溃疡病胃窦粘膜β——内啡肽样免疫反应细胞的体视学定量研究

应用真彩色图象分析仪,对15例胃溃疡(GU)、十二指肠溃疡(DU)和浅表性胃炎作对照组(Con)患者,非病变区胃窦粘膜的151个β—内啡肽细胞(β—EP 细胞)形态计量学参数及45个测试单位面积(5000μm~2)的细胞数进行检测,收集13项参数行统计学 t 检验。呈高度显著性差异的参数分别有三组间的周长(C)、面积(A)、长径(LD)、短径(SD)和平均体积(V);DU 与 Con,GU 与 Con 组的比表面(δ),平均截距(τ)、平均面积(?)和平均直径((?)c);DU 与 GU、或与 Con 组的细胞均数(N)。三组中各参数的显著性差异率 C、A、LD、SD、(?)达100%,δ、τ、(?)、(?)c、N 为60%以上。本文首次应用真彩色图象分析仪,建立了 DU、GU 和 Con 组非病变区胃窦粘膜幽门腺β—内啡肽细胞的二维和三维体视学、形态计量学参数及细胞均数数据,并为其参与内分泌调节提供了客观依据。     

对人胃癌胃窦粘膜幽门腺生长抑素样免疫反应细胞的研究

本文用免疫组织化学和显微图像分析,分别对胃癌及对照组肉眼下非病变区胃窦粘肿幽门腺生长抑素样免疫反应细胞(D细胞)进行了研究。结果表明:胃癌和对照组D细胞主要分布在胃窦粘膜中下部,胃癌 D细胞减少,其数密度与对照组相比有高度显著性差异(P<0.01)。胃癌组形状因子增大,与对照组相比有显著性差异(P<0.05)。本文为探讨胃癌时胃窦粘膜非病变区幽门腺D细胞的内分泌调节提供了形态学信(?)。     

消化性溃疡病患者胃窦粘膜幽门腺5-HT细胞和G细胞免疫组织化学研究

胃溃疡(Gu)、十二指肠溃疡(Du)和对照组(Con)各5例,取肉眼下非病变区胃窦粘膜,用免疫组织化学PAP双重染色法,在同一张切片上显示5-HT细胞和G细胞。结果5-HT细胞多散在于粘膜的中下1/3,呈棕褐色,形状多样,常见有突起的细胞。G细胞呈带状分布于粘膜的中1/3,大部分G细胞为蓝色,个别细胞为蓝黄混合色。它们多为圆形或锥体形。有时可见5-HT细胞和G细胞相毗邻。5-HT细胞数和G细胞数在Gu组分别为:4.17±1.21、7.87±3.13;Du组:5.45±1.96、9.21±5.72;Con组:3.21±1.64、6.19±1.72。5-HT细胞数和G细胞数在Gu组和Du组都增加。且都以Du组为最多,经相关分析二者呈正相关关系。Gu和Du组的5-HT细胞数和G细胞数与对照组相比均有高度显著性差异。Du组的5-HT细胞数和G细胞数与Gu组相比较,亦有高度显著性差异。提示5-HT细胞和G细胞可能参与了消化性溃疡病的调节,特别与Du的关系更为密切。5-HT细胞和G细胞之间的关系有待于进一步研究。     

