Thermoregulatory responses during prolonged upper body exercise in cool and warm conditions

The thermoregulatory responses of upper-body trained athletes were examined at rest, during prolonged arm crank exercise and recovery in cool (21.5 ± 0.9°C, 43.9 ± 10.1% relative humidity; mean ± s) and warm (31.5 &± 0.6°C, 48.9 - 8.4% relative humidity) conditions. Aural temperature increased from rest by 0.7 ± 0.7°C (P ? 0.05) during exercise in cool conditions and by 1.6 ± 0.7°C during exercise in warm conditions (P ? 0.05). During exercise in cool conditions, calf skin temperature decreased (1.5 ± 1.3°C), whereas an increase was observed during exercise in warm conditions (3.0 ± 1.7°C). Lower-body skin temperatures tended to increase by greater amounts than upper-body skin temperatures during exercise in warm conditions. No differences were observed in blood lactate, heart rate or respiratory exchange ratio responses between conditions. Perceived exertion at 45 min of exercise was greater than that reported at 5 min of exercise during the cool trial (P ? 0.05), whereas during exercise in the warm trial the rating of perceived exertion increased from initial values by 30 min (P ? 0.05). Heat storage, body mass losses and fluid consumption were greater during exercise in warm conditions (7.06 ± 2.25 J·g^-1 ·°C^-1, 1.3 ± 0.5 kg and 1038 ± 356 ml, respectively) than in cool conditions (1.35 ± 0.23 J·g^-1·°C^-1, 0.8 ± 0.2 kg and 530 ± 284 ml, respectively; P ? 0.05). The results of this study indicate that the increasing thermal strain with constant thermal stress in warm conditions is due to heat storage within the lower body. These results may aid in understanding thermoregulatory control mechanisms of populations with a thermoregulatory dysfunction, such as those with spinal cord injuries.     

Decreased energy expenditure during prolonged sub-maximal exercise in a warm environment

Abstract

The purpose of this study was to investigate the influence of a warm environment on thermoregulation and energy expenditure during sub-maximal prolonged exercise in humans. Six healthy male subjects cycled for 120?min at an intensity of 60% maximal oxygen uptake (Vo₂max) at three environmental temperatures (10°C, CT; 20°C, MT; and 30°C, WT). Although oxygen uptake at WT showed a significantly lower value compared to those at MT and CT, no significant differences of respiratory exchange ratio were observed among the three environmental trials. A remarkable decrease in total energy expenditure during the 120-min exercise at WT was observed in comparison with those at MT and CT (p<0.05). Changes in rectal temperature, mean skin temperature, and mean body temperature at WT were significantly higher than those at both MT and CT. Although increases in mean body temperature from rest every five minutes during exercise were not different among three environmental temperatures, mean energy expenditures every five minutes at WT were lower compared with those at MT and CT (p<0.05). These results suggest that the increase in energy expenditure for physical exertion is substantially reduced during prolonged sub-maximal exercise in a warm environment. This acute alteration in the energy metabolism may contribute to inhibition of excess heat production and enable prolonged exercise in a warm environment.     

Thermoregulatory responses to exercise: relative versus absolute intensity

The purpose of the present study was to re-examine the relationship between deep body temperature and relative exercise intensity, during running rather than cycling (Saltin and Hermansen, 1966). Twenty male competitive and recreational distance runners, aged 22 + 0.9 years (mean +/- sx), were selected to form two groups, one with high maximal oxygen uptake (VO2max) values (72.8 +/- 0.8 ml x kg(-1) x min(-1)) and the other with moderate values (59.4 +/- 0.7 ml x kg(-1) x min(-1)). The participants completed two 60 min constant-paced treadmill runs at a common speed (absolute intensity) of 10.5 km x h(-1) and at a relative exercise intensity at a speed equivalent to 65% of VO2max. During the relative exercise intensity trial, no differences were found in rectal temperature, skin temperature or heart rate between groups. However, when running at the common speed, differences were identified in rectal temperature. At 60 min, rectal temperature was 37.70 +/- 0.19 degrees C and 38.19 +/- 0.11 degrees C for the high and moderate VO2max groups, respectively (P < 0.05). Sweat lost was significantly higher in the moderate VO2max group (moderate: 1.05 +/- 0.06 kg x h(-1); high: 0.82 +/- 0.08 kg x h(-1); P < 0.05). Heart rates were also different between groups over the first 20 min during the common speed trial (P < 0.05). The results of the present study support the findings of Saltin and Hermansen (1966), in that the set-point at which temperature is maintained is related to the relative exercise intensity.     

