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1.
G. M. Rao P Sumita M Roshni M. N. Ashtagimatt 《Indian journal of clinical biochemistry : IJCB》2005,20(1):198-200
It has been suggested that antioxidant systems are impaired in pregnancy induced hypertension and hence patients are exposed
to oxidative stress. In order to investigate the relationship between lipid peroxidation and certain antioxidant parameters
in blood of pregnancy induced hypertension (PIH) cases, 25 normotensive and 23 PIH samples were studied. In the present study,
thiobarbituric acid reactive substances showed a tendency to increase, however the increase remained statistically insignificant.
Plasma ascorbic acid level remained unaltered and Vitamin E showed a tendency to increase in the study group. The findings
implicate oxidative stress in the disease and cite the biochemical rationale for clinical trials of antioxidants to prevent
and treat pregnancy induced hypertension. 相似文献
2.
S. B. Patil M. V. Kodliwadmath Sheela M. Kodliwadmath 《Indian journal of clinical biochemistry : IJCB》2007,22(1):135-137
Pregnancy is a physiological state accompanied by a high-energy demand and an increased oxygen requirement. Augmented levels
of oxidative stress would be expected because of the increased intake and utilization of oxygen. Evidence of increased oxidative
stress in normal pregnancy in comparison with nonpregnant controls was estimated by measuring Thiobarbituric acid reactive
substance (TBARS), enzymatic antioxidants like Superoxide dismutase, Glutathione peroxidase, Glutathione reductase and catalase.
It was observed that pregnant women were more susceptible to oxidative damage than non-pregnants as indicated by increased
TBARS and decreased antioxidants. 相似文献
3.
Akila V. Prashant H. Harishchandra Vivian D'souza Benedicta D'souza 《Indian journal of clinical biochemistry : IJCB》2007,22(1):131-134
Advanced age is associated with an accumulation of free radical damage, which leads to physiological and clinical modifications.
Age related changes resulting from free radical reactions include increasing levels of lipid peroxides, alterations in enzyme
activities and greater osmotic fragility. The present study was conducted to estimate the level of lipid peroxidation product-Malondialdehyde
and antioxidants Catalase and Glutathione in elderly people. An increase in lipid peroxidation and decrease in antioxidants
was observed in normal elderly people. Highly significant increase in MDA and decrease in antioxidants was observed in elderly
people when complicated with diabetes and hypertension. Supplementation of antioxidants may prevent further oxidative injury
in elderly people. 相似文献
4.
Hem Lata G. K. Ahuja A. P. S. Narang Lily Walia 《Indian journal of clinical biochemistry : IJCB》2004,19(2):1-4
Stress is one of the basic factors in the aetiology of a number of diseases such as atherosclerosis, coronary heart disease,
aging, liver disease etc. Hence this work was designed to study the effect of stress in the form of forced immobilisation
stress on lipid peroxidation and lipid profile in rabbits. The study was conducted in 25 healthy rabbits of either sex. Rabbits
were subjected to forced immobilisation for two hours everyday for consecutive seven days. Blood samples were collected on
day 1, 3 and 7 after immobilisation period. Malondialdehyde (MDA), a marker of lipid peroxidation was estimated by TBA assay
method. Lipid profile was analysed by multichannel autoanalyser. There was statistically significant increase in MDA, total
cholesterol, LDL, VLDL and triglycerides with immobilisation. These findings indicate that stress in the form of forced immobilisation
increases lipid peroxidation and alter lipid profile, which may be responsible for pathophysiology of various diseases. 相似文献
5.
J Kato Alice Abraham Ruram S Sekharjit Singh S Bilasini Devi Th Ibetombi Devi W Gyaneshwar Singh 《Indian journal of clinical biochemistry : IJCB》2007,22(1):128-130
The present, study was conducted to determine the level of malondialdehyde (MDA) as an index of free radial induced lipid
peroxidation and antioxidant vitamins-vitamins A, vitamin C and vitamin E in 75 confirmed cases of urolithiasis. Significantly
high level of MDA (p<0.001) with significantly low levels of vitamin E (p<0.001) and vitamin A (p<0.001) with no significant
decrease in vitamin C (p>0.05) were observed in the plasma of urolithiasis cases as compared to normal controls. In conclusion,
it appears that a role of lipid peroxidation and oxidative function exists in the pathogenesis of urolithiasis. But, the exact
mechanism how this occurs remains to be elucidated. 相似文献
6.
