家蚕核型多角体病毒p35基因的核苷酸序列分析

对家蚕核型多角体病毒苏州株（ＢｍＮＰＶｓｕ）ｐ３５基因的序列分析表明：ＢｍＮＰＶｓｕ ｐ３５编码序列为８９７ｍｔ,编码２９８ａａ。同源性分析表明：ＢｍＮＰＶｓｕ ｐ３５与ＢｍＮＰＶＴ３、ＡｃＮＰＶ 、ＳＩＮＰＶ在核苷酸水平上同源性分别为９９．５％、９５．１％、８９．３％,在氨基酸水平上的 性分别为９８．７％、８９．４％、７６．４％,显示了杆状病毒ｐ３５基因在进化上的保守性。ＢｍＮＰＶＴ３中位的Ｎ１     

鲑鱼生长激素cDNA5'端和3'端的修饰与在原核细胞中表达的初步研究

为了研究鱼生长激素基因在大肠杆菌中的表达,采用聚合酶链反应（ＰＣＲ）技术对鲑鱼生长激素ｃＤＮＡ的５端和３端进行定向修饰．将修饰后的三种基因分别克隆到大肠杆菌表达质粒ｐＢＶ２２０,构建成重组鲑鱼生长激素表达质粒ｐＢＶＧＨ１１,ｐＢＶＧＨ１８和ｐＢＶＧＨ２４,转化大肠杆菌ＤＨ５α进行诱导表达．结果表明：核糖核蛋白体结合位点（ＳＤ）与起始密码子ＡＴＧ之间的距离对生长激素的表达水平影响极大．鲑鱼生长激素基因的终止密码子ＴＡＧ不能有效终止该基因在大肠杆菌中翻译的进行,造成部分通读．加入大肠杆菌强终止子ＴＡＡ可避免通读和产生超长蛋白,提高表达产量     

A RELATION AMONG ASSOCIATIVE ALGEBRAS,BIALGEBRAS AND SEMIGROUP ALGEBRAS

ＡＲＥＬＡＴＩＯＮＡＭＯＮＧＡＳＳＯＣＩＡＴＩＶＥＡＬＧＥＢＲＡＳ，ＢＩＡＬＧＥＢＲＡＳＡＮＤＳＥＭＩＧＲＯＵＰＡＬＧＥＢＲＡＳＬｉＦａｎｇ（李方）（ＤｅｐａｒｔｎｉｅｎｔｏｆＭａｔｈｅｎｉａｔｉｃｓａｎｄＭｅｃｈａｎｉｃｓ）ＡＲＥＬＡＴＩＯＮＡＭＯ...     

SDS—PAGE区别鉴定IBDV和ARV

鸡传染性法氏囊炎病毒（ＩｎｆｅｃｔｉｏｎｓＢｕｒｓａｌＤｉｓｅａｓｅＶｉｒｎｓ，ＩＢＤＶ）和禽呼肠孤病毒（ＡｖｉａｎＲｅｏｖｉ－ｒｕｓ，ＡＲＶ）的许多生物学特性和理化特性十分相似［１－４］，尤其在形态结构和理化特性上极为相似［５］，本文用ＳＤＳ—ＰＡＧＥ区别鉴定ＩＢＤＶ和ＡＲＶ是一种简便、快速、可靠的方法。     

INFLUENCE MEASURE OF PERTURBATION AND VARIABLES IN REGRESSION MODELS

ＩＮＦＬＵＥＮＣＥＭＥＡＳＵＲＥＯＦＰＥＲＴＵＲＢＡＴＩＯＮＡＮＤＶＡＲＩＡＢＬＥＳＩＮＲＥＧＲＥＳＳＩＯＮＭＯＤＥＬＳＨｕＹｕｅｑｉｒｉｇ（胡跃清）（ＤｅｐａｒｔｍｅｎｔｏｆＭａｔｈｅｍａｔｉｃｓａｎｄＭｅｃｈａｎｉｃｓ）ＩＮＦＬＵＥＮＣＥＭＥＡＳ...     