The influence of drink temperature on thermoregulatory responses during prolonged exercise in a moderate environment

Nine males cycled at 53% (s = 2) of their peak oxygen uptake (VO(2peak)) for 90 min (dry bulb temperature: 25.4 degrees C, s = 0.2; relative humidity: 61%, s = 3). One litre of flavoured water at 10 (cold), 37 (warm) or 50 degrees C (hot) was ingested 30 - 40 min into exercise. Immediately after the 90 min of exercise, participants cycled at 95%VO(2peak) to exhaustion to assess exercise capacity. Rectal and mean skin temperatures and heart rate were recorded. The gradient of rise in rectal temperature was influenced (P < 0.01) by drink temperature. Mean skin temperature was highest in the hot trial (cold trial: 34.2 degrees C, s = 0.5; warm trial: 34.4 degrees C, s = 0.5; hot trial: 34.7 degrees C, s = 0.6; P < 0.01). Significant differences were observed in heart rate (cold trial: 132 beats . min(-1), s = 13; warm trial: 134 beats . min(-1), s = 12; hot trial: 139 beats . min(-1), s = 13; P < 0.05). Exercise capacity was similar between trials (cold trial: 234 s, s = 69; warm trial: 214 s, s = 52; hot trial: 203 s, s = 53; P = 0.562). The heat load and debt induced via drinking resulted in appropriate thermoregulatory reflexes during exercise leading to an observed heat content difference of only 33 kJ instead of the predicted 167 kJ between the cold and hot trials. These results suggest that there may be a role for drink temperature in influencing thermoregulation during exercise.     

Physiological and metabolic responses of wheelchair athletes in different racing classes to prolonged exercise

The aims of this study were to examine and compare selected physiological and metabolic responses of wheelchair athletes in two paraplegic racing classes [T3: n = 8 (lesion levels T1-T7; paraplegics); T4: n = 9 (lesion levels below T7; paraplegics)] to prolonged exercise. In addition, we describe the responses of three tetraplegic athletes [T2: n = 3 (lesion levels C6/C7: tetraplegics)]. Twenty athletes completed 90 min of exercise at 75% VO2peak on a motorized treadmill adapted for wheelchairs. The mean (+/- s) heart rates of the T3 and T4 racing classes were 165 +/- 2 and 172 +/- 6 beats.min-1, respectively. For the T4 racing class, heart rate gradually increased during the test (P < 0.05), whereas for the T3 racing class, heart rate reached a plateau after an initial increase. The mean heart rate of the tetraplegics was 114 +/- 3 beats.min-1. The T3 and T4 classes exhibited similar respiratory exchange ratios, plasma lactate and glucose concentrations throughout the test. For both the T3 and T4 racing class, free fatty acid, glycerol, ammonia, urea and potassium concentrations had increased from resting values by the end of the test (P < 0.05). In conclusion, the results of this study suggest that endurance-trained wheelchair athletes are able to maintain velocities equivalent to the same relative exercise intensity (75% VO2peak) for prolonged periods irrespective of lesion level.     

Whole-body pre-cooling and heat storage during self-paced cycling performance in warm humid conditions

The aim of this study was to establish the effect that pre-cooling the skin without a concomitant reduction in core temperature has on subsequent self-paced cycling performance under warm humid (31 degrees C and 60% relative humidity) conditions. Seven moderately trained males performed a 30 min self-paced cycling trial on two separate occasions. The conditions were counterbalanced as control or whole-body pre-cooling by water immersion so that resting skin temperature was reduced by approximately 5-6 degrees C. After pre-cooling, mean skin temperature was lower throughout exercise and rectal temperature was lower (P < 0.05) between 15 and 25 min of exercise. Consequently, heat storage increased (P < 0.003) from 84.0+/-8.8 W x m(-2) to 153+/-13.1 W x m(-2) (mean +/- s(mean)) after pre-cooling, while total body sweat fell from 1.7+/-0.1 l x h(-1) to 1.2+/-0.1 l h(-1) (P < 0.05). The distance cycled increased from 14.9+/-0.8 to 15.8+/-0.7 km (P < 0.05) after pre-cooling. The results indicate that skin pre-cooling in the absence of a reduced rectal temperature is effective in reducing thermal strain and increasing the distance cycled in 30 min under warm humid conditions.     