Shruti Mohanty Nalini Nayak N. N. Nanda Pragna Rao 《Indian journal of clinical biochemistry : IJCB》2006,21(1):189-192
Background Pregnancy induced hypertension (PIH) contributes to 15.6% maternal mortality in India. In Behrampur, Orissa, maternal deaths
due to PIH was 32%, which is twice the national incidence. Hence in this population, some factors associated with severity
of PIH were studied. Serum lipid concentrations and malondialdehyde (MDA) levels were correlated with severity of PIH and
birth weight of the neonate.
Patients & Methods 70 primiparous PIH patients and 20 healthy controls were studied. Serum lipids and MDA were estimated. Maternal blood pressures
and birth weights of the neonate were recorded.
Results and Conclusion Serum cholesterol, triglyceride, LDL, VLDL and MDA were significantly elevated in primiparous PIH patients when compared to
control subjects. Average birth weight of babies born to mothers with PIH was less than those born to control subjects. The
most significant factor was that in this geographical area, 26% of the primiparous patients with PIH were below 20 years of
age while only 15% of the controls were less than 20 years, indicating that younger maternal age was a contributing factor
to PIH. 相似文献
7.
Jayanta De Ananda Mukhopadhyay Pradip Kumar Saha 《Indian journal of clinical biochemistry : IJCB》2006,21(2):165-168
Four groups of subjects: normal healthy normotensive nonpregnant women (Group A), normal normotensive pregnant women (Group
B), women with preeclamptic toxaemia (Group C) and eclamptic women (Group D): with fifty subjects in each group, were investigated
for serum lipid profile in the third trimester of pregnancy. There was significant increase in serum triglyceride and VLDL
cholesterol leve as well as decrease in LDL cholesterol in normal pregnancy, while total cholesterol and HDL cholesterol levels
did not show any statistically significant alteration. The preeclampsia (Group C) was associated with a significant rise in
triglyceride and VLDL cholesterol and fall in HDL cholesterol concentration, while eclamptic women showed significant fall
in HDL cholesterol and rise in LDL cholesterol as compared to normal pregnant women. However, interestingly, elevation of
the ratios of total cholesterol: HDL cholesterol and triglyceride: HDL cholesterol as well as diminuition of the ratio of
HDL cholesterol: VLDL cholesterol showed statistical significance in pregnancy induced hypertension in both Groups C and D,
while eclamptic women showed significant elevation of LDL cholesterol: HDL cholesterol ratio in addition. 相似文献
8.
Vaishali N. Thorat Adinath N. Suryakar Pradeep Naik Bipin M. Tiwale 《Indian journal of clinical biochemistry : IJCB》2009,24(1):102-104
Liver transplantation is an accepted therapy for chronic liver disease patients. These patients generally have low levels
of fat soluble vitamins, which have important antioxidant roles. Therefore, this study was undertaken to investigate whether
such patients had evidence of antioxidant depletion and increased lipid peroxidation before transplant and whether the subsequent
ischemia and reperfusion encountered during liver transplantation have any effect on antioxidant levels and lipid peroxidation.
We assessed plasma total antioxidant capacity and serum lipid peroxide in 12 patients undergoing liver transplantation and
equal numbers of healthy subjects. We found that before reperfusion, antioxidant levels were significantly decreased along
with significantly elevated lipid peroxidation levels as compared with healthy controls (P<0.001). On reperfusion of the liver
graft, further declined values of total antioxidant accompanied with highly elevated lipid peroxidation were seen than those
of pre-reperfusion samples (P<0.001). This data shows that patients undergoing liver transplant have lowered antioxidant defenses
and evidence of free radical damage, which compound the additional insult of reperfusion injury. Therefore antioxidant therapy
in these patients before transplantation may ameliorate the effects of reperfusion. 相似文献
9.
Hilda Priya D'Souza H. Ramachandra Prabhu 《Indian journal of clinical biochemistry : IJCB》2006,21(2):138-141
The beneficial effects of ∈-3 fatty acids on human health have been well documented. Fish and fish oils are the richest sources
of ∈-3 fatty acids. However, due to their high degree of unsaturation, they are highly susceptible to lipid peroxidation.
Regular consumption of peroxidised oils may represent a risk factor for the induction and development of atherosclerosis.
In view of the above reports, it was considered necessary to study the effects of tumeric on fish lipid peroxidation during
standard cooking practices and on time-dependent changes in the peroxidation of fish homogenate. The antioxidant effect of
α-tocopherol was also studied to confirm the relevance of the study. The results suggest that turmeric may be considered as
a safe, cheap and readily usable antioxidant for food preparations. 相似文献
10.