大豆β-1,3-葡聚糖酶基因转化烟草及抗病性的研究

构建了大豆β－１，３－葡聚糖酶（β－１，３－ｇｌｕｃａｎａｓｅ）基因的植物表达载体ｐＢＩ１２１－ｇｌｕ，并通过直接转化方法将其导入根癌农杆菌（Ａ．ｔｕｍｅｆａｃｉｅｎｓ）ＬＢＡ４４０４受体菌中，构建了用于植物遗传转化的工程菌株ＬＢＡ４４０４（ｐＢＩ１２１－ｇｌｕ），并以烟草为转化对象进行了遗传转儿，获得了大量再生的转基因烟草．ＰＣＲ，ＰＣＲ－Ｓｏｕｔｈｅｍ以及Ｓｏｕｔｈｅｍ杂交检测结果表明目的基因已整合到烟草基因组中．转基因植株苗期抗立枯病实验表明，部分转化β－１，３－葡聚糖酶基因的工程烟草对立枯丝核菌（Ｒｈｉｚｏｃｔｏｎｉａ　ｓｏｌａｎｉ．）表明出不同程度的抗性提高．     

PARAMETER IDENTIFICATION OF LINEAR SYSTEM VIA GENERAL HYBRID ORTHOGONAL FUNCTIONS

ＰＡＲＡＭＥＴＥＲＩＤＥＮＴＩＦＩＣＡＴＩＯＮＯＦＬＩＮＥＡＲＳＹＳＴＥＭＳＶＩＡＧＥＮＥＲＡＬＨＹＢＲＩＤＯＲＴＨＯＧＯＮＡＬＦＵＮＣＴＩＯＮＳＹｉｎＪｉａｈｏｎｇ（尹家洪）（ＤｅｐａｒｔｎｉｅｎｔｏｆＭａｔｈｅｎｉａｔｉｃｓａｎｄＭｅｃｈａｎｉｃ...     

AN INTERACTIVE METHOD FOR THE MULTIOBJECTIVE DECISION BASED ON THE WEIGHTED TCHEBYCHEFF NORM

ＡＮＩＮＴＥＲＡＣＴＩＶＥＭＥＴＨＯＤＦＯＲＴＨＥＭＵＬＴＩＯＢＪＥＣＴＩＶＥＤＥＣＩＳＩＯＮＢＡＳＥＤＯＮＴＨＥＷＥＩＧＨＴＥＤＴＣＨＥＢＹＣＨＥＦＦＮＯＲＭＳｈｅｎＨｏｕｃａｉ（沈厚才）；ＺｈｏｎｇＷｅｉｊｕｎ（仲伟俊）；ＸｕＮａｎｒｏｎｇ（徐南...     

BIFURCATION ANALYSIS OF THESTEADY-STATE MODEL FOR A MULTIJUNCTION DEVICE

ＡＢＩＦＵＲＣＡＴＩＯＮＡＮＡＬＹＳＩＳＯＦＴＨＥＳＴＥＡＤＹ－ＳＴＡＴＥＭＯＤＥＬＦＯＲＡＭＵＬＴＩＪＵＮＣＴＩＯＮＤＥＶＩＣＥＣａｏＪｕｎｃｈｅｎｇ（曹俊诚）；ＷｅｉＴｏｎｇｌｉ（魏同立）（ＭｉｃｒｏｅｌｅｃｔｒｏｎｉｃｓＣｅｎｔｅｒ）ＷａｎｇＹ...     

IMPLEMENTATION OF DISCONTINUOUS COEFFICIENT INVERSION FOR A CLASS OF HYPERBOLIC EQUATION

ＩＭＰＬＥＭＥＮＴＡＴＩＯＮＯＦＤＩＳＣＯＮＴＩＮＵＯＵＳＣＯＥＦＦＩＣＩＥＮＴＩＮＶＥＲＳＩＯＮＦＯＲＡＣＬＡＳＳＯＦＨＹＰＥＲＢＯＬＩＣＥＱＵＡＴＩＯＮＬｉｕＪｉｊｕｎ（刘继军）（ＤｅｐａｒｔｍｅｎｔｏｆＭａｔｈｅｍａｔｉｃｓａｎｄＭｅｃｈａｎｉ...     

浅析制约运动技能获得的若干问题

获得运动技术牵涉着诸多因素,其中教练员与运动员之间的语言交流,兼优化互补内在自身过程是有效掌握技能的关键。     

本原、本体、本质、辩证与有无——论老子“道”论意涵的五个维度

"道"论作为老子哲学思想的核心内容,它的出现标志着中国传统哲学理论发展到了一个全新的水平。由本原、本体、本质、辩证与有无五个维度出发,对"道"论的意涵进行阐释,是一次全新的尝试。这一尝试对于我们从宇宙本原、根本存在、自然本质、辩证法思想、"有"和"无"的义理来把握"道"有着极为重要的意义。同时,也对"道"论的传统价值和现实意义进行了说明。     