The influence of pre-warming on the physiological responses to prolonged intermittent exercise

To examine the influence of pre-warming on the physiological responses to prolonged intermittent exercise in ambient temperatures of 21.5 +/- 0.6 degrees C and relative humidities of 35.7 +/- 5.4% (mean +/- s), six healthy men performed intermittent treadmill running (30-s bouts at 90% of maximal oxygen uptake separated by 30-s static recovery periods) to exhaustion after active pre-warming, passive pre-warming and pre-exercise rest (control). Exercise time to exhaustion was significantly different between all conditions (active, 51.8 +/- 7.2 min; passive, 38.5 +/- 11.1 min; control, 72.0 +/- 17.2 min; P < 0.05). These changes in performance time were closely associated with a significant decline in both the rate of heat storage and heat storage capacity (P < 0.05). Rectal temperature, heart rate and ratings of perceived exertion were significantly higher during exercise in the two pre-warming conditions than in the control condition (P < 0.05). Ratings of perceived exertion were also significantly higher during exercise following passive pre-warming compared with active pre-warming (P < 0.05). During exercise there were no significant differences in serum prolactin, plasma norepinephrine and plasma free fatty acid concentrations between conditions. We conclude that both active and passive pre-warming promote a reduction in prolonged intermittent exercise capacity in environmental temperatures of 21 degrees C compared with pre-exercise rest. These performance decrements were dependent upon the mode of pre-warming and closely reflected alterations in body heat content.     

Cardiovascular and ventilatory responses during formalized T'ai Chi Chuan exercise

T'ai Chi Chuan (TCC) is a widely practiced Chinese martial art said to physically develop balance and coordination as well as enhance emotional and mental health. TCC consists of a series of postures combined into a sequential movement providing a smooth, continuous, low-intensity activity. The purpose of this study was to examine the ventilatory and cardiovascular responses to the Long Form of Yang's style TCC. In addition, the subjects' TCC responses were compared to their ventilatory and cardiovascular responses during cycle ergometry at an oxygen consumption (VO2) equivalent to the mean TCC VO2. Six experienced (M = 8.3 yrs) male TCC practitioners served as subjects with data collected during the Cloud H and movement of the TCC exercise. Significantly (p less than .05) lower responses for ventilatory frequency (Vf) (11.3 and 15.7 breaths.min-1), ventilatory equivalent (VE/VO2) (23.47 and 27.41), and the ratio of dead space ventilation to tidal volume (VD/VT) (20 and 27%) were found in TCC in comparison to cycle ergometry. The percentage of minute ventilation used for alveolar ventilation was significantly higher during TCC (p less than .03) than cycle ergometry, with mean values of 81.1% and 73.1%, respectively. Cardiac output, stroke volume, and heart rate were not significantly different between TCC exercise and cycle ergometry at the same oxygen consumption. We concluded that, during TCC, expert practitioners show significantly different ventilatory responses leading to more efficient use of the ventilatory volume than would be expected from comparable levels of exertion on a cycle ergometer.     

VO2 prediction and cardiorespiratory responses during underwater treadmill exercise

We compared cardiorespiratory responses to exercise on an underwater treadmill (UTM) and land treadmill (LTM) and derived an equation to estimate oxygen consumption (VO2) during UTM exercise. Fifty-five men and women completed one LTM and five UTM exercise sessions on separate days. The UTM sessions consisted of chest-deep immersion, with 0, 25, 50, 75, and 100% water-jet resistance. All session treadmill velocities increased every 3 min from 53.6 to 187.8 m x min(-1). Cardiorespiratory responses were similar between LTM and UTM when jet resistance for UTM was 50%. Using multiple regression analysis, weight-relative VO2 could be estimated as: VO2 (mLO2 c kg(-1) x min(-1)) = 0.19248 x height (cm) + 0.17422 x jet resistance (% max) + 0.14092 x velocity (m x min(-1)) -0.12794 x weight (kg)-27.82849, R2 = .82. Our data indicate that similar LTM and UTM cardiorespiratory responses are achievable, and we provide a reasonable estimate of UTM VO2.     