A. P. Jain A. Mohan O. P. Gupta U. N. Jajoo S. P. Kalantri L. M. Srivastava 《Indian journal of clinical biochemistry : IJCB》1998,13(2):119-122
In view of sufficient body of evidence to substantiate the role of free radical mediated oxidative injury in myocardial infarction
(M.I.), the utility of exogenous supplementation with antioxidant substance, vitamin E was studied. Forty patients of acute
myocardial infarction were divided into two equal groups, one who received vitamin E and the other who did not. Plasma MDA
levels showed a reduction in patients of M.I. who received vitamin E as well as in those who did not, however, the decrease
was significantly more in the vitamin E treated subgroup. 相似文献
11.
Anjali C. Warrier Narasimha Y. Rao Devdutta S. Kulpati Tarun K. Mishra Bhaskar C. Kabi 《Indian journal of clinical biochemistry : IJCB》1995,10(1):9-13
There was increase in erythrocyte lipid peroxidation and adenosine deaminase (ADA) activity in red blood cells (RBC's), white
blood cells (WBC's) and plasma, in 30 patients of confirmed diabetes mellitus, 10 each of insulin dependent diabetes mellitus
(IDDM or type I), non-insulin dependent diabetes mellitus (NIDDM or type II) and diabetes with ketoacidosis when compared
with 20 healthy individuals (controls). Glycosylated hemoglobin A1c%, plasma free fatty acid (FFA) and glycerol levels were
also elevated in all the cases when compared with controls. The increase was more in diabetic ketoacidosis group. Significant
positive correlations were seen between erythrocyte lipid peroxidation and ADA activities in RBC's, WBC's and plasma. It is
suggested that decreased tissue adenosine levels due to increase in ADA activity, is related to the severity of hyperglycemia
and lipid peroxidation in diabetes mellitus. 相似文献
12.
N. P. Suryawanshi A. K. Bhutey A. N. Nagdeote A. A. Jadhav G. S. Manoorkar 《Indian journal of clinical biochemistry : IJCB》2006,21(1):126-130
The study was designed to find out the correlation between lipid peroxidation, lipoprotein levels to severity and complication
of diabetes mellitus. Degree of lipid peroxidation was measured in terms of malondialdehyde (MDA) along with lipid profile
and blood glucose in diabetes mellitus. It is categorised into insulin dependent diabetes mellitus (IDDM), non insulin dependent
diabetes mellitus (NIDDM) and diabetes mellitus(DM) with complication.
Total 112 known diabetic patients and 52 non-diabetic controls were studied. These patients were grouped as per the concentration
of fasting blood glucose level i.e. controlled, poorly controlled, and uncontrolled group. There are significant increase
in the lipid peroxide (MDA) and lipid profile except HDL cholesterol which is decreased, has been found in all groups as compared
to controls In NIDDM group lipid peroxidation was markedly increased than IDDM group and it was higher in DM with complications.
Other finding observed was that the level of lipid peroxide increased as per the increase in concentration of blood glucose.
The increase lipid peroxidation in the hyperglycemic condition may be explained, as the superoxide dismutase enzyme which
is antioxidant becomes inactive due the formation of superoxide radical within the cell. Maximum lipid peroxidation leads
to the damage of the tissue and organs which results into complication in diabetic patients. High levels of total cholesterol
appear due to increased cholesterol synthesis. The triglyceride levels changes according to the glycemic, control. The increase
may be due to overproduction of VLDL-TG.
It is concluded that good metabolic control of hyperglycemia will prevent in alteration in peroxidation and the lipid metabolism,
which may help in good prognosis and preventing manifestation of vascular and secondary complication in diabetes mellitus. 相似文献
13.
S. Mohanty P. K. Sahu M. K. Mandal P. C. Mohapatra A. Panda 《Indian journal of clinical biochemistry : IJCB》2006,21(1):101-105
This work was undertaken to investigate correlation between oxidative stress and initiation of pathogenesis of pregnancy induced
hypertension (PIH). Fifty primigravidae in age group of 20–35 years and gestational age 28–42 weeks with PIH were taken as
cases. Twenty healthy primigravidae with no medical and surgical complications of pregnancy and with blood pressure ≤140/90
mm Hg served as controls. The cases were again subgrouped as severe preeclampsia (12 in number) and mild pre-eclampsia (38
in number). All of them were evaluated for serum malondialdehyde (MDA), Serum vitamin E and plasma vitamin C levels. The serum
MDA levels were raised significantly in women with mild preeclampsia (P<0.01) and in women with severe preeclampsia (P<0.01)
in comparison to normal primi gravida. The serum vitamin E levels were decreased in primi gravida with mild preeclampsia (p<0.1)
and in primi with severe pre eclampsia (P<0.1) in comparison to normal primi gravida but the fall was not statistically significant.