消化性溃疡病患者胃窦粘膜幽门腺5-HT细胞和G细胞免疫组织化学研究

胃溃疡(Gu)、十二指肠溃疡(Du)和对照组(Con)各5例,取肉眼下非病变区胃窦粘膜,用免疫组织化学PAP双重染色法,在同一张切片上显示5-HT细胞和G细胞。结果5-HT细胞多散在于粘膜的中下1/3,呈棕褐色,形状多样,常见有突起的细胞 G细胞呈带状分布于粘膜的中1/3,大部分G细胞为蓝色,个别细胞为蓝黄混合色。它们多为圆形成锥体形、有时可见5-HT细胞和G细胞相毗邻,5-HT细胞数和G细胞数在Gu组分别为:4 17±1.21、7.87±3 13;Du组:5 45±1 96、9 21±5 72;Con组:3.21±1.64、6 19±1.72。5-HT细胞数和G细胞数在Gu组和Du组都增加,且都以Du组为最多,经相关分析二者呈正相关关系,Gu和Du组的5-HT细胞数和G细胞数与对照组相比均有高度显著性差异 Du组的5-HT细胞数和G细胞数与Gu组相比较,亦有高度显著性差异。提示5-HT细胞和G细胞可能参与了消化性溃疡病的调节,特别与Du的关系更为密切,5-HT细胞和G细胞之间的关系有待干进一步研究。     

人体胃癌期间胃窦粘膜幽门腺5—TH样免疫反应细胞研究

本文用免疫组织化学PAP法染色,观察胃癌组与对照组之间胃窦粘膜幽门腺非病变区5—HT样免疫反应细胞的变化。结果:5—HT样免疫反应细胞在癌症组明显增加,与对照组相比有显著性差异,提示5—HT样免疫反应细胞可能参与胃癌期间的调制。     

人胎儿胃窦粘膜胃泌素细胞的免疫组织化学研究

用免疫组织化学PAP法对45例2月～足月新鲜胎儿及出生后2天婴儿的胃窦胃泌紊细胞(G细胞)进行了定性和定量的研究。G细胞于胎龄3个月的胎儿胃窦粘膜内开始出现,数量极少。以后随胎龄的增长而递增。G细胞形态多样,有的细胞有突起,并可借突起与其他腺细胞相接触。细胞的顶端或突起朝向或达到管腔。本观察结果提示G细胞除具有内分泌和旁细胞分泌的形态学证据。     

小鼠实验性胃溃疡自愈期间注射秋水仙碱观察G细胞的变化

用雄性昆明种小鼠45只,分为实验性溃疡组和对照组,后者又分为盐水组和空白组。在实验性胃溃疡术后3、6、9和20d,腹腔注射秋水仙碱3小时后,取胃窦组织。用免疫组织化学和苏木精染色。观察胃窦粘膜G细胞的变化。正常胃窦粘膜幽门腺分裂指数5.93±1.23;G细胞百分率2.76±0.45;G细胞分裂指数Ⅰ0.85±0.18;G细胞分裂指数Ⅱ0.023±0.01。幽门腺细胞分裂指数、G细胞百分率及其分裂指数Ⅱ,在术后6、9和20d与盐水组相比,呈高度显著性差异;G细胞分裂指数Ⅰ,术后9d与空白组、术后20d与盐水组相比,均呈显著性差异。本文讨论了以上变化的意义。     

胃窦粘膜幽门腺内分泌细胞5-HT和胃泌素的共存

目的观察胃窦粘膜幽门腺内分泌细胞中5-HT和胃泌素共存与否.方法应用已树脂封片的成人胃窦粘膜5-HT细胞再行双重PAP法显示G细胞的方法,在同一张切片上进行5-HT和胃泌素的双重染色.结果5-HT细胞多散在于粘膜的中下1/3,呈棕褐色,形态多样,常见有突起的细胞.G细胞呈带状分布于粘膜的中1/3,大部分G细胞为蓝色,呈圆形或锥体形.有时可见5-HT细胞和G细胞相比邻,有趣的是可见少量细胞为蓝黄混合色.结论在胃窦粘膜幽门腺内分泌细胞中,可见5-HT与胃泌素的共存.     