耐力项目练习者在超大强度运动中儿茶酚胺类激素的反应

为了比较男女耐力项目练习者在一次超最大强度运动时的交感肾上腺髓质活动水平。对14名(男女各7名)从事多年长跑训练的练习者，在运动前、运动后即刻和运动停止后5min分别采血测定肾上腺素(E)和去甲肾上腺素(NE)水平。男生组在Wingate试验中显示较高的运动水平，而有关E和NE，男女两组没有显著差异。这些结果表明男女长跑练习者在Wingate试验中的交感神经活动水平和肾上腺髓质的分泌能力是相似的。     

Stress responses during aerobic exercise in relation to motivational dominance and state

We examined the hypothesis that congruence between motivational dominance and state results in optimal psychological responses and performance during exercise. Twenty participants (10 telic dominant and 10 paratelic dominant) rated their stress at 5 min intervals as they cycled on an ergometer at gas exchange threshold for 30 min in both telic and paratelic state manipulated conditions. Participants then performed a test to exhaustion at a resistance equivalent to 110% of VO(2max). The hypothesized interaction between condition and dominance was significant for internal tension stress, as paratelic dominants were more stressed than telic dominants when exercising in the telic state and telic dominants were more stressed than paratelic dominants when exercising in the paratelic state. Similarly, the condition × dominance interaction for internal stress discrepancy was significant, as paratelic dominants reported greater internal stress discrepancy exercising in the telic compared with the paratelic state. Findings are discussed in relation to the application of reversal theory for understanding stress responses during aerobic exercise.     

Maximal strength and cortisol responses to psyching-up during the squat exercise

We studied the effect of psyching-up on one-repetition maximum (1-RM) performance and salivary cortisol responses during the squat exercise. Ten men (age 21.6+/-1.4 years; mean+/-s) and ten women (age 22.4+/-2.8 years) with weight training experience of 4.5+/-2.0 years participated in this study. One-repetition maximum squats were performed on a Smith machine during each of two different intervention conditions that were counterbalanced and consisted of a free choice psych-up and a cognitive distraction. Saliva samples were obtained at the beginning of each test session and immediately after the final 1-RM attempt. No significant difference in 1-RM was identified between psyching-up (104+/-50 kg) and cognitive distraction (106+/-52 kg). Performing a 1-RM in the squat exercise significantly increased salivary cortisol concentrations during both conditions (P<0.05). There was no significant difference in salivary cortisol responses between conditions. These results suggest that psyching-up does not increase 1-RM performance during the squat exercise in strength-trained individuals.     

The effects of substrate and fluid provision on thermoregulatory and metabolic responses to prolonged exercise in a hot environment

A high ambient temperature reduces the capacity to perform prolonged exercise. Total carbohydrate oxidation is less, and thus glycogen depletion is not limiting. Fluid ingestion in the heat should, therefore, focus on maintenance of hydration status rather than on substrate provision. Six healthy males cycled to exhaustion at 60% of maximum oxygen consumption (VO2max) with no drink, ingestion of a 15% carbohydrate-electrolyte drink (1.45+/-0.29 litres) or ingestion of a 2% carbohydrate-electrolyte drink (3.12+/-0.47 litres). The ambient temperature was 30.2+/-0.6 degrees C (mean +/- s), with a relative humidity of 71+/-1% and an air speed of approximately 0.7 m x s(-1) on all trials. Weighted mean skin temperature, rectal temperature and heart rate were recorded and venous samples drawn for determination of plasma volume changes, blood metabolites, serum electrolytes and osmolality. Expired gas was collected to estimate rates of fuel oxidation. Exercise capacity was significantly (P < 0.05) different in all trials. The median (range) time to exhaustion was 70.9 min (39.4-97.4 min) in the no-drink trial, 84.0 min (62.7-145 min) in the 15% carbohydrate trial and 118 min (82.6-168 min) in the 2% carbohydrate trial. The 15% carbohydrate drink resulted in significantly (P < 0.05) elevated blood glucose and total carbohydrate oxidation compared with the no-drink trial. The 2% carbohydrate drink restored plasma volume to pre-exercise values by the end of exercise. No differences were observed in other thermoregulatory or cardiorespiratory responses between trials. These results suggest that fluid replacement with a large volume of a dilute carbohydrate drink is beneficial during exercise in the heat, but the precise mechanisms for the improved exercise capacity are unclear.     