There was a significant fall (P<0.05) in the vitamin C levels in primi with mild preeclampsia than in the normal primi. The
vitamin C levels in severe preeclamptic patients were lower than the normal primi but the fall was not statistically significant
(P=0.10). The serum MDA and vitamin E showed a negative correlation in all the cases. The serum MDA and plasma vitamin C also
showed a negative correlation in the control and study group. This observation suggests that in hypertensive disorders of
pregnancy there is an imbalance between lipid peroxidation and antioxidant vitamin status because of oxidative stress. The
decreased serum concentrations of the antioxidant vitamins supports the hypothesis that lipid peroxidation is an important
causative factor in the pathogenesis of preeclampsia. The rise in antioxidants is probably to compensate the increased peroxide
load in severe preeclampsia. 相似文献
14.
Prasannachandra Vivian D'Souza Benedicta D'Souza 《Indian journal of clinical biochemistry : IJCB》2006,21(2):103-106
Reactive oxygen species are thought to be involved in the pathogenesis of Malaria. To assess the extent of oxidative stress,
a study was conducted in patients withPlasmodium falciparum malaria andPlasmodium Vivax malaria. Plasma Thiobarbituric acid reactive substances (TBARS) were measured to assess the degree of lipid peroxidation.
Antioxidant status was measured by estimating the levels of Vitamins E and C. Results were compared with age and sex matched
control subjects. This study suggests that plasma TBARS levels were significantly increased in malaria patients. The patients
withP. falciparum infection showed significantly increased levels of lipid peroxides when compared toP. vivax malaria. The antioxidant Vitamins E and C were decreased significantly in malaria patients in both the groups. Maximum decline
in Vitamin C was observed inP. vivax malaria. Therefore it is been hypothesized that antioxidant Vitamins E and C could provide protection against the oxidative
stress induced by malaria. 相似文献
15.
Solomon Genet Yakobo Lema Janne Lutale 《Indian journal of clinical biochemistry : IJCB》2013,28(2):177-180
In diabetes, persistent hyperglycemia results in increased production of free radicals especially oxygen free radicals, which can cause cell destruction and tissue injury resulting in cell dysfunction. With the premise that oxidative stress is a major cause of diabetic complications, we conducted a controlled laboratory based investigation on level of lipid peroxide levels in the serum of Type 1 and Type 2 diabetic patients attending Muhimbili National Hospital. From our clinical data it was observed that majority of the patients had higher waist to hip ration and body mass index, which suggests that the patients were either overweight or obese. The enrolled diabetic patients had higher lipid peroxide levels than controls and also Type 2 patients had higher lipid peroxide levels than Type 1 patients. Moreover, patients with known complications had higher lipid peroxide levels than patients without complications. The lipid peroxide levels in the diabetic patients were significantly different from that of the control subjects enrolled in the study. A majority of the diabetic patients had a poorly controlled blood sugar. Our finding hints that despite the fact that diabetic patients in our clinic are on follow up, they are at a risk of developing coronary heart diseases, neuropathy and other secondary diabetic complications. 相似文献
16.
K. S. Motghare Anil Bhutey B. B. Murrhar Madhur Gupta A. W. Meshram Y. Balsubramanium 《Indian journal of clinical biochemistry : IJCB》2001,16(2):213-215
With the growing interest in the concept of free radicals in the pathogenesis of myocardial ischemia, it was thought worthwhile
to study the changes in lipid peroxides and antioxidant enzyme glutathione peroxidase (GPx) in ischemic heart disease (IHD).
The study was carried out on 76 patients of IHD-38 of acute myocardial infarction (AMI) and 38 of stable ischemic heart disease
(SIDH). They were age and sex matched with 38 normal healthy controls. A significant increase (p<0.001) in lipid peroxides
as malondialdehyde (MDA) (5.9±0.7 mmol/L) and a decrease in GPx (24.6±2.2 U/gmHb) was found in patients of AMI when compared
with controls. There was no significant difference in these values in SIHD. Thus this study confirms the earlier findings
that MDA and GPx are useful parameters in IHD and their magnitude is dependent on severity and/or duration of ischemia. We
suggest that these tests would be of use in smaller institutions with limited facilities. 相似文献
17.
