia and eventually death. The sick turtles were mostly 5～80 g in weight. A suspected fungai pathogen was isolated from the sick turtles and verified as Pae-cilomyces lilacinus by sequence analysis of the internal transcribed spacer (ITS) of its ribosomal DNA (rDNA). Detailed mor-phological examinations were also conducted to confirm the white-spot disease.     

White-spot disease of Chinese soft-shelled turtles (Trionyx sinens) caused by Paecilomyces lilacinus

Chinese soft-shelled turtles (Trionyx sinens) in culture farms using an artificial warming system in Zhejiang, China, often show typical signs of white-spot disease such as white spots on their bodies, skin lesions, anorexia and eventually death. The sick turtles were mostly 5~80 g in weight. A suspected fungal pathogen was isolated from the sick turtles and verified as Paecilomyces lilacinus by sequence analysis of the internal transcribed spacer (ITS) of its ribosomal DNA (rDNA). Detailed morphological examinations were also conducted to confirm the white-spot disease.     

A molecular biological study on identification of common septicemia bacteria using 16s–23s rRNA gene spacer regions

In the search for a rapid and reliable method for identification of bacteria in blood and cerebrospinal fluid , we developed a unified set of primers and used them under polymerase chain reaction(PCR) to amplify the spacer regions between the 16s and 23s genes in the prokaryotic rRNA genetic loci . Spacer regions within these loci showed a significant level of length and sequence polymorphism across most of the species lines. A generic pair of priming sequences was selected from highly conserved sequences in the 16s and 23s genes occurring adjacent to these polymorphic regions. This single set of primers and reaction conditions were used for the amplification of the 16s-23s spacer regions for 61 strains of standard bacteria and corresponding clinical isolates belonging to 20 genera and 27 species, including Listeria, Staphylococcus and Salmonella species, et al. When the spacer amplification products were resolved by electrophoresis, the resulting patterns could be used to distinguish most of the bacteria species within the test group, and the amplification products of the clinical isolates clustered at the standard species level. Some species presenting similar pattern were further analyzed by HinfI or AluI digestion or DNA clone and sequences analysis in order to establish the specific 16s-23s rRNA gene spacer regions map. Analysis of 42 blood specimens from septicemic neonates and 6 CSF specimens from suspected purulent meningitis patients by bacterial culture and PCR-RFLP(Restriction Fregament Length Polymorphism) showed that 15 specimens of blood culture were positive(35.7%) in the 42 septicemic neonates; 27 specimens were positive(64.2%) by PCR, and that the positive rate by PCR was significantly higher than that by blood culture(P<0.01). Among the 6 CSF specimens, one specimen found positive by blood culture was also positive by PCR, two found negative by blood culture showed positive by PCR; all three were S.epidermidis according to the DNA map. One C.neoformans found positive by blood culture showed negative by PCR. The remaining two specimens were both negative by PCR and blood culture. These results indicated that the method of detecting bacterial 16s-23s rRNA spacer regions using PCR and RFLP techniques was rapid, sensitive and specific in the detection of bacterial infections; and so, has very important application in the clinical diagnosis of sepsis in neonates.     

鸡场禽流感的诊断

本实验对某鸡场鸡群中出现的一种以呼吸道症状和产蛋下降为主要特征的疫情进行了临床症状和流行病学调查,对病死鸡进行了解剖检查并从病鸡病料中分离到一种病毒,通过血凝(HA)和血凝抑制(HI)试验、病毒中和试验(VNT)和琼脂免疫扩散试验(AGP)等方法对该病毒进行了签字。结果表明该次疫情为A型流感病毒感染。     

Acute phase reactants, challenge in the near future of animal production and veterinary medicine

INTRODUCTION The systemic acute phase reaction known to occur on infection, inflammation, trauma, burns, ma- lignancies and tissue damage in general, has been studied by scientists from various disciplines. In the last decade, emphasis has been laid on application of blood tests for acute phase reactants to monitor ani-mal health in general, as well as for human patients suffering from specified classes of diseases (Gruys et al., 2005). However, basic mechanistic patterns as- sociated wit…     

论李贺诗歌的生命意识

中唐诗人李贺多病而敏感,他对生与死的认识和体验与同时代的诗人相比,尤为深刻,这就是他诗歌里所表现出的强烈的生命意识。同时,在儒家修齐治平的主流文化背景之下,李贺的生命意识还包含人生价值和意义的追求,这个没落的王孙对唐王朝的兴衰有着道义上的责任,也有重振家道、光耀门楣的责任。李贺的痛苦正是对生与死的敏感体认和人生价值和意义难以实现的深刻痛苦。把握李贺诗歌中所表现出的生命意识,对于理解他的诗歌具有重要意义。     

急性脑损伤患者血清LDH的检测研究

目的:探讨急性脑损伤患者血清乳酸脱氢酶变化的临床意义.方法:AbbottAeroset(2000)全自动生化分析仪测定乳酸脱氢酶(LDH).结果:2组病人在急性脑损伤后LDH明显高于对照组(P＜0.01),组间比较差异不明显(P＞0.05).结论:测定血清乳酸脱氢酶可在一定程度上反应脑损伤的程度,对预后和治疗有一定的指导意义.     

Physician‐patient communication: Mutual role expectations

This study examined physician‐patient communication as it relates to expectations that patients and physicians hold for themselves and each other. Dimensions of mutual role expectation were determined to be physician as humanistic practitioner, physician authority, patient in the sick role, and patient as consumer. The study tested five hypotheses. Results showed patients had significantly higher expectations for patients in the sick role than had physicians. Other role expectations varied significantly by age within physician and patient groups. The implications of these differences for physician‐patient communication are discussed.     

A virtual learning environment for the continuation of education and its relationship with the mental well-being of chronically ill adolescents

Research has shown that the continuation of education by chronically ill adolescents is an important way to avoid social isolation, psychosocial problems and the accumulation of learning difficulties. In this light, virtual learning environments (VLEs), which connect sick adolescents to their schools, play an important role in ensuring that the sick adolescents continue their education, and in contributing to their mental well-being. This study investigated the extent to which a VLE supports the continuation of education and school interactions among sick adolescents and how it is related to their mental well-being. The results show that the VLE had a positive impact on supporting the continuation of education and school interactions among chronically sick adolescents and was considered very helpful to their mental well-being.