明末宋玉资料的辑佚、整理、编辑与其对文学宋玉的传播

明末,有两部辑有宋玉资料的著述,即张燮《七十二家集.宋大夫集》和周圣楷《楚宝.宋玉》。我们通过对两书宋玉资料辑佚、整理、编辑的分析,认为张燮的宋玉资料辑佚与周圣楷的宋玉资料编辑具有互补作用,两者参看可以比较全面地了解宋玉,比较正确地认知宋玉,尤其是能够引导读者从文学的视角审视宋玉;张燮和周圣楷的宋玉资料辑佚、整理、编辑对于文学宋玉的传播有着极其重要的意义。     

Simultaneous determination of fluoride, chloride, sulfate, phosphate,monofluorophosphate, glycerophosphate, sorbate, and saccharin in gargles by ion chromatography

Simple, reliable and sensitive analytical methods to determine anticariogenic agents, preservatives, and artificial sweeteners contained in commercial gargles are necessary for evaluating their effectiveness, safety, and quality. An ion chromatography (IC) method has been described to analyze simultaneously eight anions including fluoride, chloride, sulfate, phosphate,monofluorophosphate, glycerophosphate (anticariogenic agents), sorbate (a preservative), and saccharin (an artificial sweetener)in gargles. In this IC system, we applied a mobile phased gradient elution with KOH, separation by IonPac AS18 columns, and suppressed conductivity detection. Optimized analytical conditions were further evaluated for accuracy. The relative standard deviations (RSDs) of the inter-day's retention time and peak area of all species were less than 0.938% and 8.731%, respectively,while RSDs of 5-day retention time and peak area were less than 1.265% and 8.934%, respectively. The correlation coefficients for targeted analytes ranged from 0.999 7 to 1.000 0. The spiked recoveries for the anions were 90%～102.5%. We concluded that the method can be applied for comprehensive evaluation of commercial gargles.     

略论教育的定位、导向、谋策、履责

教育应积极主动适应经济的跨越式发展战略对人才培养的需要，务必解决好定位、导向、谋策、履责。     

Science Letters： Simultaneous determination of fluoride, chloride, sulfate, phosphate, monofluorophosphate, glycerophosphate, sorbate, and saccharin in gargles by ion chromatography

Simple, reliable and sensitive analytical methods to determine anticariogenic agents, preservatives, and artificial sweeteners contained in commercial gargles are necessary for evaluating their effectiveness, safety, and quality. An ion chromatography （IC） method has been described to analyze simultaneously eight anions including fluoride, chloride, sulfate, phosphate, monofluorophosphate, glycerophosphate （anticariogenic agents）, sorbate （a preservative）, and saccharin （an artificial sweetener） in gargles. In this IC system, we applied a mobile phased gradient elution with KOH, separation by IonPac AS18 columns, and suppressed conductivity detection. Optimized analytical conditions were further evaluated for accuracy. The relative standard deviations （RSDs） of the inter-day＇s retention time and peak area of all species were less than 0.938% and 8.731%, respectively, while RSDs of 5-day retention time and peak area were less than 1.265% and 8.934%, respectively. The correlation coefficients for targeted analytes ranged from 0.999 7 to 1.000 0. The spiked recoveries for the anions were 90%-102.5%. We concluded that the method can be applied for comprehensive evaluation of commercial gargles.     

上古音系不必立祭废至队曷桓戈部

上古韵部划分是上古音研究的核心部分,韵部划分至清代虽已渐臻完备,但学界对韵部进行再划分的工作一直未断。李新魁先生在二十九部基础上分出祭、废、至、队、曷、桓、戈七部,将上古韵部分为三十六部,其分部的依据不合理,故不必立祭、废、至、队、曷、桓、戈七部。     

词义分析、理解、选择与确立的理论依据——兼谈翻译应力戒望文生义

分析了翻译中译者经常遇到的根本问题:词义的分析、理解、选择与确立。笔者从中探讨了解决这一问题的理论依据,并指出翻译应力戒望文生义。     

“皇、帝、君、王”同义辨析

＂皇、帝、君、王＂是一组同义词,但学者历来对此有不同理解。古汉语同义词同中有异,本文试图把本组同义词以先秦至汉作为共时层面,上溯下联,共时与历时相结合,在一个词义的系统中运用多手段、多角度、多侧面、综合型的方法,精确地辨析该组词义。     

美英法德俄五国课程表比较

学校教育是被时钟控制的,课程表就是这种控制的表现.我国现行课程中,课程表是学校惟一有权自主决定和安排的,然而限于传统的视野,即使在实行新课程改革的今日,许多学校往往意识不到课程表的价值.然而当我们把目光转向国外的学校时,却发现这个"螺蛳壳"中还是有许多"道场"可做的.本文从课程编制、教与学的组织及课程中的时间安排这三个角度,对五国的课程改革进行分析,希望能为我国的改革提供一些借鉴.