大鼠实验性胃溃疡期间胃窦粘膜胃泌素细胞的免疫组织化学和放射自显影研究(摘要)

成年雄性Wistar大鼠16只,分为实验溃疡组和对照组,后者又分为盐水组和空白组。在手术后4、6、10和21天取胃窦粘膜,经离体~3H—TdR掺入,用免疫组织化学和放射自显影方法观察胃泌素细胞(G细胞)的变化。正常大鼠胃窦粘膜G细胞标记指数为1.59±0.78,细胞百分率为4.27±0.27。溃疡组G细胞标记指数在手术后4天比对照组降低,10天的比对照组增高;G细胞百分率在     

人胃粘膜中D,5-HT和G细胞与年龄相关性研究

为了探讨年龄对胃内分泌细胞数目的影响,本文取胃镜或胃切除标本67例,按年龄分青年组(22～34岁)和老年组(67～78岁)。石蜡切片,厚度5μm,用免疫组织化学双重染色方法,观察了胃体和胃窦部生长抑素细胞(D)、5-羟色胺(5-HT)和胃泌素(G)免疫阳性细胞,经光镜下观察和细胞计数表明青年组三种细胞明显多于老年组,在胃窦部D、G细胞数青年组显著多于老年组(P<0.05),5-HT细胞则两组无明显差异(P>0.05),在胃体部,青年组三种细胞均多于老年组(P<0.05),文中对三种细胞的功能和检测它们的意义进行了讨论。     

月鳢血液的研究及其与性别的关系

对月鳢血细胞的显微结构、数量、大小,核的大小,白细胞的分类计数及血红蛋白含量等方面进行了观察、测定,并进行了雌雄两性比较。结果表明,月鳢的血红蛋白量为（8.06±1.78）g/100ml;红细胞数量值为222.52±57.53（10^4/mm^3）;白细胞数量值为2.25±0.51（10^4/mm^3）;白细胞分类计数中淋巴细胞占（68.78±4.68）%,单核细胞占（8.42±1.78）%,血栓细胞占（7.59±5.54）%,嗜中性粒细胞占（15.53±5.54）%;红细胞大小（长径×短径）为（12.64±0.48）μm×（7.44±0.30）μm;红细胞核大小（长径×短径）为（6.02±0.29）μm×（1.89±0.14）μm。性别对血细胞的形态结构及血液指标无显著影响。     

长短桩、变径桩桩间相互作用系数数值研究

采用三维数值方法,建立长短桩、变径桩双桩模型,通过大量的数值计算,分析桩顶荷载水平、桩距径比、不同桩长、桩端土模量比及不同桩径对相互作用系数的影响。分析表明,在线弹性变形阶段,桩-桩相互作用系数近似为恒定值,与桩顶荷载水平无关,进入塑性变形之后,则逐渐减小直至极限状态时趋于0;长短桩桩长比及桩端土模量比对短桩-长桩相互作用系数影响大,而对长桩-短桩相互作用系数影响小;变径桩相互作用系数仅与受荷桩桩径有关;并提出了针对长短桩、变径桩的简化计算公式,为实际工程提供参考。     

麝毛被扫描电镜观察和机能意义探讨

麝毛被由长针毛和短针毛组成,无绒毛。长针毛的髓部和皮部由致密纵横膈分隔成气室,气室连续贯穿毛干,毛小皮呈菱形。短针毛髓部为气室,皮部为致密角质物,毛小皮呈扁平形。毛干气室发达充盈体表温度,毛被层形成体表保温层,具保温御寒功能。     

大鼠实验性胃溃疡期间大脑皮层SS免疫组织化学和原位杂交组织化学研究

目的 观察大鼠实验性胃溃疡期间大脑皮层生长抑素免疫反应(SS-ir)和SSmRNA神经元的变化。方法 免疫细化PAP法;地高辛标记反意SScRNA探针mRNA原位杂交组化法。结果 正常大鼠SS-ir神经元广泛分布于大脑皮层。溃疡术后1d,大脑皮层纹状皮质区各层SS-ir神经元减少,但可见大量串珠样SS强阳性纤维,溃疡组SS-ir神经元数与盐水组相比无差异;术后4d,溃疡组大脑皮层纹状皮质区各层SS-ir神经元密集、数量明显增多,与盐水组、正常组相比均有差异(P<0.05,P<0.01或P<0.001)。术后10和23d,以上核区SS-ir神经元数仍高于盐水组和正常组(P<0.01,P<0.001)。术后4d,溃疡组大脑皮层SSmRNA神经元数量明显多于盐水组(P<0.001)。结论 大鼠实验性胃溃疡时期,大脑皮层SS-ir和SSmRNA神经元可能参与了胃溃疡自愈过程的调节。     