The effects of substrate and fluid provision on thermoregulatory and metabolic responses to prolonged exercise in a hot environment

A high ambient temperature reduces the capacity to perform prolonged exercise. Total carbohydrate oxidation is less, and thus glycogen depletion is not limiting. Fluid ingestion in the heat should, therefore, focus on maintenance of hydration status rather than on substrate provision. Six healthy males cycled to exhaustion at 60% of maximum oxygen consumption (VO 2max ) with no drink, ingestion of a 15% carbohydrate-electrolyte drink (1.45 - 0.29 litres) or ingestion of a 2% carbohydrate-electrolyte drink (3.12 - 0.47 litres). The ambient temperature was 30.2 - 0.6°C (mean - s ), with a relative humidity of 71 - 1% and an air speed of approximately 0.7 m.s -1 on all trials. Weighted mean skin temperature, rectal temperature and heart rate were recorded and venous samples drawn for determination of plasma volume changes, blood metabolites, serum electrolytes and osmolality. Expired gas was collected to estimate rates of fuel oxidation. Exercise capacity was significantly ( P ? 0.05) different in all trials. The median (range) time to exhaustion was 70.9 min (39.4-97.4 min) in the no-drink trial, 84.0 min (62.7-145 min) in the 15% carbohydrate trial and 118 min (82.6-168 min) in the 2% carbohydrate trial. The 15% carbohydrate drink resulted in significantly ( P ? 0.05) elevated blood glucose and total carbohydrate oxidation compared with the no-drink trial. The 2% carbohydrate drink restored plasma volume to pre-exercise values by the end of exercise. No differences were observed in other thermoregulatory or cardiorespiratory responses between trials. These results suggest that fluid replacement with a large volume of a dilute carbohydrate drink is beneficial during exercise in the heat, but the precise mechanisms for the improved exercise capacity are unclear.     

Exhaled nitric oxide in single and repetitive prolonged exercise

This study was performed to determine the influence of single and repetitive exercise on nitric oxide (NO) concentration in the lung. Exhaled NO concentration (FE(NO)) was measured during a constant-flow exhalation manoeuvre (170 ml x s(-1), against a 10 cmH2O resistance) in healthy individuals (a) during and after a 100-min square-wave exercise of between 25 and 60% of maximal power output (n = 18) and (b) before and after five successive prolonged exercises (90-120 min, 75-85% of maximal heart rate) separated by 48 or 24 h (n = 8). The FE(NO0.170) was decreased during and after the 100-min exercise test (mean +/- s(x): 58.5 +/- 3.7% and 76.7 +/- 5.2% of resting value at 90 min of exercise and 15 min post-exercise, respectively; P < 0.05). The five successive exercise sessions induced a similar post-exercise FE(NO0.170) decrement (73.1 +/- 2.9% of resting value 15 min post-exercise), while basal FE(NO0.170) values were not different between the five sessions (P > 0.05). These results suggest that prolonged exercise induces a reduction in NO concentration within the lung that lasts for several minutes after the end of exercise. However, repetitive exercises (at least every 24 h) allow complete NO recovery from one session to another. The implication of such a decrease in NO availability within the lung remains to be clarified.     

Graduated compression stockings: physiological and perceptual responses during and after exercise

The aim of this study was to examine the effect of wearing graduated compression stockings on physiological and perceptual variables during and after intermittent (Experiment 1) and continuous (Experiment 2) running exercise. Fourteen recreational runners performed two multi-stage intermittent shuttle running tests with 1 h recovery between tests (Experiment 1). A further 14 participants performed a fast-paced continuous 10-km road run (Experiment 2). Participants wore commercially available knee-length graduated compression stockings (pressure at ankle 18 - 22 mmHg) beneath ankle-length sports socks (experimental trials) or just the latter (control trials) in a randomized counterbalanced design (for both experiments). No performance or physiological differences were observed between conditions during intermittent shuttle running. During the 10-km trials, there was a reduction in delayed-onset muscle soreness 24 h after exercise when wearing graduated compression stockings (P < 0.05). There was a marked difference in the frequency and location of soreness: two participants in the stockings trial but 13 participants in the control trial indicated soreness in the lower legs. Wearing graduated compression stockings during a 10-km road run appears to reduce delayed-onset muscle soreness after exercise in recreationally active men.     