Manzoor Ahmad Nida Suhail Tariq Mansoor Naheed Banu Shamshad Ahmad 《Indian journal of clinical biochemistry : IJCB》2012,27(4):385-388
In the present study, oxidative stress and lymphocytic DNA damage in both pre-op and post-op benign prostrate hyperplasia (BPH) patients with age >50 years was evaluated and compared with normal healthy subjects (controls- without any evidence of disease) of the same sex and age group. From December 2007 to November 2009, oxidative stress in 45 BPH patients were evaluated both before (pre-op patients) and after 7 days of surgery (post-op patients) in terms of measurements of plasma levels of (1) various anti-oxidative enzymes, (2) non-enzymatic antioxidants and (3) malondialdehyde which is a product of lipid peroxidation. The lymphocyte DNA damage was also evaluated by single cell alkaline gel electrophoresis in terms of tail length migration in these patients. These values were compared with their respective control subjects of similar sex and age group. The activities of antioxidant enzymes and the levels of antioxidant, reduced glutathione were found significantly decreased (p < 0.05) in serum samples of pre-operative group of BPH patients as compared to the controls. These altered parameters increased significantly (p < 0.05) and returned to their near normal control values, but not up to baseline values, in post operative patients i.e. after the cancer load was decreased by surgery. Lymphocytic DNA damage was found to be significantly increased in pre-op group as compared to controls and was reduced after surgery in post-op group. The present study therefore, shows significantly increased levels of oxidative stress and DNA damage in BPH patients which were reduced after removal of tumour load. Thus oxidative damage plays an important role in prostate tumourogenesis and timely management of oxidative stress can be of importance in preventing the occurrence of BPH. 相似文献
18.
A. K. Pradhan A. K. Shukla M. V. R. Reddy N. Garg 《Indian journal of clinical biochemistry : IJCB》2004,19(1):83-87
Oxidative stress was assesed by estimating lipid peroxidation product (LPO) in the form of thiobarbituric acid reactive substances
(TBARS), enzymatic antioxidants in the form of superoxide dismutase (SOD), catalase and nonenzymatic antioxidant vitamins
e.g. vitamin C, β carotene and vitamin E in either serum or plasma or erythrocytes in 190 cases of age related cataract in
the age group of 50–80 years. 190 cases were grouped into three morphological types namely, 73 cases of cortical, 77 cases
of posterior subcapsular and 40 cases of nuclear cataract and values of LPO and antioxidants were compared with 78 cases of
age matched healthy control groups. Plasma TBARS levels were cataract cases when compared with control groups. There were
no significant differences in the erythrocyte levels of catalase and plasma levels of Vit E between cataract cases and control
groups. No significant changes of parameters were seen among three different morphological types of age related cataract.
The present study shows that the oxidative stress may play an important role in the age related cataract. 相似文献
19.
Free radical generation and lipid peroxidation among the dry cell industry workers exposed to carbon
K. K. Reddy T. P. K. Reddy B. V. Somasekharaiah K. Soorya Kumari 《Indian journal of clinical biochemistry : IJCB》1998,13(1):27-32
A total of 75 healthy male dry cell industry workers exposed to carbon for 5, 8 and 10 years were compared with 48 controls
matched for age and economic status with respect to free radical generation, lipid peroxidation and antioxidant levels. Lymphocytes
were seperated from the whole blood and used for estimation of free radicals and antioxidants. Plasma lipid peroxidation products
were estimated in the form of thiobarbituric acid reactive substances. Significant increase in free radical generation and
lipid peroxidation products were observed in carbon exposed population than controls, and the increase was found to be significant
with increase in the period of exposure. The levels of antioxidants i.e., superoxide dismutase (SOD) and catalase were found
to decrease with the increase in exposure to carbon in industrial workers. These results suggest that exposure to carbon augments
free radical generation, lipid peroxidation and promotes decline in antioxidant enzymes. 相似文献
20.
Rashmi Raghuvanshi M. Chandra P. C. Misra M. K. Misra 《Indian journal of clinical biochemistry : IJCB》2005,20(1):26-29
Platelets play important role in precipitating ischaemic myocardial syndromes in many ways. One of the consequences of ischaemic
diseases is excessive generation of oxygen derived free radicals that have numerous pathophysiological consequences. Platelet
pro-oxidant enzyme, xanthine oxidase is one of the sources of generation of free radicals. In the present paper, we report
the effect of administration of vitamin E along with aspirin on the levels of platelet xanthine oxidase and extent of free
radical mediated damage in the patients reperfused after myocardial infarction.
Our findings show that administration of 400 mg. vitamin E for six days along with 80 mg. aspirin has an excellent anti-oxidant
effect as evidenced by reduced platelet xanthine oxidase activity and lowering of malondialdehdye (MDA) levels which is an
index of the extent of free radical mediated damage. 相似文献