毛冻绿幼叶的显微形态研究

目的:探究鼠李科鼠李属植物毛冻绿(Rhamnaceae utilis Decne.var.hypochrysa(schneid.)Rehd.)春季初萌发幼叶的显微鉴别特征。方法:徒手切片观察幼叶纵切结构和叶片上下表皮表面观,粉末显微辨识毛冻绿幼叶特征结构与形态。结果:毛冻绿非腺毛可分为长、短两种类型,非腺毛毛囊处的纵皱纹理明显,且放射性地向毛囊基部表面扩散。毛囊处于叶表皮细胞整体水平面以下且顶部细胞呈细长柱形,侧面观呈鞭状的非腺毛为长非腺毛;毛囊处于叶表皮细胞整体水平面上且毛囊与叶表皮细胞连接部分呈水滴状,短非腺毛侧面观近三角形;短非腺毛的分布常与叶肉细胞中的方晶伴生。结论:利用毛冻绿非腺毛的毛囊与叶表皮细胞连接的位置关系,及非腺毛长度的形态差异,可作为毛冻绿幼叶的显微鉴别提供依据。长短非腺毛在不同物候环境变化下,形态会有过渡性变化。长非腺毛可能与保障蒸腾与呼吸作用有关,短非腺毛可能与保护叶肉组织有关。     

THE CHANGING FACE OF THE UNIVERSITY AND THE INTERNATIONALIZATION OF TEACHING AND RESEARCH

As everyone knows, the aims and the goals of university life are quite frequently contradictory. This characteristic is no less true of the internationalization of higher education by means of various forms of international co‐operation: faculty and student exchanges and the like. Often the universities of given nations will engage in such ventures so that they and their backers, specific national enterprises, can gain a competitive edge, one over the other, in areas that produce short‐term wealth for specific interests rather than long‐term solutions to such vital world problems as the destruction of the ozone layer, air and water pollution, and the depletion of vital natural resources. Academics who are true internationalists will see to it that solutions for these and for other “real” problems of mankind are given first priority.     

LIFELONG EDUCATION AND THE UNIVERSITIES OF THE UNITED KINGDOM

The higher education system of the United Kingdom, of which the universities are only part, is changing rapidly. Although broad social conditions explain why universities should be centres of lifelong learning and specific institutional needs are in fact pushing them in this direction, changing demography is only one explanation among many. The kinds of programmes and related policies concerning students, teaching and curricula which are seen as relevant to continuing or lifelong education are widening and diversifying as the political and funding environment of higher education also changes. Research on continuing education and that generated by it are also being perceived differently. The new decade of the 1990's seems likely to witness significant changes in what is identified as continuing education as present trends in favour of integration or mainstreaming accelerate and new arrangements are made for more accessible and flexible forms of study both for degrees and for short courses. This process will be a part of a wider and more fundamental change as higher education moves somewhat towards a mass from an élite model and as the concept of a university and of a university education changes in step behind these changes in practice. The possibility exists that more fundamental changes in the initial undergraduate curriculum will also make universities more effective centres in terms of preparing their young students for lifelong learning.     

PSYCHOLOGICAL SKILLS PROFILES OF ATHLETES: THE EFFECTS OF FEEDBACK

In an interrupted time series design with switching replications, three groups of club golfers received feedback on their psychological and psychomotor skills and level of involvement in golf as measured by the scales of the Golf Performance Survey. Repeated measures on these skills and performance levels in competition were obtained in the weeks before and after the feedback sessions to evaluate both short and long term effects. Multivariate analyses of the data revealed some improvement in the psychological and psychomotor skills of the participants, but no significant effects on performance. Although the profiling of strengths and weaknesses is often regarded as an important component of performance enhancement interventions, the results of this study suggest that subsequent training in those skills is necessary if the interventions are to be effective with athletes.