Stability of pedalling mechanics during a prolonged cycling exercise performed at different cadences

The aim of this study was to analyse the effect of pedalling rate on the pattern of mechanical torque application and on neuromuscular fatigue during prolonged cycling exercise. Eleven well-trained individuals performed three 1-h pedalling sessions, at 50 rev.min-1, 110 rev.min-1 and a freely chosen cadence, at an intensity corresponding to 65% of their maximal aerobic power. The mechanical torque applied on the right pedal was recorded for 30 s every 5 min while pedalling. Contractile and neural properties of the quadriceps and hamstring muscles were analysed before and immediately after each of the three pedalling sessions. The post-exercise reduction in knee extensors maximal voluntary contraction was significant (P<0.01) irrespective of the cadence, but no difference was found between cadences. The use of a particular cadence did not lead to preferentially central or peripheral fatigue. An increase in cadence resulted in greater positive and negative work generated during pedalling. The mechanical pattern was not altered during the exercise, whatever the selected cadence. The present study demonstrates that despite the occurrence of neuromuscular fatigue, trained individuals maintained a stable pedalling pattern throughout an endurance cycling exercise for cadences ranging from 50 to 110 rev.min-1.     

Stability of pedalling mechanics during a prolonged cycling exercise performed at different cadences

The aim of this study was to analyse the effect of pedalling rate on the pattern of mechanical torque application and on neuromuscular fatigue during prolonged cycling exercise. Eleven well-trained individuals performed three 1-h pedalling sessions, at 50 rev?·?min^?1, 110 rev?·?min^?1 and a freely chosen cadence, at an intensity corresponding to 65% of their maximal aerobic power. The mechanical torque applied on the right pedal was recorded for 30?s every 5?min while pedalling. Contractile and neural properties of the quadriceps and hamstring muscles were analysed before and immediately after each of the three pedalling sessions. The post-exercise reduction in knee extensors maximal voluntary contraction was significant (P <?0.01) irrespective of the cadence, but no difference was found between cadences. The use of a particular cadence did not lead to preferentially central or peripheral fatigue. An increase in cadence resulted in greater positive and negative work generated during pedalling. The mechanical pattern was not altered during the exercise, whatever the selected cadence. The present study demonstrates that despite the occurrence of neuromuscular fatigue, trained individuals maintained a stable pedalling pattern throughout an endurance cycling exercise for cadences ranging from 50 to 110 rev?·?min^?1.     

Nutritional strategies for promoting fat utilization and delaying the onset of fatigue during prolonged exercise

Carbohydrate ingestion before and during endurance exercise delays the onset of fatigue (reduced power output). Therefore, endurance athletes are recommended to ingest diets high in carbohydrate (70% of total energy) during competition and training. However, increasing the availability of plasma free fatty acids has been shown to slow the rate of muscle and liver glycogen depletion by promoting the utilization of fat. Ingested fat, in the form of long-chain (C 16-22 ) triacylglycerols, is largely unavailable during acute exercise, but medium-chain (C 8-10 ) triacylglycerols are rapidly absorbed and oxidized. We have shown that the ingestion of medium-chain triacylglycerols in combination with carbohydrate spares muscle carbohydrate stores during 2 h of submaximal (< 70% VO 2 peak) cycling exercise, and improves 40 km time-trial performance. These data suggest that by combining carbohydrate and medium-chain triacylglycerols as a pre-exercise supplement and as a nutritional supplement during exercise, fat oxidation will be enhanced, and endogenous carbohydrate will be spared. We have also examined the chronic metabolic adaptations and effects on substrate utilization and endurance performance when athletes ingest a diet that is high in fat (> 70% by energy). Dietary fat adaptation for a period of at least 2-4 weeks has resulted in a nearly two-fold increase in resistance to fatigue during prolonged, low- to moderate-intensity cycling (< 70% VO 2 peak). Moreover, preliminary studies suggest that mean cycling 20 km time-trial performance following prolonged submaximal exercise is enhanced by 80 s after dietary fat adaptation and 3 days of carbohydrate loading. Thus the relative contribution of fuel substrate to prolonged endurance activity may be modified by training, pre-exercise feeding, habitual diet, or by artificially altering the hormonal milieu or the availability of circulating fuels. The time course and dose-response of these effects on maximizing the oxidative contribution of fat for exercise metabolism and in exercise performance have not been systematically studied during moderate- to high-intensity exercise in humans.